Abstract
The need to define the actions of paracrine and autocrine signaling factors against a background of systemic hormones led to the development of the slow-release implant technique. Elvax40P® is an ethylene vinyl acetate copolymer. Formed into a matrix containing test materials such as hormones or growth factors, Elvax releases metered amounts and can be used to treat small zones within the mammary gland. Importantly, Elvax is generally nondenaturing to proteins and steroids and does not elicit a tissue response. To date, Elvax experiments have been used to help define the in vivo roles of a variety of growth factors and hormones. Here we describe the fabrication and surgical techniques necessary to use this material.
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Abbreviations
- BSA:
-
bovine serum albumin
- DCA:
-
deoxycorticosterone acetate
- DCM:
-
dichloromethane
- EGF:
-
epidermal growth factor
- Elvax:
-
Elvax 40W®,ethylene vinyl acetate copolymer
- IGF:
-
insulin-like growth factor
- LA:
-
lobuloalveolar
- TPA:
-
tetradecanoyl phorbol myristic acid
- TGF:
-
transforming growth factor
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© 2000 Springer Science+Business Media New York
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Silberstein, G.B., Daniel, C.W. (2000). Slow-Release Plastic Pellets (Elvax) for Localized In Situ Treatments of Mouse and Rat Mammary Tissue. In: Ip, M.M., Asch, B.B. (eds) Methods in Mammary Gland Biology and Breast Cancer Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4295-7_8
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DOI: https://doi.org/10.1007/978-1-4615-4295-7_8
Publisher Name: Springer, Boston, MA
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