Susceptibility of Hepatoma Cells to Lipid Peroxidation and Adaptation of ALDH 3C Activity to Iron-Induced Oxidative Stress
Free rcal iron-induced oxidative stress causes membrane lipid peroxidation and conseqt cell damage (Bacon et al., 1985), a process that, in rat liver, is significantly lower tumor than normal tissue (Dianzani et al., 1984, 1986; Borrello et al., 1985). Factors contriing to this difference include a reduction in cytochrome P450 (an enzyme systemplicated in the production of initiating and propagating radicals), a reduction in the peidizable substrate arachidonic acid (ARA), an increase in the lipid soluble anti- oxidan-tocopherol (Borrello et al., 1985; Cheeseman et al., 1988).
KeywordsLipid Peroxidation Arachidonic Acid Hepatoma Cell Hepatoma Cell Line Tukey Kramer Test
Unable to display preview. Download preview PDF.
- Bacon B.R., Tavill A.S., Brittenham G.M., Parck C.H., and Recknagel R.O., 1985, in:“Free Radicals in Liver Injury” (Poli G., Cheeseman K.H., Dianzani M.U., Slater T.F., eds.), pp. 49–57, IRL Press, Oxford.Google Scholar
- Cantoni O., Sestili P., Brandi G., and Cattabeni F., 1994, The L-histidine-mediated enhancement of hydrogen peroxide-induced cytotoxicity is a general response in cultured mammalian cell lines and is always associated with the formation of DNA double strand breaks. FEBS Letts., 353: 75–78.CrossRefGoogle Scholar
- Canuto R.A., Ferro M., Maggiora M., Fédéra R., Brossa O., Bassi A.M., Lindahl R., and Muzio G., 1996, In hepatoma cell lines restored lipid peroxidation affects cell viability inversely to aldehyde metabolizing enzyme activity. In:“Enzymology and Molecular Biology of Carbonyl Metabolism 6”, (Weiner et al. eds), pp. 113–122, Plenum Press, New York.Google Scholar
- Esterbauer H., Zollner H., and Schaur R.J., 1990, Aldehydes formed by lipid peroxidation:mechanisms of formation, occurence and determination, in:“Membrane Lipid Oxidation”, Vol.1, (Vigo Pelfrey C, ed.) Boca Raton. FL.Google Scholar
- Hammer A., Ferro M., Tillian H.M:, Tatzber F., Zollner H., Schauenstein E., and Schaur R.J., 1997, Effects of oxi-dative stress by iron on 4-hydroxynonenal formation and proliferative activity in hepatomas of different degrees of differentiation, Free Rad. Biol. Med., 23: 26–33.PubMedCrossRefGoogle Scholar