Advertisement

Experimental Factors Affecting Dielectric Spectroscopy Measurements on Cell Suspensions of Chinese Hamster Fibroblasts

  • Gianni F. Mariutti
  • Alessandro Polichetti
  • Roberta Pozzi

Abstract

Dielectric spectroscopy in the radiofrequency spectral region can be a suitable technique to study some properties of biological media, including ion exchange across cell membrane. Traditionally we used such technique to investigate the effects of some physical treatments (hyperthermia, ionizing radiations) on cell suspensions of Chinese Hamster fibroblasts (V-79). We observed, however, that the sample manipulation to obtain a homogeneous dispersed phase before each measurement can significantly affect the experimental results. This is a serious problem, in particular, when following the time course of a treatment on the same sample by means of a series of measurements, because of the adding effect of each manipulation. We investigated, therefore, which are the critical parameters affecting the experimental results. We successfully worked out such problems by modifying the experimental procedure.

Keywords

Dielectric Spectroscopy Measuring Cell Physical Treatment Sample Conductivity Sample Manipulation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    A. Bonincontro and G.F. Mariutti, Influence of hyperthermia, pH and culturing conditions on the electrical parameters of Chinese hamster V79 cells, Phys. Med. Biol. 33:557 (1988).CrossRefGoogle Scholar
  2. 2.
    A. Bonincontro, C. Mari, and G.F. Mariutti, Irreversible transition of the dielectric β-dispersion observed during a 45’ hyperthermie treatment on V79 fibroblasts, in: Electricity and Magnetism in Biology and Medicine,M. Blank, ed., San Francisco Press, San Francisco (1992).Google Scholar

Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Gianni F. Mariutti
    • 1
  • Alessandro Polichetti
    • 1
  • Roberta Pozzi
    • 1
  1. 1.Laboratorio di FisicaIstituto Superiore di SanitàRomeItaly

Personalised recommendations