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Susceptibility of Hepatoma Cells to Lipid Peroxidation and Adaptation of ALDH 3C Activity to Iron-Induced Oxidative Stress

  • Margherita Ferro
  • Anna Maria Bassi
  • Susanna Penco
  • Giuliana Muzio
  • Rosa A. Canuto
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 463)

Abstract

Free rcal iron-induced oxidative stress causes membrane lipid peroxidation and conseqt cell damage (Bacon et al., 1985), a process that, in rat liver, is significantly lower tumor than normal tissue (Dianzani et al., 1984, 1986; Borrello et al., 1985). Factors contriing to this difference include a reduction in cytochrome P450 (an enzyme systemplicated in the production of initiating and propagating radicals), a reduction in the peidizable substrate arachidonic acid (ARA), an increase in the lipid soluble anti- oxidan-tocopherol (Borrello et al., 1985; Cheeseman et al., 1988).

Keywords

Lipid Peroxidation Arachidonic Acid Hepatoma Cell Hepatoma Cell Line Tukey Kramer Test 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Bacon B.R., Tavill A.S., Brittenham G.M., Parck C.H., and Recknagel R.O., 1985, in:“Free Radicals in Liver Injury” (Poli G., Cheeseman K.H., Dianzani M.U., Slater T.F., eds.), pp. 49–57, IRL Press, Oxford.Google Scholar
  2. Bast A. and Haenen G.R.M.M., 1984, Cytochrome P450 and glutathione: what is the significance of their interrelationship in lipid peroxidation?, Trends Biochem. Sci., 9, 510–513.CrossRefGoogle Scholar
  3. Borenfreund E. and Puerner J.A., 1985, Toxicity determined in vitro by morphological alterations and neutral red absorbtion, Toxicol. Letts., 24: 119–124.CrossRefGoogle Scholar
  4. Borrello S., Minotti G., Palombini G., Grattagliano A., and Galeotti T., 1985, Superoxide-dependent lipid peroxidation and vitamine E content of microsomes from hepatomas with different growth rates, Arch. Biochem. Biophys., 238: 588–595.PubMedCrossRefGoogle Scholar
  5. Bradford M.M., 1976, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72: 248–254.PubMedCrossRefGoogle Scholar
  6. Cantoni O., Sestili P., Brandi G., and Cattabeni F., 1994, The L-histidine-mediated enhancement of hydrogen peroxide-induced cytotoxicity is a general response in cultured mammalian cell lines and is always associated with the formation of DNA double strand breaks. FEBS Letts., 353: 75–78.CrossRefGoogle Scholar
  7. Canuto R.A., Garcea R., Biocca M.E., Pascale R., Pirisi L., and Feo F., 1983, The subcellular distribution and properties of aldhyde dehydrogenase of hepatoma AH-130, Eur. J., Cancer. Clin. Oncol., 19: 389–400.CrossRefGoogle Scholar
  8. Canuto R.A., Muzio G., Biocca M.E., and Dianzani M.U., 1991, Lipid peroxidation in rat AH-130 hepatoma cells enriched in vitro with arachidonic acid, Cancer Res., 51: 4603–4608.PubMedGoogle Scholar
  9. Canuto R.A., Muzio G., Bassi A.M., Maggiora M., Leonarduzzi G., Lindahl R., Dianzani M.U., and Ferro M., 1995, Enrichment with arachidonic acid increases the sensitivity of hepatoma cells to the cytotoxic effects of oxidative stress, Free Rad. Biol. Med., 18: 287–293.PubMedCrossRefGoogle Scholar
  10. Canuto R.A., Ferro M., Maggiora M., Fédéra R., Brossa O., Bassi A.M., Lindahl R., and Muzio G., 1996, In hepatoma cell lines restored lipid peroxidation affects cell viability inversely to aldehyde metabolizing enzyme activity. In:“Enzymology and Molecular Biology of Carbonyl Metabolism 6”, (Weiner et al. eds), pp. 113–122, Plenum Press, New York.Google Scholar
