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Protoplast Fusion in Disease Control

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Abstract

The use of ecologically safe and environmentally friendly methods of protecting crops from pests and pathogens is gaining importance. Control methods using chemical pesticides may lead to development of resistant pathogenic strains, moreover, their accumulation can cause serious ecological and health problems. Therefore, the control of plant diseases by the use of existing organism in their natural habitat is an urgently needed component of plant disease management. Biocontrol of plant diseases offers answers to many persistant problems in agriculture including problems of resource limitation, non-sustainable agriculture system and over-reliance on pesticides (Cook and Baker, 1983). Biocontrol agents have potential to replace chemical pesticides (Mukerji and Garg, 1988 a, b). Moreover, they offer advantages in plant health management not possible with chemical pesticides. Despite these advantages, beneficial microbes are rarely used to control plant diseases primarly because biocontrol agents have been both less effective and more variable than competitive pesticides. A principal reason for this is the poor growth of bioprotectant. The ability of bioprotectant to proliferate in a competitive microenvironment is crucial.

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References

  • Adams, G., Johnson, N., Leslie, J.F. and Hart, L.P. 1987, Heterokaryons of Gibberella zeae formed following hyphal anastomosis or protoplast fusion, Exp. Mycol. 11: 339–353.

    Article  Google Scholar 

  • Ahmad, J.S. and Baker, R. 1987, Rhizosphere competence of Trichoderma harzianum, Phytopathology 77: 182–189.

    Article  Google Scholar 

  • Andrade, C.M.M., Oliveira, F.M. and Valter, R.L. 1992, Candida fennica: enhancement of protoplast formation and fusion, Can. J. Microbiol. 38: 807–810.

    Article  CAS  Google Scholar 

  • Anne, J., and Peberdy, J.F. 1975, Conditions for induced fusion of fungal protoplasts in polyethylene glycol solutions, Arch. Microbiol. 105: 201–205.

    Article  PubMed  CAS  Google Scholar 

  • Anne, J., and Peberdy, J.F. 1976, Induced fusion of fungal protoplasts following treatment with polyethylene glycol, J. Gen. Microbiol. 92: 413–417.

    Article  PubMed  CAS  Google Scholar 

  • Anne, J., Eyssen, H. and de Somer, P. 1974, Formation and regeneration of Penicillium chrysogenum protoplasts, Arch. Microbiol. 98: 159–166.

    Article  PubMed  CAS  Google Scholar 

  • Benitez, T., Ramos, S. and Garcia Acha, I. 1975a,Protoplasts of Trichoderma viride: formation and regeneration, Arch. Microbiol. 103: 199–203.

    CAS  Google Scholar 

  • Benitez, T., Villa, T.G. and Garcia Acha, I. 1975b,Chemical and structural differences in mycelial and regeneration walls of Trichoderma viride, Arch. Microbiol, 105: 277–282.

    Article  PubMed  CAS  Google Scholar 

  • Benkhemmar, O., Gaudemer, F. and Bouvier-Fourcade, I. 1985, Heterokaryosis between Aspergillus oryzae cyclopiazonic acid-defective strains: method for estimating the risk of inducing toxin production among cyclopiazonic acid-defective industrial strains, App. Environ. Microbiol. 50: 1087–1093.

    CAS  Google Scholar 

  • Bloomfield, B.J. and Alexander, M. 1967, Melanins and resistance of fungi to lysis, J. Bacteriol. 93: 1267–1280.

    Google Scholar 

  • Bojnanska, A., Sipiczki, M. and Ferenczy, L. 1980, Characterization of conidiation mutants in Trichoderma viride by hyphal anastomosis and protoplast fusion, Acta. Microbiol. Acad. Sci. Hung. 27: 305–307.

    PubMed  CAS  Google Scholar 

  • Bok, J., Kim, J., Jin, M., Choi, E. and Kim, B. 1994, Studies on protoplast formation and regeneration of Lyophyllum decastes, Korean J. Mycol. 22: 130–137.

    CAS  Google Scholar 

  • Bok, J., Park, S., Chol, E., Kim, B. and Yoo, Y. 1990, Studies on protoplast formation and regeneration of Coriolus versicolor, Korean J. Mycol. 18: 115–126.

    Google Scholar 

  • Bradshaw, R.E., Lee, K. and Peberdy, J.F. 1983, Aspects of genetic interaction in hybrids of Aspergillus nidulans and Aspergillus rugulosus obtained by protoplast fusion, J. Gen. Microbiol. 129: 3525–3533.

    PubMed  CAS  Google Scholar 

  • Bu’lock, J.D., Wright, C.E. and Mooney, J.E. 1986, Use of a protoplast fusion test to establish the status of mycotohin genes in an edible Fusarium, Biotechnol. Lett 8: 621–624.

    Article  CAS  Google Scholar 

  • Bull, A.T., 1970, Chemical composition of wild type and mutant Aspergillus nidulans cell walls: the nature of the polysaccharide and melanin constituents, J. Gen. Microbiol. 63:75–94.

    Article  PubMed  CAS  Google Scholar 

  • Cary, J.W. and Stovall, M.E. 1992, Optimum conditions forrelease and regeneration of protoplasts of Alternaria alternata, Lett. Appl. Microbiol. 14: 100–103.

    Article  Google Scholar 

  • Chadegani, M., and Ahmadjian, V., 1991, Protoplast fusion between mutant strains of Microsporum gypseum, Mycologia 83: 779–786.

