Experimental Factors Affecting Dielectric Spectroscopy Measurements on Cell Suspensions of Chinese Hamster Fibroblasts
Dielectric spectroscopy in the radiofrequency spectral region can be a suitable technique to study some properties of biological media, including ion exchange across cell membrane. Traditionally we used such technique to investigate the effects of some physical treatments (hyperthermia, ionizing radiations) on cell suspensions of Chinese Hamster fibroblasts (V-79). We observed, however, that the sample manipulation to obtain a homogeneous dispersed phase before each measurement can significantly affect the experimental results. This is a serious problem, in particular, when following the time course of a treatment on the same sample by means of a series of measurements, because of the adding effect of each manipulation. We investigated, therefore, which are the critical parameters affecting the experimental results. We successfully worked out such problems by modifying the experimental procedure.
KeywordsDielectric Spectroscopy Measuring Cell Physical Treatment Sample Conductivity Sample Manipulation
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