Genotoxicity Tests

Application to Occupational Exposure as Biomarkers
  • Ali E. Karakaya
  • Semra Sardas
  • Sema Burgaz
Chapter
Part of the NATO ASI Series book series (NSSA, volume 302)

Abstract

Using biomarkers for screening in workers who had known or suspected contact with genotoxic chemicals can be useful in quantifying exposure and assessing genotoxic risk. In this research the genotoxic risk of the following Turkish occupational groups (furniture workers, nurses, operating room personnel, hospital sterilising staff, engine repair workers, road-paving workers, car painting workers and hair colorists) were evaluated. Results of some markers of exposure and cytogenetic biomarkers (sister chromatid exchange, micronucleus assay and comet assay) has been assessed in the above mentioned occupational groups.

Keywords

Dust Glutathione Toluene Cisplatinum Adduct 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. S. Abe and M. Sasaki (1982) SCE as an index of mutagenesis and /or carcinogenesis. In:A. Sandberg (Ed.), Sister Chromatid Exchange.Alan R.Liss.Inc., New York, pp.461–464.Google Scholar
  2. N. Aygün, S. Sardas, Y. Ünal, M. Gamli, N. Berk and A.E. Karakaya (1997) Assessment of induced DNA damage by anaesthetic gases in operating room personnel by single-cell gel electrophoresis technique. 2nd National Congress of Toxicology, April 3-6,Antalya,Turkey.Abs.P-76.Google Scholar
  3. J.M. Baden and V.F. Simmon (1980) Mutagenic effects of inhalation anaesthetics. Mutation Res., 75,169.PubMedCrossRefGoogle Scholar
  4. A. Bayhan, S. Burgaz and A.E. Karakaya (1987) Urinary thioether excretion in nurses at an oncologic department. J.Clin.Pharm. Therap., 12, 303–306.CrossRefGoogle Scholar
  5. S. Burgaz, A. Bayhan and A.E. Karakaya (1987) Urinary thioethers in cigarette smokers. J. Fac. Pharm. Gazi, 4, 63–67.Google Scholar
  6. S. Burgaz, R. Rezanko, S. Kara and A.E. Karakaya (1992) Thioethers in urine of sterilization personnel exposed to ethylene oxide, J.Clin Pharm. Therap., 17, 169–172.CrossRefGoogle Scholar
  7. S. Burgaz, P.J.A. Borm and F.J. Jongeneelen (1992) Evaluation of urinary excretion of 1-hydroxypyrene and thioethers in workers exposed to bitumen fumes, Int.Arch. Occup. Environ. Health, 63, 397–401.PubMedCrossRefGoogle Scholar
  8. S. Burgaz, A. Iscan, Z.K. Büyükbingöl, A. Bozkurt and A.E. Karakaya (1995) Evaluation of micronuclei in exfoliated urothelial cells and urinary thioether excretion of smokers, Mutation Res., 335, 163–169.PubMedCrossRefGoogle Scholar
  9. S. Burgaz, O. Erdem, B. Karahalil, A.E. Karakaya (1998) Cytogenetic biomonitoring of workers exposed to bitumen fumes. Mutation Res., in press.Google Scholar
  10. C. Burnett (1980) Evaluation of toxicity and carcinogenecity of hair dyes. J. Toxicol. Environ. Health, 6, 247–257.PubMedCrossRefGoogle Scholar
  11. L.P. Carrillo, L.T. Arreola, L.T. Sanchez, F.E. Torres, C. Jimenez, M. Cabrian, S. Waliszewski and O. Saldate (1996) Is DDT use a public health problem in Mexico Environ. Health. Perspect., 104, 584–588.CrossRefGoogle Scholar
  12. E. DeRosa, N. Cellini, G. Sessa, C. Saletti, G. Rausa, G. Marcuzzo and G.B. Bartolucci (1993) Biological monitoring of workers exposed to styrene and acetone. Int. Arch. Occup. Environ. Health, 65, 107–110.CrossRefGoogle Scholar