    Article  Google Scholar 

  • Chadegani, M., Brink, J.J., Shehata, A. and Ahmadjian, V. 1989, Optimization of protopast formation, regeneration and viability in Microsporum gypseum, Mycopathologia 107: 33–50.

    Article  PubMed  CAS  Google Scholar 

  • Chang, L.T., Terasaka, D.T. and Elander, R.P. 1981, Protoplast fusion in industrial fungi, Dev. Ind. Microbiol. 23: 21–29.

    Google Scholar 

  • Chang, S.Y., Li, C.T., Hiang, S.Y. and Chang, M.C. 1995, Intraspecific protoplast fusion of Candida tropicalis for enhancing phenol degradation, Appl. Microbiol. Biotechnol. 43: 534–538.

    Article  CAS  Google Scholar 

  • Choi, S.H., Sung, C., Oh, M.J. and Kim, C.J. 1997, Intergeneric protoplast fusion in Saccharomycopsis fibuligera and Saccharomyces cerevisiae, J. Ferement. Bioengg. 84:156–161.

    Google Scholar 

  • Chung, H., Him, S. and Ahm, H. 1992, Protoplast formation and fusion between anastomosis groups of Rhizoctonia solani, Korean J. Mycol. 20: 44–50.

    CAS  Google Scholar 

  • Cook, R.J. and Baker, K.F. 1983, The Nature and Practice of Biological Control of Plant Pathogens, 2nd ed., American Phytopathological Society, St. Paul, Minnesota, USA.

    Google Scholar 

  • Cooley, R.N., Shaw, R.K., Frankli, F.C.H. and Caten, C.E. 1988, Transformation of the phytopathogenic fungus Septoria nodorum to hygromycin resistance, Curr. Genet. 13: 383–389.

    Article  CAS  Google Scholar 

  • Constable, F. and Kao, K.N. 1974, Agglutination and fusion of plant protoplasts by polyethylene glycol, Can. J. Bot. 52: 1603–1606.

    Article  Google Scholar 

  • Couteaudier, Y., Viaud, M. and Riba, G. 1996, Genetic nature, stability, and improved virulence of hybrids from protoplast fusion in Beauveria, Microbial Eco. 32: 1–10.

    Google Scholar 

  • Davis, B., d’Avillez Paixao, M.T., Deans, S.G. and Smith, J.E. 1977, Protoplast formation from giant cells of Aspergillus niger, Trans. Br. Mycol. Soc. 69: 207–212.

    Article  Google Scholar 

  • de Figueroa, L.I., de Richard, M.F. and de van Broock, M.R. 1984, Interspecific protoplast fusion of the baker’s yeast Saccharomyces cerevisiae and Saccharomyces diastaticus, Biotechnol. Lett 6: 269–274.

    Article  Google Scholar 

  • de Vires, O.M.H. and Wessels, J.G.H. 1972, Release of protoplasts from Schizophyllum commune by a lytic enzyme preparation from Trichoderma viride, J. Gen. Microbiol. 73: 13–22.

    Article  Google Scholar 

  • de Vries, O.M.H. and Wessels, J.G.H. 1975, Chemical analysis of cell wall regeneration and reversion of protoplasts from Schizophyllum commune, Arch. Microbiol. 102: 209–218.

    Article  PubMed  Google Scholar 

  • Deutch, C.E. and Parry, J.M. 1974, Sphaeroplast formation in yeast during the transition from exponential phase to stationary phase, J. Gen. Microbiol. 80: 259–268.

    Article  Google Scholar 

  • Dhawale, M.R. and Ingledew, W.M. 1983, Interspecific protoplast fusion of Schwanniomyces yeasts, Biotechnol. Lett. 5: 825–830.

    Article  CAS  Google Scholar 

  • Dias, E.S., Araujo, E.F., Guimaraes, W.V, Coelho, J.L.C., and Silva, D.O. 1997, Production and generation of Penicillium expansum and Penicillium grisoroseum protoplasts, Revista de MicrobioL 28: 116–120.

    Google Scholar 

  • Didek-Brumec, M., Gaberc-Porekar, V., Alacevic, M. and Socic, H. 1993, Strain improvement of Claviceps purpurea by protoplast fusion without introducing auxotrophic markers, Appl. Microbiol. Biotechnol. 38: 746–749.

    Article  CAS  Google Scholar 

  • Dlugonski, J., Sedlaczek, L. and Jaworski, A. 1984, Protoplast release from fungi capable of steroid transformation, Can. J. Microbiol. 30: 57–62.

    Article  PubMed  CAS  Google Scholar 

  • Dooijewaard-Kloosterziel, A.M.P., Sietsma, J.H. and Wouters, J.T.M. 1973, Formation and regeneration of Geotrichum candidum protoplasts, J. Gen. Microbiol. 74: 205–209.

    Article  PubMed  CAS  Google Scholar 

  • Eidtmann, A. and Schauz, K. 1992, Cryopreservation of protoplasts from sporidia of Ustilago maydis, Mycol. Res. 96:318–320.

    Article  CAS  Google Scholar 

  • Evans, C.T. and Conrad, D. 1987, An improved method for protoplast formation and its application in the fusion of Bhodotorula rubra with Saccharomyces cerevisiae, Arch. Microbiol. 148: 77–82.

    Article  PubMed  CAS  Google Scholar 

  • Farahnak, F., Seki, T., Ryu, D.D.Y. and Ogrydziak, D. 1986, Construction of lactose-assimilating and high-ethanol-producing yeast by protoplast fusion, Appl. Environ. MicrobioL 51: 362–367.

