Biosensors Based on “Wired” Peroxidases

  • Qiang Chen
  • Adam Heller
  • Gregory L. Kenausis

Abstract

Electrochemical and optical hydrogen peroxide sensors based on H202 producing enzyme-catalyzed reactions are commonly used in medical diagnostic assays.1-4 Electrochemical sensing usually allows use of small-volume and light-absorbing samples, such as whole blood, as well as facile integration into flow systems.’’’ Many assays involve an oxidase-catalyzed oxidation of a substrate by molecular oxygen in their first step, a reaction whereby an equimolar amount of H202 is formed:
$${\text{Substrate + }}{{\text{O}}_{{\text{2}}}}\xrightarrow{{{\text{Oxidase}}}}{\text{Product + }}{{\text{H}}_{{\text{2}}}}{{\text{O}}_{{\text{2}}}}$$
(1)
The H202 is then amperometrically measured in the second step through its electrooxidation to oxygen. This reaction proceeds at its transport-limited rate at a potential that depends on the nature of the surface of the electrode. On platinum, H202 is electrooxidized at + 700 mV (SCE).8,9

Keywords

Peroxide Lactate Platinum Immobilization NADPH 

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Copyright information

© Springer Science+Business Media New York 2000

Authors and Affiliations

  • Qiang Chen
    • 1
  • Adam Heller
    • 2
  • Gregory L. Kenausis
    • 3
  1. 1.Cygnus IncRedwood City
  2. 2.Department of Chemical EngineeringUniversity of Texas at AustinAustin
  3. 3.Laboratory for Surface Science and EngineeringETH ZurichZurichSwitzerland

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