Hepoxilin A3 is Metabolized Into its ω-Hydroxy Metabolite by Human Neutrophils
Hepoxilins (Hx) elicit a variety of biological actions based on their ability to affect ion movements in the cell [Pace-Asciak 1994; Pace-Asciak et al., 1995a; Pace-Asciak et al., 1995b]. The most notable of these actions includes the release of insulin from pancreatic islets [Pace-Asciak and Martin 1984] as well as the regulation of cell volume in platelets [Margalit et al., 1993]. In the human neutrophil, HxA3 releases calcium from intracellular stores and this release is Hx-receptor mediated [Reynaud et al., 1996; Reynaud et al., 1995]. During the course of our investigations in neutrophils, we noted that radiolabeled HxA3 was metabolized into a single product which was identified as the corresponding ω-hydroxy product retaining the basic hydroxy-epoxide functional group of the parent hepoxilin intact [Reynaud et al., 1997]. Additional studies reported herein also indicate that the hepoxilin ω-hydroxylase is different from that which metabolizes LTB4.
KeywordsHuman Neutrophil Trimethylsilyl Ether Hydroxy Metabolite Pentafluorobenzyl Ester Epoxide Functional Group
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