    PubMed  CAS  Google Scholar 

  • Farman, M.L. and Oliver, R.P. 1988, The transformation of protoplasts of Leptosphaeria maculans to hygromycin B resistance, Curr. Genet. 13: 327–330.

    Article  CAS  Google Scholar 

  • Ferenczy, L. 1976, Some characteristics of intra-and intrspecific protoplast fusion products of Aspergillus nidulans and Aspergillus fumigatus, in: Cell Genetics in Higher Plants, D. Dudits, G.L. Farkas and P. Maliga, eds., Akademiai Kiado, Budapest.

    Google Scholar 

  • Ferenczy, L. Kevei, F. and Szegedi, M. 1975a,Increased fusion frequency of Aspergillus nidulans protoplasts, Experimentia 31: 50–52.

    Article  CAS  Google Scholar 

  • Ferenczy, L., Kevei, F. and Szegedi, M. 1975b,High frequency fusion of fungal protoplasts, Experimentia 31: 1028–1030.

    Article  CAS  Google Scholar 

  • Furlaneto, M.C. and Pizzirani-Kleiner, A.A. 1992, Intraspecific hybridization of Trichoderma pseudokoningii by anastomosis and by protoplast fusion, FEMS Microbiol. Lett 90:191–196.

    Article  Google Scholar 

  • Gabriel, M. and Kopecka, M. 1983, Regeneration of the cell walls of protoplast of the fission yeast Schizosaccharomyces versatilis in liquid media and their reversion to cells, Folia Microbiol. 28: 452–457.

    Article  Google Scholar 

  • Gabriel, M., Kopecka, M. and Svoboda, A. 1992, Structural rearrangement of the actin cytoskeleton in regenerating protoplasts of budding yeasts, J. Gen. Microbiol. 138: 2229–2234.

    Article  CAS  Google Scholar 

  • Garcia Acha, I., Lopez-Belmonte, F. and Villanueva, J.R. 1966, Regeneration of mycelial protoplasts of Fusarium culmorum, J. Gen. Microbiol. 45: 515–523.

    Article  CAS  Google Scholar 

  • Gautam, S.P., Gupta, A.K., Shrivastava, R. and Awasthi, M. 1996, Protoplast formation from the thermophilic fungus Malbranchea sulfurea, using the thermostable chitinase and laminarinase of Paecilomyces varioti, World J. Microbiol. Biotechnol. 12: 99–100.

    Article  CAS  Google Scholar 

  • Gold, M.H., Cheng, T.M. and Alic, M. 1983, Formation, fusion and regeneration of protoplasts from wild-type and auxotrophic strains of the white rot basidiomycete Phanerochaeta chrysosporium, Appl. Environ. Microbiol. 46: 260–263.

    PubMed  CAS  Google Scholar 

  • Goldman, G.H., Montagu, M.V. and Herrera-Estrella, A. 1990, Transformation of Trichoderma harzianumby high voltage electric pulse, Curr. Genet 17:169–174.

    Article  CAS  Google Scholar 

  • Goto-Hamamoto, M., Ohnuki, T., Uozumi, T. and Beppu, T. 1986, Intraspecific hybridization by protoplast fusion in Mucorales producing milk-clotting proteases, Agric. Biol. Chem. 50: 1467–1473.

    Article  CAS  Google Scholar 

  • Hader, Y., Harman, G.E. and Taylor, A.G. 1984, Evaluation of Trichoderma koningii and T. harzianum from New York soils for biological control of seed rot caused by Pythium spp., Phytopathology 74: 106–110.

    Article  Google Scholar 

  • Hamlyn, P.F., Birkett, J.A., Perez, G. and Peberdy, J.F. 1985, Protoplast fusionas a tool for genetic analysis in Cephalosporium aceremonium, J. Gen. Microbiol. 131: 2813–2823.

    PubMed  CAS  Google Scholar 

  • Hamlyn, P.F., Bradshaw, R.E., Mellon, F.M., Santiago, C.M., Wilson, J.M. and Peberdy, J.F. 1981, Efficient protoplast isolation from fungi using commercial enzymes, Enz. Microb. Technol. 3: 321–325.

    Article  CAS  Google Scholar 

  • Harman, G.E. 1991, Seed treatment for biological control of plant disease, Crop Protecion 10: 166–171.

    Article  Google Scholar 

  • Harman, G.E. and Stasz, T.E. 1991, Protoplast fusion for the production of superior biocontrol fungi, in: Microbial Control of Weeds, D.O. TeBeest, ed., Chapman and Hall, New York.

    Google Scholar 

  • Harman, G.E. and Taylor, A.G. 1990, Development of an effective biocontrol seed treatment system, in: Biological Control of Soil-Borne Plant Pathogens, D. Hornby, ed., C.A.B. International, Wallingford.

    Google Scholar 

  • Harman, G.E., Taylor, A.G. and Stasz, T.E. 1989, Combining effective strains of Trichoderma harzianum and solid matrix priming to improve biological seed treatment, Plant Dis. 73: 631–637.

    Article  Google Scholar 

  • Hashiba, T. and Yamada, M. 1982, Formation and purification of protoplasts from Rhizoctonia solani, Phytopathology 72: 849–853.

