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L-Tryptophan-Kynurenine Pathway Metabolite 3-Hydroxyanthranilic Acid Induces Apoptosis in Macrophage-Derived Cells Under Pathophysiological Conditions

  • Toshiko Morita
  • Kuniaki Saito
  • Masao Takemura
  • Naoya Maekawa
  • Suwako Fujigaki
  • Hidehiko Fujii
  • Hisayasu Wada
  • Shoji Takeuchi
  • Akio Noma
  • Mitsuru Seishima
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 467)

Abstract

Accumulation of L-kynurenine and 3-hydroxyanthranilic acid (3HAA) occurs in the monocyte-derived cells following immune stimulation, and may derive from L-tryptophan following induction of indoleamine-2,3-dioxygenase. In the present study, we evaluate the possibility that 3HAA acts as an endogenous inducer of monocyte/macrophage apoptosis. Supplementation with 200 μM of 3HAA, but not other L-tryptophan metabolites tested, significantly increased the number of apoptotic cells in both THP-1 and U937 cells. Catalase, superoxide dismutase and manganese ions markedly enhanced apoptosis in the presence of 3HAA in these cells. The present results suggest that 3HAA induces the macrophage/monocyte apoptosis under certain conditions, which may be relevant to patho-physiology of inflammatory conditions.

Keywords

U937 Cell Quinolinic Acid Anthranilic Acid Kynurenic Acid Picolinic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1999

Authors and Affiliations

  • Toshiko Morita
    • 1
  • Kuniaki Saito
    • 1
  • Masao Takemura
    • 1
  • Naoya Maekawa
    • 1
  • Suwako Fujigaki
    • 1
  • Hidehiko Fujii
    • 1
  • Hisayasu Wada
    • 1
  • Shoji Takeuchi
    • 1
  • Akio Noma
    • 1
  • Mitsuru Seishima
    • 1
  1. 1.Department of Laboratory MedicineGifu University School of MedicineGifuJapan

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