Abstract
Extensive investigation of vertebrate striated muscle titin has yielded significant insight into its structure and function in striated muscle. We have begun to investigate other members of the titin protein family found in vertebrate smooth muscle and nonmuscle cells. Smooth and nonmuscle titins resemble striated muscle titin in molecular size and morphology but differ in their interactions with myosin II filaments and in the structural contexts in which they exist in vivo. Divergence of these titins from the muscle titin paradigm demonstrates the versatility of this remarkable family of giant proteins.
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References
Broschat KO, Stidwell RP, Burgess DR. Phosphorylation controls brush border motility by regulating myosin structure and association with the cytoskeleton. Cell 1983;35:561–71.
Drenkhahn D, Dermeitzel R. Organization of the actin filament cytoskeleton in the intestinal brush border: a quantitative and qualitative immunoelectron microscope study. J Cell Biol 1988;107:1037–48.
Eilertsen KJ, Kazmierski ST, Keller TCS, III. Cellular titin localization in stress fibers and interaction with myosin II filaments in vitro. J Cell Biol 1994;126:1201–10.
Eilertsen KJ, Kazmierski ST, Keller TCS, III. Interaction of α-actinin with cellular titin. Eur J Cell Biol 1997;74:361–64.
Eilertsen KJ, Keller TCS, III. Identification and characterization of two huge protein components of the brush border cytoskeleton: Evidence for a cellular isoform of titin. J Cell Biol 1992;119:549–57.
Gregorio CC, Granzier H, Sorimachi H, Labeit S. Muscle Assembly: a titanic achievement? Curr Opin Cell Biol 1999;11:18–25.
Hirokawa N, Keller TCS, Chasan R, Mooseker MS. Mechanisms of Brush Border Contractility Studied by the Quick-freeze, Deep-etch Method. J Cell Biol 1983;96:1325–36.
Hirokawa N, Tilney LG, Fujiwara K, Heuser JE. Organization of actin, myosin, and intermediate filaments in the brush border of intestinal epithelial cells. J Cell Biol 1982;94:425–43.
Keller TCS, Conzelman KA, Chasan R, Mooseker MS. The role of myosin in terminal web contraction in isolated intestinal epithelial brush borders. J Cell Biol 1985;100:1647–55.
Keller TCS, Mooseker MS. “Enterocyte cytoskeleton: its structure and function.” In Handbook of Physiology. Section 6: The gastrointestinal system 4th ed, M Field, RA Frizzell, eds. Bethesda, MD: American Physiological Society, 1991.
Keller TCS, III. Structure and function of titin and nebulin. Curr Opin Cell Biol 1995;7:32–38.
Labeit S, Gautel M, Lakey A, Trinick J. Towards a molecular understanding of titin. EMBO J 1992;11:1711–16.
Maruyama K. Connectin/titin, giant elastic protein of muscle. FASEB J 1997;11:341–45.
Maruyama K, Matsubara S, Natori R, Nonomura Y, Kimura S, Ohashi K, Murakami F, Handa S, Eguchi G. Connectin, an elastic protein of muscle: characterization and function. J Biochem(Tokyo) 1977;82:317–37.
Mayans O, van der Ven PF, Wilm M, Mues A, Young P, Fürst DO, Wilmanns M, Gautel M. Structural basis for activation of the titin kinase domain during myofibrillogenesis. Nature 1998;395:863–69.
Pavalko FM, Burridge K. Disruption of the actin cytoskeleton after microinjection of proteolytic fragments of a-actinin. J Cell Biol 1991;114:481–91.
Sorimachi H, Freiburg A, Kolmerer B, Ishiura S, Stier G, Gregorio CC, Labeit D, Linke WA, Suzuki K, Labeit S. Tissue-specific expression and alpha-actinin binding properties of the Z-disc titin: implications for the nature of vertebrate Z-discs. J Mol Biol 1997;270:688–95.
Trombitás K, Pollack GH. Elastic properties of the titin filament in the Z-line region of vertebrate striated muscle. J Muscle Res Cell Motil 1993;14:416–22.
Verkhovsky AB, Svitkina TM, Borisy GG. Myosin II filaments assemblies in the active lamella of fibroblasts: their morphogenesis and role in the formation of actin filament bundles. J Cell Biol 1995;131:989–1002.
Wang K, McClure J, Tu A. Titin: major myofibrillar components of striated muscle. Proc Natl Acad Sci USA 1979;76:3698–702.
Whiting A, Wardale J, Trinick J. Does titin regulate the length of muscle thick filaments? J Mol Biol 1989;205:263–67.
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Keller, T.C.S., Eilertsen, K., Higginbotham, M., Kazmierski, S., Kim, KT., Velichkova, M. (2000). Role of Titin in Nonmuscle and Smooth Muscle Cells. In: Granzier, H.L., Pollack, G.H. (eds) Elastic Filaments of the Cell. Advances in Experimental Medicine and Biology, vol 481. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4267-4_16
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DOI: https://doi.org/10.1007/978-1-4615-4267-4_16
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