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Are Gene Silencing Mutants Good Tools for Reliable Transgene Expression or Reliable Silencing of Endogenous Genes in Plants?

  • Philippe Mourrain
  • Christophe Béclin
  • Hervé Vaucheret
Part of the Genetic Engineering book series (GEPM, volume 22)

Abstract

Engineering new traits in plants by genetic transformation basically falls into two classes: i) introduction of sense transgenes that encode RNA and/or proteins adding a new structural or regulatory property to the plant, ii) introduction of antisense transgenes that encode RNA that interferes with the expression of endogenous genes or gene families. Many successful achievements involving transgenic plants expressing new traits have been obtained during the last 15 years. However, unexpected silencing of sense or antisense transgenes or simultaneous silencing of sense transgenes and homologous endogenous genes was also observed in several cases(1–5).

Keywords

Gene Silence Inverted Repeat Endogenous Gene Trans Gene Ethyl Methane Sulfonate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Philippe Mourrain
    • 1
  • Christophe Béclin
    • 1
  • Hervé Vaucheret
    • 1
  1. 1.Laboratoire de Biologie CellulaireINRAVersailles CedexFrance

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