Adenoviral and Transgenic Approaches for the Conditional Deletion of Genes from Mammary Tissue

  • Kay-Uwe Wagner
  • Edmund B. RuckerIII
  • Lothar Hennighausen
Chapter

Abstract

Over the past decade the tools of gene targeting have permitted an unparalleled insight into genetic pathways that control mammary development and tumorigenesis in the mouse. However, the role of many genes in development and disease remains elusive, since their deletion from the mouse genome is either lethal for the mouse or does not mimic human disease progression. Thus, targeting gene deletions or modifications precisely to mammary epithelial cells during distinct time windows is a promising approach to establish high-fidelity mouse models for the study of development and disease.

Keywords

Glycerol Estrogen Lactate Recombination Syringe 

Abbreviations

Ad

adenoviral

BLG

β-lactoglobulin

CMV

cytomegalovirus

ES

embryonic stem

GOI

gene of interest

LTR

long terminal repeat

MMTV

mouse mammary tumor virus

WAP

whey acidic protein

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Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Kay-Uwe Wagner
    • 1
  • Edmund B. RuckerIII
    • 1
  • Lothar Hennighausen
    • 1
  1. 1.Laboratory of Genetics and Physiology, National Institute of Diabetes, Digestive, and Kidney DiseasesNational Institutes of HealthBethesdaUSA

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