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Hepoxilin A3 is Metabolized Into its ω-Hydroxy Metabolite by Human Neutrophils

  • Cecil R. Pace-Asciak
  • Denis Reynaud
  • Olga Rounova
  • Peter Demin
  • Kazimir K. Pivnitsky
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 469)

Abstract

Hepoxilins (Hx) elicit a variety of biological actions based on their ability to affect ion movements in the cell [Pace-Asciak 1994; Pace-Asciak et al., 1995a; Pace-Asciak et al., 1995b]. The most notable of these actions includes the release of insulin from pancreatic islets [Pace-Asciak and Martin 1984] as well as the regulation of cell volume in platelets [Margalit et al., 1993]. In the human neutrophil, HxA3 releases calcium from intracellular stores and this release is Hx-receptor mediated [Reynaud et al., 1996; Reynaud et al., 1995]. During the course of our investigations in neutrophils, we noted that radiolabeled HxA3 was metabolized into a single product which was identified as the corresponding ω-hydroxy product retaining the basic hydroxy-epoxide functional group of the parent hepoxilin intact [Reynaud et al., 1997]. Additional studies reported herein also indicate that the hepoxilin ω-hydroxylase is different from that which metabolizes LTB4.

Keywords

Human Neutrophil Trimethylsilyl Ether Hydroxy Metabolite Pentafluorobenzyl Ester Epoxide Functional Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Cecil R. Pace-Asciak
    • 1
    • 2
  • Denis Reynaud
    • 1
  • Olga Rounova
    • 1
  • Peter Demin
    • 1
    • 3
  • Kazimir K. Pivnitsky
    • 3
  1. 1.Research InstituteHospital for Sick ChildrenTorontoCanada
  2. 2.Department of Pharmacology, Faculty of MedicineUniversity of TorontoCanada
  3. 3.N.D. Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussian Federation

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