Fatty Acid Cyclooxygenase Induction and Prostaglandin D Synthesis in a Human Megakaryoblastic Cell Line CMK Differentiated by Phorbol Ester

  • Shozo Yamamoto
  • Natsuo Ueda
  • Ishtiaq Mahmud
  • Hiroko Yamaguchi
  • Rieko Yamashita
  • Kei Yamamoto
  • Kazunori Ishimura
  • Yoshihiro Urade
  • Yoshihide Kanaoka
  • Osamu Hayaishi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 469)

Abstract

The biosynthesis of prostaglandin (PG) and thromboxane (TX) is initiated by a bifunctional enzyme with oxygenase and peroxidase activities, and PGH2 is produced from arachidonic acid via PGG21. This bifunctional enzyme (prostaglandin endoperoxide synthase, E.C. 1.14.99.1) will be referred to briefly as cyclooxygenase in this chapter. There are two cyclooxygenase isozymes (COX-1 and COX-2), and COX-1 is generally known to be present in human platelets and involved in the synthesis of proaggregatory and vasoconstrictive TXA2. Since platelets have no nucleus and can not synthesize mRNA, the COX-1 protein is derived presumably from megakaryocytes which are precursor cells of platelets.

Keywords

Leukemia Glutathione Cysteine Dimethyl Prostaglandin 

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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Shozo Yamamoto
    • 1
  • Natsuo Ueda
    • 1
  • Ishtiaq Mahmud
    • 1
  • Hiroko Yamaguchi
    • 1
  • Rieko Yamashita
    • 1
  • Kei Yamamoto
    • 1
  • Kazunori Ishimura
    • 2
  • Yoshihiro Urade
    • 3
  • Yoshihide Kanaoka
    • 3
  • Osamu Hayaishi
    • 3
  1. 1.Departments of BiochemistryTokushima University, School of MedicineTokushimaJapan
  2. 2.Departments of AnatomyTokushima University, School of MedicineTokushimaJapan
  3. 3.Osaka Bioscience InstituteOsakaJapan

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