The Roles of Acetaldehyde Dehydrogenases in Saccharomyces Cerevisiae
This work was undertaken to try to understand the routes that are used for synthesis of acetyl-CoA in Saccharomyces cerevisaeunder a variety of nutritional conditions. It is a topic of interest in connection with the production of flavour compounds in beer and lager. Distinct physiological roles have been proposed for the Mg2+ activated, NADP+-dependent acetaldehyde dehydrogenase (Mg-ACDH) and the K+-activated NAD+/NADP+ dependent mitochrial enzyme (K-ACDH) (Llorente&de Castro 1977). The former enzyme was thought to function in acetate formation from acetaldehyde derived from pyruvate through the action of pyruvate decarboxylase. The latter enzyme has been implicated in acetate formation from acetaldehyde derived from ethanol. On this basis, disruption mutants for each of the enzymes would be expected to have markedly different potential when grown on 5% glucose, where mitochondrial function is severely repressed and on 1 % ethanol where mitochondrial function is developed.
KeywordsSaccharomyces Cerevisiae Specific Growth Rate Pyruvate Decarboxylase Acetate Formation Disruption Mutant
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