    Article  CAS  Google Scholar 

  • Heluane, H., Spencer, J.F.T., Spencer, D., de Figueroa, L. and Challieri, D.A.S. 1993, Characterization of hybrids obtained by protoplast fusion, between Pachysolen tannophilus and Saccharomyces cerevisiae, Appl. Microbiol. Biotechnol. 40: 98–100.

    Article  CAS  Google Scholar 

  • Hocart, M.J. and McNaughton, J.E. 1994, Interspecific hybridization between Pseudocercosporella herpotrichoides and P. anguioides achieved through protoplast fusion, Mycol. Res. 98: 47–56.

    Article  Google Scholar 

  • Hocart, M.J. and Peberdy, J.F. 1989, Protoplast technology and strain selection, in: Biotechnology of Fungi for Improving Plant Growth, J.M. Whipps and R.D. Lumsden, eds., Cambridge University Press, Cambridge.

    Google Scholar 

  • Hocart, M.J., Lucas, J.A. and Peberdy, J.F. 1987, Production and regeneration of protoplasts from Pseudocercosporella herpotrichoides (Fron) Deighton, J. Phytopathology 119: 193–205.

    Article  CAS  Google Scholar 

  • Hocart M.J., Lucas, J.A. and Peberdy, J.F. 1993, Parasexual recombination between W and R pathotypes of Pseudocercosporella herpotrichoides through protoplast fusion, Mycol. Res. 97: 977–983.

    Article  Google Scholar 

  • Homolka, L. 1988, Preparation reversion, mutagenesis and intra-species fusion of protoplasts of Oudemansiella mucida, Folia Microbiol. 33: 298–308.

    Article  CAS  Google Scholar 

  • Housset, P., Nagy, M. and Schwencke, J. 1975, Protoplasts of Schizosaccharomyces pombe: an improved method for their preparation and the study of their guanine uptake, J. Gen. Microbiol. 90: 260–264.

    Article  PubMed  CAS  Google Scholar 

  • Huang, D., Staples, R.C., Bushnell, W.R. and Maclean, D.J. 1990, Preparation and regeneration of protoplasts from axenic mycelia derived from the wheat stem rust fungus, Phytopathology 80: 81–84.

    Article  Google Scholar 

  • Jackson, C.W. and Heale, J.B. 1987, Parasexual crosses by hyphal anastomosis and protoplast fusion in the entomopathogen Vertilcillium lecani. J. Gen. Microbiol. 133: 3537–3547.

    Google Scholar 

  • Kavanagh, K. and Whittaker, P.A. 1991, Effect of buffer viscosity on the rate of protoplast formation in yeast, Biomedical Lett. 46: 57–62.

    Google Scholar 

  • Kavanagh, K., Walsh, M. and Whittaker, P.A. 1991, Enhanced intraspecific protoplast fusion in yeast, FEMS Microbiol. Lett 81: 283–286.

    Article  CAS  Google Scholar 

  • Kevei, F. and Peberdy, J.F. 1984, Further studies on protoplast fusion and interspecific hybridization within the Aspergillus nidulans group, J. Gea Microbiol. 130: 2229–2236.

    CAS  Google Scholar 

  • Kirimura, K., Imura, M., Lee, S.P., Kato, Y. and Usami, S. 1989, Intergeneric hybridization between Aspergillus niger and Trichoderma viride by protoplast fusion, Agric. Biol. Chem. 53: 1589–1596.

    Article  Google Scholar 

  • Kirimura, K., Sato, T., Nakanishi, N., Terada, M. and Usami, S. 1997, Breeding of starch utilizing and itaconic-acid-producing kojimolds by interspecific protoplast fusion between Aspergillus terreus and Aspergillus usamii, Appl. Microbiol. Biotechnol. 47: 127–131.

    Article  CAS  Google Scholar 

  • Kitamoto, Y., Mori, N., Yamamoto, M., Ohiwa, T. and Ichikawa, Y. 1988, A simple method for protoplast formation and improvement of protoplast regeneration from various fungi using an enzyme from Trichoderma harzianum, Appl. Microbiol. Biotechnol. 28: 445–450.

    Article  CAS  Google Scholar 

  • Kobayashi, I., Ohkuma, T., Ishizaki, H. and Kunoh, H. 1985, Hardness of culture media affecting regeneration and reversion of Pyricularia oryzae protoplasts and a thin-layer agar-plate method devised for numerical evaluation of regeneration and reversion, Exp. Mycol. 9:161–169.

    Article  Google Scholar 

  • Kobori, H., Takata, Y. and Osumi, M. 1991, Interspecific protoplast fusion between Candida tropicalis and Candida boidinii: characterization of the fusants, J. Ferment. Bioengg. 72: 439–444.

    Article  CAS  Google Scholar 

  • Kobori, H., Yamada, N., Taki, A. and Osumi, M. 1989, Actin is associated with the formation of cell wall in reverting protoplasts of the fission yeast Schizosaccharomyces pombe, J. Cell Sci. 94: 635–646.

    PubMed  Google Scholar 

  • Kopecka, M. and Gabriel, M. 1995, Actin cortical cytoskeleton and cell wall synthesis in regenerating protoplasts of the Saccharomyces cerevisiae actin mutant DBY 1693, Microbiology 141: 1289–1299.

    Article  PubMed  CAS  Google Scholar 

  • Kovac, L. and Subik, J. 1970, Some aspects of the preparation of yeast protoplasts and isolation of mitochondria, Acta. Fac. Med. Univ. Brun. 37: 91–94.

    Google Scholar 

  • Kucsera, J. and Ferenczy, L. 1986, Interspecific hybridization between Candida albicans and Candida tropicalis, FEMS Microbiol. Lett 36:315–318.

