Physiological Gradients of Voltage as Controls of Neural Morphogenesis

  • Richard B. Borgens

Abstract

It has long been suspected that endogenous steady ionic currents and natural voltage gradients may play a role in the early development of animals and plants9,10,13,15. For example, the cytoplasmic localizations in germinating fucus eggs may be controlled by a steady transcellular calcium current11,18, and a steady outwardly directed ionic current predicts the exact locus of limb development in both frog and salamander embryos4,5,19.Transcellular currents also play a critical role in cell and tissue reactions to injury and in their subsequent regeneration1,3,14. These experiments also provide insights into the use of imposed voltage gradients as a clinical treatment for nervous system trauma3. More recently, new studies provide direct evidence for a controlling role of endogenous currents and voltages in vertebrate morphogenesis, particularly when we restrict our attention to the early nervous system.

Keywords

Germinate Luminal 

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References

  1. 1.
    R.B. Borgens What is the role of naturally produced electric current in vertebrate regeneration and healing? International Review of Cytology 76:245–298 (1982).CrossRefGoogle Scholar
  2. 2.
    R.B. Borgens and R. Shi Uncoupling histogenesis from morphogenesis in the vertebrate embryo by collapse of the transneural tube potential. Developmental Dynamics 203:456–467 (1995).CrossRefGoogle Scholar
  3. 3.
    R.B. Borgens (1992) Applied voltages in spinal cord reconstruction: history, strategies, and behavioral models. Spinal Cord Dysfunction,Vol. 3: Functional Stimulation. L.S. Illis, Ed., (Oxford Medical Publications, Oxford), Chapter 5. pp. 110–144.Google Scholar
  4. 4.
    R.B. Borgens, M.F. Rouleau, and L.E. DeLanney A steady efflux of ionic current predicts hind limb development in the axolotl. J. Exp. Zool. 228:491–503 (1983).CrossRefGoogle Scholar
  5. 5.
    R.B. Borgens, L. Callahan, and M. Rouleau The anatomy of axolotl flank integument during limb bud development with special reference to a transcutaneous current predicting limb formation. J. Exp. Zool. 244:203–214 (1987).CrossRefGoogle Scholar
  6. 6.
    K.B. Hotary, and K.R. Robinson, Endogenous electrical currents and the resultant voltage gradients in the chick embryo. Dev. Biol. 140:149–160 (1990).CrossRefGoogle Scholar
  7. 7.
    K.B. Hotary and K.R. Robinson The neural tube of the Xenopus embryo maintains a potential difference across itself. Developmental Brain Research.59:65–73 (1991).CrossRefGoogle Scholar
  8. 8.
    K.B. Hotary and K.R. Robinson A computerized 2-dimensional vibrating probe for mapping extracellular current patterns J. Neurosci. Methods. 43:55–67 (1992b).CrossRefGoogle Scholar
  9. 9.
    L.F. Jaffe Calcium Explosions as Triggers of Development, Ann of N.Y. Acad. Sci. 339, 86–101 (1980).ADSCrossRefGoogle Scholar
  10. 10.
    L.F. Jaffe The role of ionic currents in establishing developmental pattern Philos. Trans. R. Soc. Lond. [Riot]. B295, 553–566 (1981).ADSCrossRefGoogle Scholar
  11. 11.
    L. F. Jaffe (1990) The roles of intermembrane calcium in polarizing and activating eggs. in Mechanisms of Fertilization: Plants to Humans, B. Dale, Ed., (Springer, Berlin, 1990) pp. 389–417.Google Scholar
  12. 12.
    L.F. Jaffe and R. Nuccitelli An ultrasensitive vibrating probe for measuring steady extracellular currents. J. Cell Biol. 63:614–628 (1974).CrossRefGoogle Scholar
  13. 13.
    Jaffe, L.F. and R. Nuccitelli, (1977) Electrical controls of development Ann. Rev. Biophys and Bioeng. 6:445–76.CrossRefGoogle Scholar
  14. 14.
    L.S. Jenkins, B.S. Duerstock, and R.B. Borgens Reduction of the current of injury leaving the amputation inhibits limb regeneration in the red spotted newt. Developmental Biology 178:251–262 (1996).CrossRefGoogle Scholar
  15. 15.
    E.I. Lund Bioelectric Fields and Growth, (University of Texas Press, 1947)Google Scholar
  16. 16.
    M.E.M. Metcalf and R. B. Borgens Weak applied voltages interfere with amphibian morphogenesis and pattern. J. Exp. Zool. 268:322–338 (1994).Google Scholar
  17. 17.
    M.E.M. Metcalf, R. Shi, and R. B. Borgens Endogenous ionic currents and voltages in amphibian embryos. J. Exp. Zool. 268:307–322 (1994).CrossRefGoogle Scholar
  18. 18.
    R. Nuccitelli Physiological electric fields can influence cell motility, growth, and polarity. Adv. Cell Biol. 2:213–233 (1988).CrossRefGoogle Scholar
  19. 19.
    K.R. Robinson Endogenous electrical current leaves the limb and prelimb region of the Xenopus embryo. Dev. Bio. 79: 203-211 (1983).CrossRefGoogle Scholar
  20. 20.
    R. Shi and R.B. Borgens Embryonic neuroepithelium sodium transport, the resulting physiological potential, and cranial development. Dev. Biol. 65:105–116 (1994).CrossRefGoogle Scholar
  21. 21.
    R. Shi and R.B. Borgens Three dimensional gradients of voltage during development of the nervous system as invisible coordinates for the establishment of embryonic pattern. Developmental Dynamics 202:101–114 (1995).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Richard B. Borgens
    • 1
  1. 1.1244 VCPR Center for Paralysis ResearchPurdue UniversityWest LafayetteUSA

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