  11. Cheeseman K.H., Emery S., Maddix S.P., Slater T.F., Burton G.W., and Ingold K.U., 1988, Studies on lipid peroxidation in normal and tumor tissues, Biochem. J., 250: 247–252.PubMedGoogle Scholar
  12. Dianzani M.U., Canuto R.A., Rossi M.A., Poli G., Garcea R., Biocca M.E., Cecchini G., Ferro M., and Bassi A.M., 1984, Further experiments on lipid peroxidation in transplanted and experimental hepatomas, Toxicol. Pathol. 12: 189–199.PubMedCrossRefGoogle Scholar
  13. Dianzani M.U., Poli G., Canuto R.A., Rossi M.E., Biasi F., Cecchini G., Muzio G., Ferro M., and Esterbauer H., 1986, New data on kinetics of lipid peroxidation in experimental hepatomas and preneoplastic nodules, Toxicol. Pathol.,14: 404–410.PubMedCrossRefGoogle Scholar
  14. Donato T., Bassi A.M., Gömez-Lechön M.J., Penco S., Herrero E., Adamo D., Castell J.V., and Ferro M., 1994, Evaluation of the xenobiotic biotransformation capability of six rodent hepatoma cell lines in comparison with rat hepatocytes. In vitro Cell Dev. Biol. 30A: 574–580.CrossRefGoogle Scholar
  15. Esterbauer H., Zollner H., and Schaur R.J., 1990, Aldehydes formed by lipid peroxidation:mechanisms of formation, occurence and determination, in:“Membrane Lipid Oxidation”, Vol.1, (Vigo Pelfrey C, ed.) Boca Raton. FL.Google Scholar
  16. Hammer A., Ferro M., Tillian H.M:, Tatzber F., Zollner H., Schauenstein E., and Schaur R.J., 1997, Effects of oxi-dative stress by iron on 4-hydroxynonenal formation and proliferative activity in hepatomas of different degrees of differentiation, Free Rad. Biol. Med., 23: 26–33.PubMedCrossRefGoogle Scholar
  17. Iguchi H., Kojo S., and Ikeda M, 1993, Lipid peroxidation and disintegration of the cell membrane structure in cultures of of rat lung fibroblasts treated with asbestos, J. Appl. Toxicol, 13: 269–275.PubMedCrossRefGoogle Scholar
  18. Koster J.F., and Slee R.G., 1980, Lipid peroxidation of rat liver microsomes, Biochem., Biophys. Ada, 620: 489–499.CrossRefGoogle Scholar
  19. Lambert N., Chambers S.J., Plumb G.W., and Williamson G., 1996, Human cytochrome P450 are pro-oxidants in iron/ascorbate-initiated microsomal lipid peroxidation, Free Rad. Res., 24: 177–185.CrossRefGoogle Scholar
  20. Lindahl R., 1992, Aldehyde dehydrogenases and their role in carcinogenesis, Critical Rev. Biochem. Mol. Biol, 27: 283–335.CrossRefGoogle Scholar
  21. Mosmann T., 1983, Rapid colorimetric assay for cellular growth and survival: application to proliferation and cy-totoxicity assays, J. Immunol. Meth., 65: 55–63.CrossRefGoogle Scholar
  22. Ohmori S., Misaizu T., Nakamura T., Takano N., Kitagawa H., and Kitada M., 1993, Differential role of lipid peroxidation between rat P450 1A1 and P450 1A2, Biochem. Pharmacol, 46: 55–60.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Margherita Ferro
    • 1
  • Anna Maria Bassi
    • 2
  • Susanna Penco
    • 1
  • Giuliana Muzio
    • 2
  • Rosa A. Canuto
    • 2
  1. 1.Department of Experimental MedicineUniversity of GenoaGenovaItaly
  2. 2.Department of Clinical and Biological Sciences St. Louis Gonzaga HospitalUniversity of TurinOrbassanoItaly

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