    Article  CAS  Google Scholar 

  • Kumar, P.K.R. and Lansane, B.K. 1988, Kinetics of mycelial protoplast formation in Gibberella fujikuroi and Trichoderma reesei, Folia MicrobioL 33: 183–187.

    Article  CAS  Google Scholar 

  • Kumari, J.A. and Panda, T. 1992, Studies on critical analysis of factors influencing improved production of protoplasts from Trichoderma reesei mycelium, Enz. Microb. Technol. 14:241–248.

    Article  CAS  Google Scholar 

  • Lalithakumari, D., Mrinalini, C., Chandra, A.B. and Annamalai, P. 1996, Strain improvement by protoplast fusion for enhancement of biocontrol potential integrated with fungicide tolerance in Trichoderma spp., Zeitschrift fur Pflanzenkrankeiten und Pflanzenschutz 103: 206–212.

    Google Scholar 

  • Law, C., Kavanagh, K. and Whittaker, P. 1994, Analysis of hybrids of Candida albicans formed by protoplast fusion, FEMS Microbiol. Lett 115:77–82.

    Article  PubMed  CAS  Google Scholar 

  • Layton, A.C. and Kuhn, D.N. 1988, Heterokaryon formation by protoplast fusion of drug-resistant mutants in Phytophthora megasperma f.sp. glycinea, Exp. Mycol. 12: 180–194.

    Article  Google Scholar 

  • Lynch, P.T., Collin, H.A. and Issac, S. 1985a,Use of autolytic enzyme for isolation of protoplasts from Fusarium tricinctum hyphae, Trans. Br. Mycol. Soc. 84: 473–478.

    Article  CAS  Google Scholar 

  • Lynch, P.T., Collin, H.A and Issac, S. 1985b,Isolation and regeneration of protoplasts from Fusarium tricinctum and F.oxysporum, Trans.Br. Mycol. Soc. 85: 135–140.

    Article  Google Scholar 

  • Lynch, P.T., Issac, S. and Collin, H.A. 1989, Electrofusion of protoplasts from Aspergillus nidulans, FEMS Microbiol. Lett 59: 225–228.

    Article  Google Scholar 

  • Maliszewsk, I.H. and Zboinska, E.M. 1996, Penicillium citrinum protoplasts: preparatori, regeneration and lipolytic activity of regenerants, Acta Biotech. 16: 193–198.

    Article  Google Scholar 

  • Manczinger, L. and Ferenczy, L. 1985, Somatic cell fusion of Trichoderma reesei resulting in new genetic combinations, Appl. Microbiol. Biotechnol. 22: 72–76.

    Article  CAS  Google Scholar 

  • Manczinger, L., Antal, Z. and Ferenczy, L. 1995, Isolation of uracil auxotrophic mutants of Trichoderma harzianum and their transformation with heterologus vectors, FEMS Microbiol. Lett 130: 59–62.

    CAS  Google Scholar 

  • Maraz, A. and Subik, J. 1981, Transmission and recombination of mitochondrial genes in Saccharomyces cerevisiae after protoplast fusion, Mole.Gen. Genet 181: 131–133.

    Article  CAS  Google Scholar 

  • Migheli, Q., Whipps, J.M., Budge, S.P. and Lynch, J.M. 1995, Production of inter-and intra-strain hybrids of Trichoderma spp. by protoplast fusion and evaluation of their biocontrol activity against soil-borne and foliar pathogens, J. Phytopathology 143: 91–97.

    Article  Google Scholar 

  • Mizuno, K., Kimura, O. and Tachiki, T. 1997, Protoplast formation from Schizophyllum commune by a culture filterate of Bacillus circulons KA-304 grown on cell-wall preparations of S. commune as a carbon source, Biosci. Biotech. Biochem. 61:852–857.

    Article  CAS  Google Scholar 

  • Morinaga, T., Kikuchi, M. and Nomi, R. 1985, Formation and regeneration of protoplasts in Coprinus pellucidus and Coprinus cinereus, Agric. Biol. Chem. 49: 523–524.

    Article  CAS  Google Scholar 

  • Mukerji, K.G. and Garg, K.L. 1988a, Biocontrol of Plant Diseases, Vol. I, CRC Press, BocaRaton, Florida.

    Google Scholar 

  • Mukerji, K.G. and Garg, K.L. 1988b, Biocontrol of Plant Diseases, Vol. II, CRC Press, BocaRaton, Florida.

    Google Scholar 

  • Musilkova, M., Fencil, Z. and Seichertova, O. 1969, Release of Aspergillus niger protoplasts by Penicillium purpurogenum enzymes, Folia Microbiol. 14: 47–50

    Article  CAS  Google Scholar 

  • Nagy, A., Vagvolgyi, Cs., Balla, E. and Ferenczy, L. 1994, Electrophoretic karyotype of Mucor circinelloides, Curr. Genet. 26: 45–48.

    Article  PubMed  CAS  Google Scholar 

  • Nga, B.H., Abu Baker, F.D., Loh, G.H., Chiu, L.L., Harashima, S., Oshima, Y. and Heslot H. 1992, Integeneric hybrids between Saccharomycopsis flbuligera and Yarrowia lipolytica, J. Gen. Microbiol. 138: 223–227.

    Article  Google Scholar 

  • Oberma, H., Stobinska, H. and Kregiel, D. 1990, Obtaining and regeneration of Trichosporon cutaneum TrNul8 protoplasts, Acta Aliment Pol. 16: 83–96.

