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Oxidation of Fish Sarcoplasmic Reticular Lipids and Proteins

  • Ayla Soyer
  • Herbert O. Hultin

Abstract

The kinetics of oxidation of lipids and proteins of cod sarcoplasmic reticulum in system in which ferric iron(10µM)is reduced to the active ferrous form either nonenzymically with ascorbate,or enzymically with NADH was studied.Lipid and protein oxidations occurred at different rates of formation of thiobarbituric acid reactive substances(TBARS)was less rapid,but the ultimate amount of TBARS produced was greater than in the nonenzymic system.Loss of ultimate amount of TBARS produced was greater than in the nonenzymic system.Loss of protein sulfhydryl groups,presumably by oxidation to disufides,occurred to a great extent in both system.Oxidations of sulfhydryl groups was accompanied by polymerization of the major membrane protein,Ca2+-ATPase,as determined by SDS-PAGE

Keywords

Sarcoplasmic Reticulum Lipid Oxidation Ferric Iron Protein Oxidation Fish Muscle 
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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Ayla Soyer
    • 1
  • Herbert O. Hultin
    • 2
  1. 1.Food Engineering Department, Faculty of AgricultureAnkara UniversityDiskapiTurkey
  2. 2.Department of Food ScienceUniversity of Massachusetts/AmherstGloucesterUSA

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