Laser-Assisted Optical Rotational Red Cell Analyzer (LORCA) in Clinical Practice

Hemorheological Kinetics and Tissue Oxygenation
  • G. Cicco
  • A. J. van der Kleij
  • G. D. Stingi
  • M. S. Tarallo
  • A. Pirrelli
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 471)


Relevant hemodynamic changes in arterial hypertension (i.e. vasoconstriction induced by an organic arterial wall thickening or a functionally induced vasospasm) can lead to increased peripheral resistance. In the microvasculature of hypertensive subjects it is often possible to detect decreased peripheral perfusion together with hemorheological alterations (such as the presence of red blood cell aggregates, decreased erythrocyte deformability). These hemorheological changes can raise blood pressure (increasing the resistance of the blood flow in the microvasculature) and may finally induce a reduction in peripheral tissue oxygenation. These conditions are negatively influenced by lipoido-proteinosis, diabetes and smoking, which very important risk factors in hypertensives for acute myocardial infarction, transient ischemic attack, ictus, and peripheral occlusive arterial disease (Cesarone, 1992; Cicco, 1993, 1998). It has been shown that a good pharmacological control of the blood pressure and the lipoidoproteinosis influence the hemorheological patterns in the microvasculature, improving peripheral perfusion and tissue oxygenation (Cicco, 1994, 1995, 1997).


