Abstract
Sensitive detection systems (or sensors) are required to distinguish a wide range of substances. Sensors find application in the areas of biotechnology, physics, chemistry, medicine, aviation, and environmental control. Biosensor systems have different operating parameters, including stability, specificity, response time, selectivity, and regenerability. A better understanding of the mode of operation of these parameters would lead to increasing biosensor performance efficiency. Separation or the detection of reactants can be performed by the solid-phase immunoassay technique (antibody—antigen reaction) or by immobilizing enzymes on appropriate surfaces (enzyme—substrate reaction). There is a need to characterize the reactions occurring at the biosensor surface by paying attention to both the reaction and the sensing element (surface).
Keywords
- Fractal Dimension
- Fractal Analysis
- Tubulin Polymerization
- Total Internal Reflection Fluorescence
- Biosensor Surface
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References
Bluestein BI, Craig M, Slovacek R, et al. Evanescent wave immunosensors for clinical diagnostics. In: Wise D and Wingard Jr. LB, eds. Biosensors with Fiberoptics. Totowa, NJ: Humana Press, 1991, pp 181–223.
Eddowes MJ. Direct immunochemical sensing:basic chemical principles and fundamental limitations. Biosensors 1987/1988;3:1–15.
Kopelman R. Fractal reaction kinetics. Science 1988;241:1620–1626.
Pfeifer P, Obert M. Fractals:basic concepts and terminology. In: Avnir D, ed. The Fractal Approach to Heterogeneous Chemistry: Surfaces, Colloids, Polymers. New York: John Wiley & Sons, 1989, pp 112–123.
Seri-Levy A, Avnir D. Fractal analysis of surface geometry effects on catalytic reactions. Surf Sci 1991;248:258–270.
Sadana A, Beelaram A. A fractal analysis of antigen—antibody binding kinetics:biosensor applications. Biotechnol Progr 1994;10:291–298.
Sadana A, Beelaram A. Antigen—antibody diffusion-limited binding kinetics of biosensors: a fractal analysis. Biosens Bioelectron 1995;10:310–316.
Sadana A. Antigen—antibody binding kinetics for biosensors: the fractal dimension and the binding rate coefficient. Biotechnol Progr 1995;11:50–57.
Sadana A, Sii D. The binding of antigen by immobilized antibody: influence of a variable rate coefficient on external diffusional limitations. J Colloid Interf Sci 1992;151(1):166–177.
Sadana A, Sii D. Binding kinetics of antigen by immobilized antibody: influence of reaction order and external diffusional limitations. Biosens Bioelectron 1992;7:559–568.
Havlin S. Molecular diffusion and reaction. In: Avnir D, ed. The Fractal Approach to Heterogeneous Chemistry: Surfaces, Colloids, Polymers. New York: John Wiley & Sons, 1989, pp 251–269.
Piehler J, Brecht A, Gauglitz G. Affinity detection of low molecular weight analytes. Anal Chem 1996;68:139–143.
Sigmaplot (1993). Scientific Graphing Software, User’s Manual. Jandel Scientific, San Rafael, CA.
Domenici C, Schirone A, Celebre M, et al. Development of a TIRF immunosensor: modeling the equilibrium behavior of a competitive system. Biosens Bioelectron 1995;10:371–378.
Liliom K, Lehotzky A, Molnar A, et al. Characterization of tubulin-alkaloid interactions by enzyme-linked immunosorbent assay. Anal Biochem 1995;228:18–26.
Correia JJ. Pharmacol Ther 1991;52:127–147.
Douglas JF. How does surface roughness affect polymer-surface interactions? Macromolecules 1989:22:3707–3716.
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© 2000 Springer Science+Business Media New York
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Sadana, A. (2000). Analysis of the Kinetics of Antigen-Antibody Interactions and Fractal Dimension in Biosensors. In: Yang, V.C., Ngo, T.T. (eds) Biosensors and Their Applications. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4181-3_2
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DOI: https://doi.org/10.1007/978-1-4615-4181-3_2
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