Production of Recombinant Blood Factors in Transgenic Plants

  • Manfred Theisen
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 464)

Abstract

The production of recombinant proteins for therapeutic use in transgenic plants has become an alternative to the isolation of these molecules from natural or other recombinant sources. Plant bioreactors offer the possibility of an inexpensive large scale production of high volumes of recombinant proteins with increased safety concerning contaminations with human pathogens. Several laboratories are involved in the commercial production of the first recombinant products, such as antibodies or recombinant human hemoglobin, from transgenic plants.

Keywords

Biomass Fermentation Maize Hepatitis Albumin 

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References

  1. Dalsgaard, K., Uttenthal, A., Jones, T.D., Zu, F., Merryweather, A., Hamilton, W.D.O., Langeveld, J.P.M., Boshuizen, R.S., Kamstrup, S., Lomonossoff, G.P., Porta, C., Vela, C., Casal, J.I., Meloen, R.H. and Rodg-ers, P.B. Plant-derived vaccine protects target animals against a viral disease. Nature Biotechnology 1997, 15,248–252.PubMedCrossRefGoogle Scholar
  2. Dieryck, W., Pagnier, J., Poyart, C., Marden, M.C., Gruber, V., Bournat, P., Baudino, S. and Merot, B. Human haemoglobin from transgenic tobacco. Nature (London) 1997, 386, 29–30.CrossRefGoogle Scholar
  3. Dodet, B. Production de protéines recombinantes: quel system choisir? Biofutur 1990, 91, 20–29.Google Scholar
  4. Gomord, V., Sailer J-P. et Faye L. Plante et médicament, un nouveau départ. Biofutur 1996, 154, 26–30.CrossRefGoogle Scholar
  5. Gomord, V. and Faye, L. Signals and mechanisms involved in intracellular transport of secreting proteins in plants. Plant Physiol. Biochem. 1996, 34, 165–181.Google Scholar
  6. Haq, T.A., Mason, H.S., Clements, J.C. and Arntzen, C.J. Oral immunization with a recombinant bacterial antigen produced in transgenic plants. Science 1995 268, 714–716.PubMedCrossRefGoogle Scholar
  7. Hennighausen, L., Ruiz, L. and Wall, R. Transgenic animals — production of foreign proteins in milk. Curr. Opin. Biotechnol. 1990, 1, 74–78.PubMedCrossRefGoogle Scholar
  8. Ma, J.K.C., Hiatt, A., Hein, M., Vine, N.D., Wang, F., Stabila, P., Van Dolleweerd, C., Mostov, K. and Lehner, T. Generation and assembly of secretory antibodies in plants. Science 1995, 268, 116–719.CrossRefGoogle Scholar
  9. Mason, H.S., Ball, J.M., Shi, J.J., Jiang, X., Estes, M.K. and Arntzen, C.J. Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice. Proc. Natl Acad. Sci. USA 1996, 93, 5335–5340.PubMedCrossRefGoogle Scholar
  10. McGravey, P.B., Hammond, J., Dienelt, M.M., Hooper, D.C., Fu, Z.F., Dietzschold, B., Kprowski, H. and Michaels, F.H. Expression of the rabies virus glycoprotein in transgenic tomatoes. Bio/Technology 1995, 13, 1484–1487.CrossRefGoogle Scholar
  11. McLain L., Durrani, Z., Wisniewski, L.A., Porta, C., Lomonossof, G.P. and Dimmock, N.J. Stimulation of neutralizing antibodies to human immunodeficiency virus type 1 in three strains of mice immunized with a 22 amino acid peptide of gp 41 expressed on the surface of a plant virus. Vaccine 1996 14, 799–809.PubMedCrossRefGoogle Scholar
  12. Moffat, A.S. Exploring transgenic plants as a new vaccine source. Science 1995, 268, 658–660.PubMedCrossRefGoogle Scholar
  13. Porta, G., Spall, V, Loveland, J., Johnson, J., Barker, P. and Lomonossoff, G. Development of cowpea mosaic virus as a high-yield system for the presentation of foreign peptides. Virology 1994, 202, 949–955.PubMedCrossRefGoogle Scholar
  14. Sijmons, P.C., Dekker, B.M.M., Schrammeijer, B., Verwoerd, T.C., Van den Elzen, P.J.M. and Hoekema, A. Production of correctly processed human serum albumin in transgenic plants. Bio/Technology 1990, 8, 217–221.PubMedCrossRefGoogle Scholar
  15. Thanavala, Y., Yang, Y.F., Lyons, P., Mason, H.S. and Arntzen C. Immunogenicity of transgenic plant-derived hepatitis B surface antigen. Proc. Nati Acad. Sei. USA 1995, 92, 3358–3361.CrossRefGoogle Scholar
  16. Verbert, A. La glycosylation des protéines recombinantes. Biofutur 1993, 125, 40–44.Google Scholar
  17. Wandelt, C.I, Rafiqul M., Khan, L, Craig, S., Schroeder, H.E., Spencer D. and Higgins, T.J.V. Vicilin with car-boxy-terminal KDEL is retained in the endoplasmic reticulum to high levels in leaves of transgenic plants. Plant J. 1992, 181–192.Google Scholar
  18. Whitelam, G.C. and Cockburn, W. Antibody expression in transgenic plants.Trends Plant Sci. 1996, 8, 268–272.Google Scholar

Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Manfred Theisen
    • 1
  1. 1.Meristem TherapeuticsClermont-FerrandFrance

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