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Bacterial Translocation from the Gastrointestinal Tract

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Part of the Advances in Experimental Medicine and Biology book series (AEMB,volume 473)

Summary

Bacterial translocation is defined as the passage of viable bacteria from the gastrointestinal (GI) tract to extraintestinal sites, such as the mesenteric lymph node complex (MLN), liver, spleen, kidney, and bloodstream. The three primary mechanisms promoting bacterial translocation in animal models are identified as: (a) disruption of the ecologic GI equilibrium to allow intestinal bacterial overgrowth, (b) increased permeability of the intestinal mucosal barrier, and (c) deficiencies in host immune defenses. These mechanisms can act in concert to promote synergistically the systemic spread of indigenous translocating bacteria to cause lethal sepsis. In animal models in which the intestinal barrier is not physically damaged, indigenous bacteria translocate by an intra-cellular route through the epithelial cells lining the intestines and then travel via the lymph to the MLN. In animal models exhibiting damage to the mucosal epithelium, indigenous bacteria translocate intercellularly between the epithelial cells to directly access the blood. Indigenous GI bacteria have been cultured directly from the MLN of various types of patients. Thus, evidence is accumulating that translocation of indigenous bacteria from the GI tract is an important early step in the pathogenesis of opportunistic infections originating from the GI tract.

Keywords

  • Hemorrhagic Shock
  • Latex Particle
  • Mesenteric Lymph Node
  • Intestinal Permeability
  • Bacterial Translocation

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  • Alexander, J.W., Gianotti, L., Pyles, T., Carey, M.A., and Babcock, G.F., 1990, Distribution and survival of Escherichia coli translocating from the intestines after thermal injury, Ann. Surg. 213:558–567.

    CrossRef  Google Scholar 

  • Ambrose, M.S., Johnson, M., Burdon, D.W., and Keighley, M.R.B., 1984, Incidence of pathogenic bacteria from mesenteric lymph nodes and ileal serosa during Crohn’s disease surgery, Brit J. Surg. 71:623–625.

    PubMed  CrossRef  CAS  Google Scholar 

  • Baker, J.W., Deitch, E.A., Berg, R., and Ma, L., 1987, Hemorrhagic shock impairs the mucosal barrier resulting in bacterial translocation from the gut and sepsis, Surg. Forum. 37:73–74.

    Google Scholar 

  • Baker, J.W., Deitch, E.A., Ma, L., and Berg, R., 1988, Hemorrhagic shock promotes the systemic translocation of bacteria from the gut, J. Trauma. 28:896–906.

    PubMed  CrossRef  CAS  Google Scholar 

  • Berg, R., 1995, Bacterial translocation from the gastrointestinal tract, Trends Microbiol. 3:149–154.

    PubMed  CrossRef  CAS  Google Scholar 

  • Berg, R., 1996, The indigenous gastrointestinal microflora, Trends Microbiol. 4:430–435.

    PubMed  CrossRef  CAS  Google Scholar 

  • Berg, R., 1998, Translocation of microbes from the intestinal tract, In: Medical importance of the normal microflora, Editor: Tannock, G, Chapman & Hall, London, p. 338–370.

    Google Scholar 

  • Berg, R., Berg, M., and Block, E., 1996, Comparison of translocation of Escherichia coli strains from the gastrointestinal tract, In: Germfree life and its ramifications, Editor: Hashimoto, K., et al., XII ISG Publishing Committee, Shiozawa, p. 53–58.

    Google Scholar 

  • Berg, R., Block, E., Berg, M., and Love, J., 1996, Translocation of latex particles from the gastrointestinal tract to the mesenteric lymph nodes, In: Germfree life and its ramifications, Editor: Hashimoto, K., et al., XII ISG Publishing Committee, Shiozawa. p. 47–51.

    Google Scholar 

  • Berg, R.D., 1980a, Inhibition of Escherichia coli translocation from the gastrointestinal tract by the normal cecal flora in gnotobiotic or antibiotic-decontaminated mice, Infect. Immun. 29:1073–1081.

    PubMed  CAS  Google Scholar 

  • Berg, R.D., 1980b, Mechanisms confining indigenous bacteria to the gastrointestinal tract, Amer. J. Clin. Nutr. 33:2472–2484.

    PubMed  CAS  Google Scholar 

  • Berg, R.D., 1981a, Factors influencing the translocation of bacteria from the gastrointestinal tract, In: Recent advances in germfree research, Editors: Sasaki, S., Ozawa, A., and Hashimoto, K., Tokai University Press, Tokyo, p. 411–418.

    Google Scholar 

  • Berg, R.D., 1981b, Promotion of the translocation of enteric bacteria from the gastrointestinal tracts of mice by oral treatment with penicillin, clindamycin, or metronidazole, Infect. Immun. 33:851–861.

    Google Scholar 

  • Berg, R.D., 1983a, Bacterial translocation from the gastrointestinal tracts of mice receiving immunosuppressive chemotherapeutic agents, Curr. Microbiol. 8:285–292.

    CrossRef  CAS  Google Scholar 

  • Berg, R.D., 1983b, Translocation of indigenous bacteria from the intestinal tract, In: The intestinal microflora in health and disease, Editor: Hentges, D, Academic Press, New York, p. 333–352.

    CrossRef  Google Scholar 

  • Berg, R.D., 1983c, The immune response to indigenous intestinal bacteria, In: The intestinal microflora in health and disease, Editor: Hentges, D, Academic Press, New York, p. 101–126.

    CrossRef  Google Scholar 

  • Berg, R.D., 1985, Bacterial translocation from the intestines, Exp. Animals. 34:1–16.

