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The Roles of Acetaldehyde Dehydrogenases in Saccharomyces Cerevisiae

  • Wayne Tessier
  • Mark Dickinson
  • Melvin Midgley
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 463)

Abstract

This work was undertaken to try to understand the routes that are used for synthesis of acetyl-CoA in Saccharomyces cerevisaeunder a variety of nutritional conditions. It is a topic of interest in connection with the production of flavour compounds in beer and lager. Distinct physiological roles have been proposed for the Mg2+ activated, NADP+-dependent acetaldehyde dehydrogenase (Mg-ACDH) and the K+-activated NAD+/NADP+ dependent mitochrial enzyme (K-ACDH) (Llorente&de Castro 1977). The former enzyme was thought to function in acetate formation from acetaldehyde derived from pyruvate through the action of pyruvate decarboxylase. The latter enzyme has been implicated in acetate formation from acetaldehyde derived from ethanol. On this basis, disruption mutants for each of the enzymes would be expected to have markedly different potential when grown on 5% glucose, where mitochondrial function is severely repressed and on 1 % ethanol where mitochondrial function is developed.

Keywords

Saccharomyces Cerevisiae Specific Growth Rate Pyruvate Decarboxylase Acetate Formation Disruption Mutant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Wayne Tessier
    • 1
  • Mark Dickinson
    • 1
  • Melvin Midgley
    • 1
  1. 1.Department of Biological SciencesUniversity of HullUK

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