Abstract
Random fragments of DNA were screened for their repetitive nature and hybridization affinity towards a single genome. Repetitive, genomic-specific probes were developed from diploid species such as Brassica rapa, B. nigra, and Sinapis alba. These types of probes have the ability to detect and quantify the amount of alien genome that is present in progeny of early backcross generations. Therefore, pland breeders can implement these probes by selecting a) lines that carry the desired alien phenotype and b) lines with the least amount of alien DNA, to reduce linkage drag.
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Somers, DJ., Zhou, Z., Bebeli, P.J., and Gustafson, J.P., 1996, Repetitive, genome-specific probes in wheat (Triticum aestivum L. em Thell.) amplified with minisatellite core sequences, Theor. Appl. Genet. 93:982–989.
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© 2000 Springer Science+Business Media New York
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Somers, D.J., Séguin-Swartz, G., Demmon, G., Danielson, J. (2000). Genome Specific DNA Probes in Crucifers. In: Gustafson, J.P. (eds) Genomes. Stadler Genetics Symposia Series. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4235-3_26
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DOI: https://doi.org/10.1007/978-1-4615-4235-3_26
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