Abstract
Sinorhizobium fredii strain USDA257 nodulates the soybean cultivar Peking, but it fails to infect the cultivar McCall. Disruption of no1XWBTUV, a symbiosis plasmid locus, allows the bacterium to form nitrogen-fixing nodules on McCall. The flavonoid genistein induces expression of no1X and no1BTUV and the secretion of at least proteins from USDA257. We have discovered that this flavonoid signal also leads to the production of fibrillar, pilus-like appendages on the surface of the bacterial cell. We have purified these appendages and employed immunocytochemistry to show that three proteins, including No1X, are physically associated with them. These results were confirmed by Western blots with purified pilus proteins. The S. fredii proteins are immunologically distinct from HrpA, a structural protein from the Hrp pilus of Pseudomonas syringae pv. tomato.
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© 1999 Springer Science+Business Media New York
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Pueppke, S.G., DeBoer, M., He, XT., Krishnan, H.B. (1999). An Isoflavone from Soybean Roots Induces Sinorhizobium Fredii Strain USDA257 to Produce Pilus-Like Surface Appendages. In: Martĺnez, E., Hernández, G. (eds) Highlights of Nitrogen Fixation Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4795-2_25
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DOI: https://doi.org/10.1007/978-1-4615-4795-2_25
Publisher Name: Springer, Boston, MA
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