Rat Carbonyl Reductase
Carbonyl reductase (secondary alcohol:NADP+ oxidoreductase, EC 1. 1. 1. 184) catalyzes the NADPH-dependent reduction of a variety of endogenous and xenobiotic carbonyl compounds (Wermuth, 1981; Jarabak et al., 1983; Nakayama et al., 1985). Structurally it belongs to the short-chain dehydrogenase/reductase (SDR) family which includes steroid, prostaglandin, pterin and retinol metabolizing enzymes (Jörnvall et al. 1995); however the physiological substrate(s) of carbonyl reductase is (are) not known. In man, the enzyme is expressed in essentially all tissues with highest activity in liver, kidney and brain (Wirth and Wermuth, 1992). Evidence from sequence analysis of cDNAs (Wermuth et al., 1988; Forrest et al., 1990) as well as genomic DNA (Forrest et al., 1991) suggests the presence of a single gene which consists of three exons and two introns and spans about 3 kb on chromosome 21q22. 1. The 5′-untranslated region contains a GC-rich island but no TATA or CAAT consensus sequences (Forrest et al., 1991) consistent with the constitutive expression of the enzyme in essentially all tissues.
KeywordsTranslation Start Site High Protein Concentration Pregnant Mare Serum Gonadotropin Carbonyl Reductase Guanidinium Thiocyanate
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- Jörnvall, H., Persson, B., Krook, M., Atrian, S., Gonzàlez-Duarte, R., Jeffery, J. and Ghosh, D. (1995) Short-chain dehydrogenases/reductases (SDR), Biochemistry 34, 6004–6013.Google Scholar