    Google Scholar 

  • Ogawa, K., Brown, J.A. and Wood, T.M. 1987, Intraspecific hybridization of Trichoderma reesei QM 9414 by protoplast fusion using colour mutants, Enz. Microb. Technol. 9: 229–232.

    Article  CAS  Google Scholar 

  • Ogawa, K., Ohara, H. and Toyama, N. 1988, Intraspecific hybridization of Aspergillus awamori var. kawachi by protoplast fusion, Agric. Biol. Chem. 52: 337–342.

    Article  CAS  Google Scholar 

  • Ogawa, K., Tsuchimochi, M., Taniguchi, K. and Nakatsu, S. 1989, Interspecific hybridization of Aspergillus usamii mut shirousamii and Aspergillus niger by protoplast fusion, Agric. Biol. Chem. 53: 2873–2880.

    Article  CAS  Google Scholar 

  • Ohnuki, T., Etoh, Y. and Beppu, T. 1982, Intraspecific and interspecific hybridization of Mucor pusillus and M. miehei by protoplast fusion, Agric. Biol. Chem. 46: 451–458.

    Article  CAS  Google Scholar 

  • Peberdy, J.F. 1976, Isolation and properties of protoplasts from filamentous fungi, in: Microbial and Plant Protoplasts, J.F. Peberdy, A.H. Rose, H.J. Rogers and E.C. Cocking, eds., Academic Press, London.

    Google Scholar 

  • Peberdy, J.F. 1979, Fungal protoplasts: isolation, reversion and fusion, Ann. Rev. Microbiol. 33: 21–39.

    Article  CAS  Google Scholar 

  • Peberdy, J.F. and Gibson, R.K. 1971, Regeneration of Aspergillus nidulans protoplasts, J. Gen. Microbiol. 69: 325–330.

    Article  PubMed  CAS  Google Scholar 

  • Pe’er, S. and Chet, I. 1990, Trichoderma protoplast fusion: a tool for improving biocontrol agents, Can. J. Microbiol. 36: 6–9.

    Article  Google Scholar 

  • Peng, M., Lemke, P.A. and Shaw, J.J. 1993, Improved conditions for protoplast formation and transformation of Pleurotos ostreatus, Appl. Microbiol. Biotechnol. 40: 101–106.

    Article  CAS  Google Scholar 

  • Pesti, M. and Ferenczy, L. 1982, Protoplast fusion hybrids of Candida albicans sterol mutant differing in nystatin resistance, J. Gen. Microbiol. 128:123–128.

    PubMed  CAS  Google Scholar 

  • Picataggio, S.K., Schamhart, D.M.J., Montenecourt, B.S. and Eveleigh, D.E. 1983, Sphaeroplast formation and regeneration in Trichoderma reesei, Eur. J. Appl. Microbiol. Biotechol. 17: 121–128.

    Article  Google Scholar 

  • Pina, A. Calderon, I.L., and Benitez, T. 1986, Integeneric hybrids of Saccharomyces cerevisiae and Zygosaccharomyces fermentati obtained by protoplast fusion, Appl. Environ. Microbiol. 51: 995–1003.

    PubMed  CAS  Google Scholar 

  • Quigley, D.R., Taft, C.S., Stark, T. and Selitrennikoff, C.P. 1987, Optimal conditions for the release of protoplasts of Neurospora using Novozym 234, Exp. Mycol. 11:236–240.

    Article  CAS  Google Scholar 

  • Reyes, F., Perez-Leblic, M.I., Martinez, M.J. and Lahoz, R. 1984, Protoplast production from filamentous fungi with their own autolytic enzymes, FEMS Microbiol. Lett. 24: 281–283.

    Article  CAS  Google Scholar 

  • Richards, W.C. 1994, An enzyme system to liberate spore and mycelial protoplasts from a dimorphic fungal plant pathogen Ophiostoma ulmi (Buism.) Nannf, Physiol. Mole. Plant Pathol. 44:311–319.

    Article  CAS  Google Scholar 

  • Rlike, O., Baum, A., Weiss, J., Hommel, R. and Kleber, H.P. 1992, Kinetics of enzymatic lysis, formation and regeneration of protoplasts of Candida (Torulopsis) apicola, World J. Microbiol. Biotechnol. 8: 14–20.

    Article  Google Scholar 

  • Sakai, T., Koo, K., Saitoh, K. and Katsuragi, T. 1986, Use of protoplast fusion for the development of rapid starch fermenting strains of Saccharomyces diastaticus, Agric. Biol. Chem. 50: 297–306.

    Article  CAS  Google Scholar 

  • Sandhu, D.K., Bawa, S. and Bagga, P.S. 1989, Optimization of protoplast production from Trichoderma harzianum, Ind. J. Exp. Biol. 27: 180–181.

    Google Scholar 

  • Santos, T.M.C., and DeMelo, I.S. 1991, Preparation and regeneration of protoplasts of Talaromyces flavus, Rev. Bras. Genet 14: 835–840.

    Google Scholar 

  • Sarachek, A., Rhoads, D.D. and Schwarzhoff, R.H. 1981, Hybridization of Candida albicans through fusion of protoplasts, Arch. Mcrobiol. 129: 1–8.

    Article  CAS  Google Scholar 

  • Sato, M., Toya, M., Shimura, Y., Kobori, H. and Osumi, M. 1993, Ultrastructural changes during the interspecific protoplast fusion between Candida tropicalis and Candida boidinii observed by TEM, Plant Morphology 5: 117–126.