  13. CT. DeRosa, Y.W. Stevens, J.W. Wilson, A.A. Ademeyero, S.D. Buchanan, W. Cibulas, P.J. Hughes, M.M. Mumtaz, R.E. Neft, H.R. Pohl and M.M. Johnson (1993) The agency for toxic substances and disease registry’s role in development and application of biomarkers in public health service. Toxicol. Industr. Health, 9, 979–994.Google Scholar
  14. R. Doll and R. Peto (1981) The causes of cancer. Oxford University Press, Oxford.Google Scholar
  15. M. Fenech and A.A. Morley (1985) Measurement of micronuclei in lymphocytes. Mutation Res., 147, 29–36.PubMedCrossRefGoogle Scholar
  16. T.R. Fennell (1990) Biological markers of exposure to chemical carcinogens. CIIT Activities,10(1), 1–7.Google Scholar
  17. M.L. Fischman, E. C. Cadman and S. Desmond (1990) Occupational cancer. In Occupational Medicine (Ed. J. LaDou), Prentice-Hall, Connecticut, pp. 182–208.Google Scholar
  18. J.A. Heddle (1973) A rapid in vivo test for chromosomal damage. Mutation Res., 18,187–190.PubMedCrossRefGoogle Scholar
  19. J.A. Heddle, M.C. Cimino, M. Hayashi, F. Romagna, M.D., Shelby, J.D. Tucker, Ph. Vanparys and J.T. Macgregor (1991) Micronuclei as an index of cytogenetic damage: Past, present, and future. Environ Mol. Mutagen. 18,277–291.PubMedCrossRefGoogle Scholar
  20. R.F. Henderson, W.E. Bechtold, J.A. Bond and J.D. Sun (1989) The use of biological markers in toxicology. CRC Critical Rev. Toxicol., 20, 65–82.Google Scholar
  21. H. Ilbars, A.E. Karakaya and S. Burgaz (1997) Evaluation of possible genotoxic exposure of professional hair colorists by micronucleus assay. 2nd National Congress of Toxicology, April 3-6, Antalya, Turkey. Abs.P-83.Google Scholar
  22. International Agency for Research on Cancer (1983) IARC Monographs, Polynuclear Aromatic Compounds. Part 1. Chemical, environmental and experimental data.Vol.32, Lyon.Google Scholar
  23. International Agency for Research on Cancer (1985) IARC Monographs, Polynuclear compounds,Bitumens,Coaltars and Derived Products, Shale oils and Soots. Vol.35. Lyon.Google Scholar
  24. International Agency for Research on Cancer (1987) I ARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Overall Evaluations of Carcinogenicity. IARC Publication Supplement 7, Lyon.Google Scholar
  25. International Labour Organisation (1988) Occupational CancenPrevention and Control. Occupational Safety and Health Series No. 39, Geneva.Google Scholar
  26. F.J. Jongeneelen, R.B.M. Anzion, P.Th. Henderson (1987) Determination of hydroxylated metabolites of polycyclic aromatic hydrocarbons in urine. J.Chromatog., 413, 227–232.CrossRefGoogle Scholar
  27. B. Karahalil, S. Burgaz, G. Fisek and A.E. Karakaya (1998) Biological monitoring of young workers exposed to polycyclic aromatic hydrocarbons in engine repair workshops. Mutation Res., 412, 261–269.PubMedCrossRefGoogle Scholar
  28. A.E. Karakaya, B. Karahalil, M. Yylmazer, N. Aygün, S. Sardas and S. Burgaz (1997) Evaluation of genotoxic potential of styrene in furniture workers using unsaturated polyester resins. Mutation Res., 392, 261–268.PubMedCrossRefGoogle Scholar
  29. La Dou (1996) The role of multinationale corporations in providing occupational health and safety in developing countries. Int. Arch. Occup. Environ. Health, 68, 363–366.CrossRefGoogle Scholar
