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Mechanism of Membrane Electroporation and Vesicle Deformation

  • Sergej Kakorin
  • Eberhard Neumann

Abstract

Membrane electroporation (ME) defines an electrical technique to render lipid membranes porous and permeable, transiently and reversibly, by external voltage pulses. The „electro-deformation“ of lipid vesicles is possibly a mechanism to provide larger pores leading to transmembrane transport or causing membrane rupture. Recently, electrochemical relaxation data have provided evidence that ME of a vesicle suspension is rapidly coupled with vesicle elongation. Thus the electroporative changes in the membrane structure are optically enhanced by the vesicle deformation and can be recognized in the very early stages by electrooptics. In the meantime there are numerous applications of ME to manipulate cells, organelles and tissues in medicine2, cell biology and biotechnology3, yet the exact molecular mechanism of ME is not well understood, even very controversial. We propose a general chemical-thermodynamical approach to the quantitative description of cell membrane electroporation.

Keywords

Vesicle Suspension Pore Edge Condenser Plate Lipid Bilayer Vesicle Pore Formation Process 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Kakorin S, Stoylov S, Neumann E: Electro-optics of membrane electroporation in diphenylhexatriene doped lipid bilayer vesicles. Biophys Chem, 58:109–116 (1996)CrossRefGoogle Scholar
  2. 2.
    Heller R, Jaroszeski MJ, Glass LF, Messina JL, Rapaport DP, Deconti RC, Fenske NA, Gilbert RA, Mir LM, Reintgen DS: Phase I/II Trial for the treatment of cutaneous and subcutaneous tumors using electrochemotherapy. Cancer, 77: 964–971 (1996)CrossRefGoogle Scholar
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    Neumann E, Kakorin S, Tsoneva I, Nikolova B, Tomov T: Calcium-mediated DNA adsorption to yeast cells and kinetics of cell transformation. Biophys J, 71:868–877 (1996)CrossRefGoogle Scholar
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    Neumann E: The Relaxation Hysteresis of Membrane Electroporation. In: Electroporation and Electrofusion in Cell Biology. E. Neumann, A. E. Sowers and C. Jordan editors, Plenum, New York, pp 61–82 (1989)Google Scholar
  5. 5.
    Neumann E and Kakorin S: Electrooptics of membrane electroporation and vesicle shape deformation. Curr Opin Colloid Interface Sci, 1: 790–799 (1996)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Sergej Kakorin
    • 1
  • Eberhard Neumann
    • 1
  1. 1.Faculty of ChemistryUniversity of BielefeldBielefeldGermany

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