    Article  Google Scholar 

  • Shabana, Y.M. and Charudattan, R. 1997, Preparation and regeneration of mycelial protoplasts of Alternaria eichhorniae, J. Phytopathology 145: 335–338.

    Article  CAS  Google Scholar 

  • Shin, P. and Cho, M. 1994, Intergeneric protoplast fusion between Gliocladium virens and Trichoderma harzianum, Korean. J. Mycol. 21: 323–331.

    Google Scholar 

  • Sienko, M., Stepien, P.P. and Paszewski, A. 1992, Generation of genetic recombinants in Trichosporon cutaneum by spontaneous segregation of protoplast fusants, J. Gen. Microbiol. 138: 1409–1412.

    Article  PubMed  CAS  Google Scholar 

  • Sietsma, J.H. and deBoer, W.R. 1973, Formation and regeneration of protoplasts from Pythium PRL 2142, J. Gen. Microbiol. 74: 211–217.

    Article  Google Scholar 

  • Sipiczki, M., Kucsera, J., Ulaszewski, S. and Zsolt, J. 1982, Hybridization studies by crossing and protoplast fusion within the genus Schizosaccharomyces Linder, J.Gen. Microbiol. 128: 1989–2000.

    CAS  Google Scholar 

  • Sivan, A. and Harman, G.E. 1991, Improved rhizosphere competence in a protoplast fusion progeny of Trichoderma hazianum, J. Gen. Microbiol. 137: 23–29.

    Article  Google Scholar 

  • Skata, J., Luty, J. and Kotylak, Z. 1988, Interspecific protoplast fusion between the yeasts Saccharomyces cerevisiae and Saccharomyces fermentati, Curr. Genet 13: 101–104.

    Article  CAS  Google Scholar 

  • Solis, S., Flores, M.E. and Huitron, C. 1997, Improvement of pectinase production by interspecific hybrids of Aspergillus strains, Lett. Appl. Microbiol. 24: 77–81.

    Article  CAS  Google Scholar 

  • Stasz, T.E., Harman, G.E. and Gullino, M.L. 1989, Limited vegetative compatibility following intra-and interspecific protoplast fusion in Trichoderma, Exp. Mycol. 13:364–371.

    Article  Google Scholar 

  • Stasz, T.E, Harman, G.E. and Weeden, N.F. 1988, Protoplast preparation and fusion in two biocontrol strains of Trichoderma harzianum, Mycologia 80: 141–150.

    Article  Google Scholar 

  • Suarez, T., Orejas, M. and Eslava, A.P. 1985, Isolation, regeneration and fusion of Phycomyces blakesleeanus spheroplasts, Exp. Mycol. 9: 203–211.

    Article  Google Scholar 

  • Svoboda, A. and Piedra, D. 1983, Reversion of yeast protoplasts in media containing polyethylene glycol, J. Gen. Microbiol. 129: 3371–3377.

    CAS  Google Scholar 

  • Tamaki, H. 1986, Genetic analysis of intergeneric hybrids obtained by protoplast fusion in yeasts, Curr. Genet 10: 491–494.

    Article  PubMed  CAS  Google Scholar 

  • TeBeest, D.O. and Weidemann, G.J. 1990, Preparation and regeneration of protoplasts of Colletotrichum gloeosporioides f.sp. aeschynomene, Mycologia 82: 249–255.

    Article  Google Scholar 

  • Teraoka, T., Shimura, Y., Hosokawa, D. and Watanabe, M. 1992, Giant protoplasts of Pyricularia oryzae Cavara, Ann. Phytopathol. Soc. Jpn. 58: 726–733.

    Article  CAS  Google Scholar 

  • Thrane, C., Lubeck, M., Green, H., Degefu, Y., Allerup, S., Thrane, U. and Jensen, D.F. 1995, A tool for monitoring Trichoderma harzianum: I. Transformation with the GUS gene by protoplast technology, Phytopathology 85: 1428–1435.

    Article  CAS  Google Scholar 

  • Toyama, H., Yamaguchi, K., Shinmyo, A. and Okada, H. 1984a,Protoplast fusion of Trichoderma reesei, using immature conidia, Appl. Environ. Microbiol. 47: 363–368.

    PubMed  CAS  Google Scholar 

  • Toyama, H. Yokoyama, T., Shinmyo, A. and Okada, H. 1984b,Interspecific protoplast fusion of Trichoderma, J. Biotech. 1: 25–35.

    Article  Google Scholar 

  • Tsoneva, I., Doinov, P. and Dimitrov, D.S. 1989, Electrofusion of fragile mutants of Saccharomyces cerevisiae, FEMS Microbiol. Lett. 60: 61–66.

    Google Scholar 

  • Typas, M.A. 1983, Heterokaryon incompatibility and interspecific hybridization between Verticillium alboatrum and Verticillium dahliae following protoplast fusion and microinjection, J. Gen. Microbiol. 129: 3043–3056.

    Google Scholar 

  • Ushijima, S. and Nakadai, T. 1987, Breeding by protoplast fusion of koji mold, Aspergillus sojae, Agric. Biol. Chem. 51: 1051–1057.

    Article  CAS  Google Scholar 

  • Ushijima, S., Nakadai, T. and Uchida, K. 1990, Further evidense on the interspecific protoplast fusion between Aspergillus oryzae and Aspergillus sojae and subsequent haploidization, with special reference to their production of some hydrolyzing enzymes. Agric. Biol. Chem. 54: 2393–2399.