  30. National Institute of Health(1992) Human health and the environment some research needs. NIH Publication No. 92-3344.Google Scholar
  31. O. Östling and K.J. Johanson (1984) Microelectrophoretic study of radiation-induced DNA damages in individual cells. Biochem. Biophys. Res. Commun., 123, 291–293.Google Scholar
  32. F. Perera (1993) Biomarkers and molecular epidemiology of occupational related cancer. J. Toxicol. Environ. Health, 40, 203–215.PubMedCrossRefGoogle Scholar
  33. F. Perera and R.M. Whyatt (1994) Biomarkers and molecular epidemiology in mutation/cancer research. Mutation Res., 313, 117–129.PubMedCrossRefGoogle Scholar
  34. P. Perry and H.J. Evans (1975) Cytological detection of mutagen-carcinogen exposure by SCE. Nature, 258, 121–125.PubMedCrossRefGoogle Scholar
  35. H.C. Pitot and Y.P. Dragan (1996) Chemical carcinogenesis. In Casarett and Doull’s Toxicology. Fifth Edition (Ed C.D. Klaassen), McGraw-Hill, New York, pp.201–267.Google Scholar
  36. S. Şardaş, S. Gök and A.E. Karakaya (1991) Increased frequency of sister chromatid exchanges in the peripheral lymphocytes of cigarette smokers. Toxic. In vitro, 5, 263–265.CrossRefGoogle Scholar
  37. S. Şardaş, S. Gök and A.E. Karakaya (1991) Sister chromatid exchanges in lymphocytes of nurses handling antineoplastic drugs. Toxicol. Lett., 55, 331–335.Google Scholar
  38. S. Şardaş, H. Cuhruk, A.E. Karakaya and Y. Atakurt (1992) Sister chromatid exchanges in operating room personnel, Mutation Res., 279, 117–120.PubMedCrossRefGoogle Scholar
  39. S. Şardaş, A.E. Karakaya and Y. Furtun (1994) Sister chromatid exchanges in workers employed in car painting workshops. Int. Arch. Occup. Environ. Health, 66, 33–35.Google Scholar
  40. S. Şardaş, N. Aygün and A.E. Karakaya (1997) Genotoxicity studies on professional hair colorists exposed to oxidation hair dyes. Mutation Res., 394, 153–161.PubMedCrossRefGoogle Scholar
  41. N.P. Singh, M.T. McCoy, R.R. Tice and E.L. Schneider (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res., 17, 184–191.CrossRefGoogle Scholar
  42. M. Sorsa, K. Hemminki and H. Vainio (1985) Occupational exposure to anticancer drugs-Potential and real hazards. Mutation Res., 154, 135–149.PubMedCrossRefGoogle Scholar
  43. H.F. Stich and M.P. Rosin (1984) Micronuclei in exfoliated human cells as a tool for studies in cancer risk and cancer intervention. Cancer Lett., 22,241–253.PubMedCrossRefGoogle Scholar
  44. H. Vainio (1995) Carcinogenesis and its prevention. In Occupational Toxicology (ed. N.H. Stacey), Taylor&Francis, London, pp. 149–162.Google Scholar
  45. E. Ward (1995) Overview of preventable industrial causes of occupational cancer. Environ. Health Perspect., 103, 197–203.PubMedGoogle Scholar
  46. J.B. Ward and R.E. Henderson (1996) Identification of needs in biomarker research. Environ. Health. Perspect., 104,895–900.PubMedGoogle Scholar
  47. World Health Organization (1985) Environmental Health Criteria for Ethylene Oxide. Vol: 55, Geneva.Google Scholar

Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Ali E. Karakaya
    • 1
  • Semra Sardas
    • 1
  • Sema Burgaz
    • 1
  1. 1.Faculty of Pharmacy Department of ToxicologyGazi UniversityHipodromAnkaraTurkey

Personalised recommendations