Abstract
Recently, it was shown that phospholipase A2 (PLA2) is essential for exposing Mac-1 (CDllb/CD18) on the cell surface of leukocytes1,2. Mac-1 is mandatory for leukocyte migration in vitro, and lack of Mac-1 on human leukocytes is associated with failure of in vitro chemotaxis3. To further substantiate the contribution of PLA2 in cell migration we investigated the effects of PLA2 inhibition on chemotaxis of mixed human leukocytes (MHL) induced by fMLP and the G-protein activator NaF7–9. Migration was measured in a modified 48-well micro-chemotaxis Boyden-chamber6. As PLA2 inhibitors manoalide4, mepacrine5, and BM 16.2115, a new PLA2 inhibitor, were employed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Refferences
Jacobson, P.B., Schlier, D.J., 1993, Regulation of CDllb/CD18 expression in human neutrophils by phospholipase A2. Immunol.151:5639–5652.
Amandi-Burgermeister, E., Tibes, U., Kaiser, B.M., Friebe, W.G., Scheuer, W., 1997, Suppression of cytokine synthesis, integrin expression and chronic inflammation by inhibitors of cytosolic phospholipase A2. Europ. J. Pharmacol.326:237–250.
Arnaout, M.A., 1990, Leukocyte adhesion molecules deficiency: Its structural basis, pathophysiology and implications for modulating the inflammatory response. Immunol. Rev.114:145–180.
Glaser, K.B., Mobilio, D., Chang, J.Y., Senko, N., 1993, Phospholipase A2 enzymes: Regulation and inhibition. Trends Pharmacol. Sci.14:92–98.
Rao, G.N., Lassegue, B., Griendling, K.K., Alexander, R.W., Berk, B.C., 1993, Hydrogen peroxide-induced c-fos expression is mediated by arachidonic acid release. Role of protein kinase C. Nucleic Acids Res.21:1259–1263.
Falk, W., Goodwin, R.H., Leonard, E.J., 1980, A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration. Immunol. Methods33:239–247.
Kadiri, C., Cherqui, G., Masliah, J., Rybkine, T., Etienne, J., Béréziac, G., 1990, Mechanism of N-formyl-methionyl-leucyl-phenylalanine and platelet-activating factor-induced arachidonic acid release in guinea pig alveolar macrophages: Involvement of a GTP-binding protein and role of protein kinase A and protein kinase C. Mol. Pharmacol.38:418–425.
Brom, C., Brom, J., König, W., 1991, G-protein activation and mediator release from human neutrophils and platelets after stimulation with sodium fluoride and receptor-mediated stimuli. Immunology73:287–292.
Kozawa, O., Tokuda, H., Miwa, M., Takahashi, Y., Ozaki, N., Oiso, Y., 1992, Mechanism of prostaglandin E2-induced arachidonic acid release in osteoblast-like cells: Independence from phosphoinositide hydrolysis. Prostaglandins Leukot. Essent. Fatty Acids46:291–295.
Zigmond, S.H., Hirsch, J.G., 1973, Leukocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. Exp. Med.137:387–410.
Samuelsson, B., Dahlen, S.E., Lindgren, J.A., Rouzer, C.A., Serhan, C.N., 1987, Leukotrienes and lipoxins: Structures, biosynthesis, and biological effects. Science237:1171–1176.
Ford-Hutchinson, A., Bray, W.M., Doig, M.V., Shipley, M.E., Smith, M.J.H., 1980, Leukotriene B4 a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytes. Nature286:264–265.
Gierschik, P., Camps, M., 1994, Stimulation of phospholipase C by G-protein βγ subunits. Methods in Enzymology 238:181–195.
Vondran, A., Tibes, U., Borowski, E., Friebe, W.G., Scheuer, W.V., 1997, New type II phospholipase A2 inhibitors effective in blocking an ongoing enzyme reaction: Inability of manoalide and scalaradial to exert an inhibitory capacity. In: Phospholipase A2. Basic and Clinical Aspects in Inflammmatory Diseases. Eds Uhl, W., Nevalainen, T.J., Büchler, M.W., Progress in Surgery 24: 130–139. Karger, Basel.
Tibes, U., Scheuer, W.V., Thierse, H.J., Burgermeister, E., Schramm, S., Friebe, W.G., Dietz, E., 1997, Role of cytosolic PLA2, secretory PLA2 and nitric oxide synthase in inflammation. In: Phospholipase A2. Basic and Clinical Aspects in Inflammmatory Diseases. Eds Uhl, W., Nevalainen, T.J., BücMer, M.W., Progress in Surgery24:153–167. Karger, Basel.
Pfeilschifter, J., Schalkwijk, C.G., Briner, V.A., van-den-Bosch, H., 1993, Cytokine-stimulated secretion of group II phospholipase A2 by rat mesangial cells. Its contribution to arachidonic acid release and prostaglandin synthesis by cultured rat glomerular cells. J. Clin. Invest. 92:2516–2523.
Grossman, C.J., Wiseman, J., Lucas, F.S., Trevethick, M.A., Birch, P.J., 1995, Inhibition of constitutive and inducible cyclooxygenase activity in human platelets and mononuclear cells by NSAIDs and Cox 2 inhibitors. Inflamm.Res.44:253–257.
Tibes, U., Vondran, A., Rodewald, E., Friebe, W.G., Schäfer, W., Scheuer, W., 1995, Inhibition of allergic and non-allergic inflammation by phospholipase A2 inhibitors. Int Arch Allergy Immunol 107:432–434.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1999 Springer Science+Business Media New York
About this chapter
Cite this chapter
Tibes, U., Hinder, M., Scheuer, W., Friebe, WG., Schramm, S., Kaiser, B. (1999). Phospholipase A2 is Involved in Chemotaxis Of Human Leukocytes. In: Honn, K.V., Marnett, L.J., Nigam, S., Dennis, E.A. (eds) Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation, and Radiation Injury, 4. Advances in Experimental Medicine and Biology, vol 469. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4793-8_29
Download citation
DOI: https://doi.org/10.1007/978-1-4615-4793-8_29
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-7171-7
Online ISBN: 978-1-4615-4793-8
eBook Packages: Springer Book Archive