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α-Gal Epitopes in Animal Tissue Glycoproteins and Glycolipids

  • Lennart Rydberg
  • Jan Holgersson
  • Bo E. Samuelsson
  • Michael E. Breimer
Chapter
Part of the Subcellular Biochemistry book series (SCBI, volume 32)

Abstract

The great structural complexity of carbohydrate chains originates in the many ways the monosacchrides can be linked to each other. Binding position (e.g., 1,3 as opposed to 1,4), binding anomericity (i.e., a or β), monosaccharide structure (e.g., pentose vs hexose), ring size (i.e., furanose vs pyranose), and stereochemistry all contribute to structural complexity. Furthermore, the carbohydrate chain may be branched or non-branched and the monosaccharide units may be chemically modified through, for example sulfation or phosphorylation. For each linkage established between two monosaccharides, there is an enzyme, a glycosyltransferase, catalyzing the elongation of the growing carbohydrate chain (Natsuka and Lowe, 1994). Sometimes, there are even different glycosyltransferases catalyzing the ases catalyzing the formation of the same linkage. Carbohydrate antigens are therefore “secondary” gene products as they are produced by the joint action of a series of “primary” gene products, the glycosyltransferases. The structural diversity found in the primary sequence of a few monosaccharides is immense compared to the relatively limited structural variability obtained in the primary sequence of the same number of amino acids. If three different amino acids are combined in every possible way, six different polypeptide structures are obtained. If the same theoretical calculation is done with three different monosaccharides, one ends up with more than 1.000 different trisaccharide structures (Samuelsson and Breimer, 1987). However, nature does not, to our knowledge, make use of all these possible structures.

Keywords

Blood Group Sugar Residue Carbohydrate Chain Rabbit Erythrocyte Porcine Aortic Endothelial Cell 
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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Lennart Rydberg
    • 1
  • Jan Holgersson
    • 2
  • Bo E. Samuelsson
    • 1
  • Michael E. Breimer
    • 3
  1. 1.Institute of Laboratory Medicine, Department of Clinical Chemistry and Transfusion MedicineSahlgrenska universitetssjukhusetSweden
  2. 2.Division of Clinical Immunology, Karolinska InstituteHuddinge University HospitalHuddingeSweden
  3. 3.Institute for Surgical Sciences, Department of SurgeryUniversity of Göteborg, Sahlgrenska universitetssjukhusetGöteborgSweden

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