Abstract
The intent of this chapter is to briefly present a successful protocol for establishing mouse mammary epithelial cell lines in a reproducible manner. The chapter describes the establishment and characterization of cell lines in vitro, the requirements for in vivo transplantability, and tissue and cell banking. Unique reagents are also described. This method is applicable to normal as well as transformed mammary cell populations and could readily be exploited to establish mammary cell lines from transgenic and knockout mice as well as conventional mice.
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Abbreviations
- (ECM):
-
extracellular matrix
- (ABS):
-
adult bovine serum
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© 2000 Springer Science+Business Media New York
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Medina, D., Kittrell, F. (2000). Establishment of Mouse Mammary Cell Lines. In: Ip, M.M., Asch, B.B. (eds) Methods in Mammary Gland Biology and Breast Cancer Research. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4295-7_13
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DOI: https://doi.org/10.1007/978-1-4615-4295-7_13
Publisher Name: Springer, Boston, MA
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