    Article  CAS  Google Scholar 

  • Ushijma, S. Nakadai, T. and Uchida, K., 1991, Interspecific electrofusion of protoplasts between Aspergillus oryzae and Aspergillus sojae, Agric. Biol. Chem 55: 129–136.

    Article  Google Scholar 

  • Vagvolgyi, Cs. and Ferenczy, L. 1991, Isolation of nuclei from Aspergillus nidulans protoplasts, FEMS Microbiol. Lett. 82: 247–252.

    Article  Google Scholar 

  • Vagvolgyi, Cs., Bruckner, B. and Franko, A. 1990, Size dependent regeneration of Gibberella protoplasts, Acta Microbiol. Hungarica 37: 375–378.

    CAS  Google Scholar 

  • Vainstein, M.H. and Peberdy, J.F. 1991, Location of invertase in Aspergillus nidulans: release during hyphal wall digestion and secretion by protoplasts, Mycol. Res. 95: 1270–1274.

    Article  CAS  Google Scholar 

  • Vidoli, R., Yamazak, H., Nasim, A. and Veliky, I.A. 1982, A novel procedure for the recovery of hybrid products from protoplast fusion, Biotechnol. Lett. 12: 781–784.

    Article  Google Scholar 

  • Villanueva, J.R. and Garcia Acha, I. 1971, Production and use of fungal protoplasts, in: Methods in Microbiology, Vol. IV, C. Booth, ed., Academic Press, New York.

    Google Scholar 

  • Wagner, R.E. and Wilkinson, H.T. 1993, Spheroplast and protoplast formation from cysts of Phytophthora sojae treated with digestive enzymes, Mycologia. 85: 131–136.

    Article  Google Scholar 

  • Wilson, J.J., Khachatourians, G.G. and Ingledew, W.M. 1982, Protoplast fusion in the yeast Schwanniomyces alluvius, Mole.Gen. Genet. 186: 95–100.

    Article  CAS  Google Scholar 

  • Witkowska, D., Stempniewicz, R. and Wojatowicz, M. 1997, Production of lytic enzymes using Trichoderma reesei M7-1 in yeast protoplast formation, Pol. J. Food and Nutrition Sci. 6: 51–60.

    CAS  Google Scholar 

  • Wostemeyer, J. and Brockhausen-Rohdemann, E. 1987, Inter-mating type protoplast fusion in the zygomycete Absidia glauca, Curr. Genet 12: 435–441.

    Article  Google Scholar 

  • Yamashita, K., Fukuda, H., Murata, K. and Kimura, A. 1981, Transfer of mitochondria of Hansenula wingei into protoplasts of Saccharomyces cerevisiae by mini-protoplast fusion, FEBS Lett. 132:305–307.

    Article  Google Scholar 

  • Yanagi, S.O., Moruna, M., Kawasumi, T., Hino, A., Kito, M. and Takebe, I. 1985, Conditions for isolation and colony formation by mycelial protoplasts of Coprinus macrorhizus, Agric. Biol. Chem. 49: 171–179.

    Article  CAS  Google Scholar 

  • Yanagida, T., Okamura, C., Itoh, K., Oki, T. and Tachibana, S. 1996, A trial for formation of a fusant by protoplast fusion of Gibberella fujikuroi strains, Bull. Ehime Univ. Forest C (34): 93–106.

    Google Scholar 

  • Yang, H.A., Tommerup, I.C., Sivasithamparam, K. and O’Brien, P.A. 1992, Heterokaryon formation with homokaryons derived from protoplasts of Rhizoctonia solani anastomosis group eight, Exp. Mycol. 16: 268–278.

    Article  Google Scholar 

  • Yang, H.A., Zhou, J., Sivasithamparam, K. and O’Brien, P.A. 1994, Variation in culture morphology and pathogenicity among protoplast-regenerated strains of Rhizoctonia solani, FEMS Microbiol. Lett. 115: 83–86.

    Article  Google Scholar 

  • Zhao, J. and Chang, S.T. 1995, Intraspecific hybridization between Coprinus cinereus and Schizophyllum commune by PEG-induced protoplast fusion and electrofusion, World J. Microbiol. Biotech. 11: 585–590.

    Article  CAS  Google Scholar 

  • Zhao, J. and Chang, S.T. 1996, Intergeneric hybridization between Pleurotos ostreatus and Schizophyllum commune by PEG induced protoplast fusion, World J. Microbiol. Biotech. 12: 573–578.

    Article  Google Scholar 

  • Znidarsic, P., Pavko, A. and Komel, R. 1992, Laboratory — scale biosynthesis of Trichoderma mycolytic enzymes for protoplast release from Cochliobolus lunatus, J. Ind. Microbiol. 9: 115–119.

    Article  CAS  Google Scholar 

  • Zvereva, E.A., Obraztsova, I.N., DeMina, V.A. and Nutsubidge, N.N. 1996, Effect of protoplast formation and cell regeneration on the biosynthesis capability of the mycelial fungus Aspergillus parasiticus, Biochem. (Moscow) 60: 1383–1386.

    Google Scholar 

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Sumeet, Mukerji, K.G. (1999). Protoplast Fusion in Disease Control. In: Mukerji, K.G., Chamola, B.P., Upadhyay, R.K. (eds) Biotechnological Approaches in Biocontrol of Plant Pathogens. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4745-7_9

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