Abstract
Leukotriene B4 (LTB4) is a potent proinflammatory and chemotactic factor biosynthesized in various tissues, which is involved in inflammation, immune responses and host defense against infection.1 LTB4 activates inflammatory cells by binding to its cell-surface receptor (BLT). Specific binding for [3H]LTB4 was reported in the membrane fractions of granulocytes, T lymphocytes, alveolar macrophages, spleen, brain, THP-1 cells, and differentiated HL-60 cells.2–5 By addition of GTPγS, low affinity sites of [3H]LTB4 binding appeared in spleen membranes.5 LTB4 stimulated a dose-dependent increase in GTP hydrolysis in HL-60 cells.6 LTB4 induced increase in intracellular calcium, D-myo-inositol-l,4,5-triphosphate (InsP3) accumulation, O2-production, and activation of GTPase in a pertussis toxin (PTX)-sensitive manner.7,8 On the other hand, LTB4-induced hyperadhesiveness of vascular endothelial cells was reported to be PTX-insensitive.9 These data indicated the presence of BLT coupled with G-protein(s). However, BLT had not been purified to homogeneity or cDNA-cloned.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
Refferences
B. Samuelsson, S.E. Dahlen, J.A. Lindgren, C.A. Rouzer and C.N. Serhan, Leukotrienes and lipoxins: structures, biosynthesis, and biological effects, Science,237: 1171, (1987)
J.P. Cristol, B. Provencal and P. Sirois, Leukotriene receptors, J Recept Res,9: 341, (1989)
A.J. de Brum-Fernandes, G. Guillemette, P. Borgeat and P. Sirois, Specific binding sites for leukotriene B4 in guinea pig brain membranes, J Pharmacol Exp Ther,259: 1035, (1991)
Y. Harada, Effect of leukotriene B4 on enhancement of Superoxide production evoked by formyl-methionyl-leucyl-phenylalanine in myeloid differentiated HL-60 cells: possible involvement of intracellular calcium influx and high affinity receptor for leukotriene B4, Hiroshima J Med Sci,39: 89, (1990)
I. Miki, T. Watanabe, M. Nakamura, Y. Seyama, M. Ui, F. Sato and T. Shimizu, Solubilization and characterization of leukotriene B4 receptor-GTP binding protein complex from porcine spleen, Biochem Biophys Res Commun,166: 342, (1990)
K.R. McLeish, P. Gierschik, T. Schepers, D. Sidiropoulos and K.H. Jakobs, Evidence that activation of a common G-protein by receptors for leukotriene B4 and N-formylmethionyl-leucyl-phenylalanine in HL-60 cells occurs by different mechanisms, Biochem J,260: 427, (1989)
T. Andersson, W. Schlegel, A. Monod, K.H. Krause, O. Stendahl and D.P. Lew, Leukotriene B4 stimulation of phagocytes results in the formation of inositol 1,4,5-trisphosphate. A second messenger for Ca2+ mobilization, Biochem J,240: 333, (1986)
R.I. Fonteriz, A. Sanchez, F. Mollinedo, E.D. Collado and S.J. Garcia, The role of intracellular acidification in calcium mobilization in human neutrophils, Biochim Biophys Acta,1093:1,(1991)
J. Palmblad, R. Lerner and S.H. Larsson, Signal transduction mechanisms for leukotriene B4 induced hyperadhesiveness of endothelial cells for neutrophils, J Immunol,152: 262, (1994)
T. Yokomizo, T. Izumi, K. Chang, Y. Takuwa and T. Shimizu, A G-protein coupled receptor for leukotriene B4 that mediates chemotaxis, Nature,387: 620, (1997)
C. Owman, C. Nilsson and S.J. Lolait, Cloning of cDNA encoding a putative chemoattractant receptor, Genomics,37: 187, (1996)
S. Narumiya, Structures, properties and distributions of prostanoid receptors., Adv Prostaglandin Thromboxane Leukot Res,23: 17, (1995)
H. Sumimoto and S. Minakami, Oxidation of 20-hydroxyleukotriene B4 to 20-carboxyleukotriene B4 by human neutrophil microsomes. Role of aldehyde dehydrogenase and leukotriene B4 omega-hydroxylase (cytochrome P-450LTB omega) in leukotriene B4 omega-oxidation, J Biol Chem,265: 4348, (1990)
T. Yokomizo, T. Izumi, T. Takahashi, T. Kasama, Y. Kobayashi, F. Sato, Y. Taketani and T. Shimizu, Enzymatic inactivation of leukotriene B4 by a novel enzyme found in the porcine kidney. Purification and properties of leukotriene B4 12-hydroxydehydrogenase, J Biol Chem,268: 18128, (1993)
Z. Honda, T. Takano, Y. Gotoh, E. Nishida, K. Ito and T. Shimizu, Transfected platelet-activating factor receptor activates mitogen-activated protein (MAP) kinase and MAP kinase kinase in Chinese hamster ovary cells, J Biol Chem,269: 2307, (1994)
P.R. Devchand, H. Keller, J.M. Peters, M. Vazquez, F.J. Gonzalez and W. Wahli, The PPARalpha-leukotriene B4 pathway to inflammation control, Nature,384: 39, (1996)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Science+Business Media New York
About this chapter
Cite this chapter
Izumi, T., Yokomizo, T., Igarashi, T., Shimizu, T. (1999). Molecular Cloning and Characterization of Leukotriene B4 Receptor. In: Honn, K.V., Marnett, L.J., Nigam, S., Dennis, E.A. (eds) Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury, 4. Advances in Experimental Medicine and Biology, vol 469. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4793-8_35
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4793-8_35
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7171-7
Online ISBN: 978-1-4615-4793-8
eBook Packages: Springer Book Archive