Tissue Oxygenation Peripheral Occlusive Arterial Disease Perfusion Index Mean Blood Pressure Peripheral Perfusion 
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  1. Bessis M (1978) Reintegrazione degli studi di sangue. Piccin ed. Padova.Google Scholar
  2. Cesarone MR, Laurora G, and Belcaro GU (1992) Microcirculation in systemic hypertension. Angiology; 43(11):899–903.PubMedCrossRefGoogle Scholar
  3. Cicco G and Pirrelli A (1993) Emoreologia clinica ed ipertensione arteriosa. Min. Cardioangiol.; 41:6:213–223.Google Scholar
  4. Cicco G, Dolce E, Mancini M, and Pirrelli A (1994) Arteriosclerosis and hemorheology in hypertension: organ damage. Med. J. Surg. Med.; 3:131–138.Google Scholar
  5. Cicco G, Dolce E, Vicenti P, Gigante G, and Pirrelli A (1995) Peripheral oxygen release evaluation using 3 transcutaneous oximeters contemporarily on patients with hypertension and lipoidoproteinosis. Clin. Hemorheology; 3(15):P25 539.Google Scholar
  6. Cicco G, Vicenti P, Pirrelli A, and van der Kleij AJ (1997) Peripheral perfusion and tissue oxygenation improvement induced by antihypertensive medication combined with lipoidoproteinosis treatment. In: Adv. in Exp. Med. Biol. 411:261–266.CrossRefGoogle Scholar
  7. Cicco G and Pirrelli A (1998) Hemorheology and tissue oxygenation transport during hypertension. Clin. Hemorheology and Microcirculation IOS Press, Amsterdam; 19; 1:61–63.Google Scholar
  8. Cicco G (1998) Red blood cell deformability; blood viscosity and tissue oxygenation in hypertension. 2nd Int. Conf. Hemorheology and Tissue Oxygenation in Hypertension Abstract Book L3, 27-28.Google Scholar
  9. Forconi S, Guerrini M, Cappelli R, Sarri P, and Furesi L (1989) Rapporti fra endotelio e cellule circolanti a livello microcircolatorio influenza su coagulazione, fibrinolisi, mobilità della parete vascolare ed emoreologia. In: Tesi M. Microcircolazione 1989, Monduzzi Editore Bologna 145-151.Google Scholar
  10. Forconi S, Turchetti V, De Matteis C, Leoncini F, Pierolini F, Tribalzini L, Pastorino G, and Altavilla R (1994) Behaviour of blood viscosity, intra-erythrocytic calcium, total erythrocyte morphology in ischaemic diseases. Proceed. XVII Eur. Conf. Microcicul. Roma 68.Google Scholar
  11. Forconi S (1997) Relationship between erythrocyte morphology and deformability—Boletim de Societade Portuguesa de Emorreologia e Microcirculaçao, X Eur. Conf. on Clin. Hemorheology L2.Google Scholar
  12. Forconi S (1998) Morphological approach to red cell deformability in a clinical environment. 2nd Int. Conf. Hemorheology and Tissue Oxygenation in Hypertension Abstract Book L28, 79.Google Scholar
  13. Fredrickson DS, Goldstein JL, and Brown JS (1978) The Familial Hyperlipoproteinemias. In: The metabolic basis of inherited disease. Stanbury JB; Wyngaarden JB, and Fredrickson DS eds. New York, McGraw Hill 604–655.Google Scholar
  14. Hardeman MR, Goedhart PT, Dobbe JGG, and Lettinga KR (1994) Laser-Assisted Optical Rotational Red Cell Analyzer (LORCA) I: a new instrument for measurement of various structural hemorheological parameters. Clin. Hemorheology 14:605–618.Google Scholar
  15. Hardeman MR, Goedhart PT, and Schut NH (1994) Laser. Assisted Optical Rotational Red Cell analyzer (LORCA) II: red blood cell deformability; elongation index versus cell transit analyzer. Clin. Hemorheology 14:619–630.Google Scholar
  16. Hardeman MR and Goedhart PT (1994) Laser-Assisted Optical Rotational Red Cell Analyzer (LORCA): Red Cell Aggregometry. In: J.F. Stoltz ed., Editions Médicales Internationales Paris.Google Scholar
  17. Hardeman MR and Goedhart PT (1995) Laser Assisted Optical Rotational Red Cell Analyzer (LORCA): “viscoscan” of RBC deformability and estimation of RBC internal viscosity. Biorheology, 32:357.CrossRefGoogle Scholar
  18. Hardeman MR (1998) Clinical hemorheology placed in to hypertension: introduction and implementation of new methodology. Clin. Hemorheology and Microcirculation, IOS Press, Amsterdam 19; 1:50–54.Google Scholar
  19. Hardeman MR (1998) LORCA, a new versatile hemorheological instrument. 2nd Int. Conf. Hemorheology and tissue oxygenation in Hypertension Abstract Book C27, 77, 78.Google Scholar
  20. Hauser CJ and Shoemaker W (1983) Use of a transcutaneous pO2 Regional Perfusion Index to quantify tissue perfusion in peripheral vascular disease. Am. J. Surg. 197:337–343.Google Scholar
  21. Kleij van der AJ and Bakker DJ (1996) Oximetry. In: “Handbook of hyperbaric medicine” G Oriani, A Marroni, and F Wattel (Ed. Springer-Verlag Italy Milano) 670–675.Google Scholar
  22. Kleij van der AJ (1998) Which method can be used to evaluate the tissue oxygenation? Clin. Hemorheology and Microcirculation, IOS Press, Amsterdam 19; 1:55–60.Google Scholar
  23. Kleij van der AJ (1998) Invasive and non-invasive pO2 measurements in Clinical Practice—2nd Int. Conf. Hemorheology and tissue oxygenation in hypertension. Abstract Book C23, 69-70.Google Scholar
  24. Zipursky A, Brown E, Palko J, and Brown E (1983) The erythrocyte differential count in newborn infants. Am. J. of Ped. Haemat. Oncol. 45-51.Google Scholar

Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • G. Cicco
    • 1
  • A. J. van der Kleij
    • 2
  • G. D. Stingi
    • 1
  • M. S. Tarallo
    • 1
  • A. Pirrelli
    • 1
  1. 1.Department of Clinical Methodology and Medico-Surgical Technologies Internal Medicine and Hypertension Lab. of Hemorheology and Oxygen Transport to TissueUniversity of BariItaly
  2. 2.Department of Surgery/Hyperbaric MedicineAcademic Medical Center, University of AmsterdamNetherlands

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