Advertisement

A Role for Arabinogalactan-Proteins in Root Growth

  • Clare G. Steele-King
  • J. Paul Knox

Abstract

The synthetic phenylglycoside, β-glucosyl Yariv reagent (βGlcY), binds specifically to a subset of AGPs in Arabidopsis thaliana roots. When wild-type seedlings are germinated in media containing 30 μM βGlcY, a dramatic reduction in root and shoot growth, compared to control seedlings, is observed (Willats and Knox 1996). Short roots are a result of reduced cell elongation rather than reduced cell proliferation. We have used this βGlcY-phenotype in wild-type plants as a reference point in the screening of T-DNA and EMS-generated mutants for seedlings that are supersensitive or insensitive to βGlcY, or which show an altered pattern of βGlcY binding. The EMS-generated mutant, YSS1, has an apparently wild-type phenotype at 14 days. However, when YSS1 is germinated in the presence of βGlcY, the growth of the seedling is retarded to a much greater extent than wild-type seedlings, and the root is less than a millimetre in length at 14 days. This result with YSS1 is in contrast to wild-type seedlings grown in 30 μM βGlcY, where maximum root length is 8 mm. When YSS1 is transferred to medium without βGlcY, the mutant appears to recover a wild-type phenotype. This mutant, YSS1, is a very recent finding in our laboratory, and results are preliminary. It is possible, however, that YSS1 is a mutant that is supersensitive to βGlcY treatment. If this is the case, molecular studies of YSS1 are likely to yield important data regarding the βGlcY-binding subset of AGPs and their role in cell expansion.

Keywords

Cell Expansion Short Root Reduce Cell Proliferation Altered Pattern Maximum Root Length 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Reference

  1. Willats, W. G. T., and Knox, J. P., 1996, A role for arabinogalactan-proteins in plant cell expansion: Evidence from studies on the interaction of beta-glucosyl Yariv reagent with seedlings of Arabidopsis thaliana, Plant J. 9: 919–925.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Clare G. Steele-King
    • 1
  • J. Paul Knox
    • 1
  1. 1.Centre for Plant SciencesUniversity of LeedsLeedsUK

Personalised recommendations