    CAS  Google Scholar 

  • Berg, R.D., 1992a, Bacterial translocation from the gastrointestinal tract, J. Med. 23:217–244.

    PubMed  CAS  Google Scholar 

  • Berg, R.D., 1992b, Translocation and the indigenous gut flora, In: Scientific basis of the probiotic concept, Editor: Fuller, R., Chapman & Hall, London, p. 55–85.

    Google Scholar 

  • Berg, R.D., 1992c, Translocation of enteric bacteria in health and disease. In: Gut-derived infectious toxic shock (GITS). A major variant of septic shock, Editor: Cottier, H. and Kraft, R., S. Karger AG, Basel, Switzerland, p. 44–65.

    Google Scholar 

  • Berg, R.D. and Garlington, A.W., 1979, Translocation of certain indigenous bacteria from the gastrointestinal tract to the mesenteric lymph nodes and other organs in a gnotobiotic mouse model, Infect. Immun. 23:403–411.

    PubMed  CAS  Google Scholar 

  • Berg, R.D. and Itoh, K., 1986, Bacterial translocation from the gastrointestinal tract-immunologic aspects, Microecol. Therapy. 16:131–145.

    Google Scholar 

  • Berg, R.D. and Owens, W.E., 1979, Inhibition of translocation of viable Escherichia coli from the gastrointestinal tract of mice by bacterial antagonism, Infect. Immun. 25:820–827.

    PubMed  CAS  Google Scholar 

  • Berg, R.D., Wommack, E., and Deitch, E.A., 1988, Immunosuppression and intestinal bacterial overgrowth synergistically promote bacterial translocation, Arch. Surg. 123:1359–1364.

    PubMed  CrossRef  CAS  Google Scholar 

  • Brathwaite, C., Rose, S., Nagele, R., Mure, A., O’Malley, K., and Garcia-Perez, E, 1993, Bacterial translocation occurs in humans after traumatic injury: evidence using immunofluorescence, J.Trauma. 34:586–590.

    PubMed  CrossRef  CAS  Google Scholar 

  • Carrico, C.J., Meakins, J.L., Marshall, J.C., Fry, D., and Maier, R.V., 1986, Multiple organ failure syndrome, Arch. Surg. 121:196–208.

    PubMed  CrossRef  CAS  Google Scholar 

  • Deitch, E.A. and Berg, R.D., 1987, Endotoxin but not malnutrition promotes bacterial translocation of the gut flora in burned mice, J. Trauma. 27:161–166.

    PubMed  CrossRef  CAS  Google Scholar 

  • Deitch, E.A., Bridges, W.R., Ma, L., Berg, R., Specian, R., and Granger, N., 1989a, Role of xanthine oxidase and neutrophil generated oxy radicals in shock-induced bacterial translocation, J. Trauma. 29:1027.

    CrossRef  Google Scholar 

  • Deitch, E.A., Ma, L., Ma, J.W., Grisham, M.N.G., Specian, R., and Berg, R., 1989b, Inhibition of endotoxininduced bacterial translocation in mice, J. Clin. Invest. 84:36–42.

    PubMed  CrossRef  CAS  Google Scholar 

  • Deitch, E.A., Winterton, J., and Berg, R., 1987, Effect of starvation, malnutrition, and trauma on the GI tract flora and bacterial translocation, Arch. Surg. 122:1019–1025.

    PubMed  CrossRef  CAS  Google Scholar 

  • Deitch, E.A., Bridges, W., Baker, J., Ma, J.-W., Ma, L., Grisham, M., Granger, N., Specian, R., and Berg, R., 1988, Hemorrhagic shock-induced bacterial translocation is reduced by xanthine oxidase inhibition or inactivation, Surgery. 104:191–198.

    PubMed  CAS  Google Scholar 

  • Donnenberg, M., Newman, B., Utsalo, S., Trifillis, A., Hebel, I, and Warren, J., 1994, Internalization of Escherichia coli into human kidney epithelial cells: Comparison of fecal and pyelonephritis-associated strains, J. Infect. Dis. 169:831–838.

    PubMed  CrossRef  CAS  Google Scholar 

  • Feltis, B., Jechorek, R., Erlandsen, S., and Wells, C., 1994, Bacterial translocation and lipopolysaccharideinduced mortality in genetically macrophage-déficient op/op mice, Shock. 2:29–33.

    PubMed  CrossRef  CAS  Google Scholar 

  • Fuller, K.G. and Berg, R.D., 1985, Inhibition of bacterial translocation from the gastrointestinal tract by nonspecific stimulation, In: Microflora control and its application to the biomédical sciences, Editor: Wostmann, B, S, Alan R. Liss, Inc., New York., p. 195–198.

    Google Scholar 

  • Gautreaux, M., Deitch, E., and Berg, R.D., 1994a, Bacterial translocation from the gastrointestinal tract to various segments of the mesenteric lymph node complex, Infect. Immun. 62:2132–2134.

    PubMed  CAS  Google Scholar 

  • Gautreaux, M., Deitch, E., and Berg, R.D., 1994b, T-cells in the host defense against bacterial translocation from the gastrointestinal tract, Infect. Immun. 62:2874–2884.

    PubMed  CAS  Google Scholar 

  • Gautreaux, M.D., Deitch, E.A., and Berg, R.D., 1990, Immunological mechanisms preventing bacterial translocation from the gastrointestinal tract, Microecol. Ther. 20:31–34.

    Google Scholar 

  • Gautreaux, M.D., Deitch, E.A., and Berg, R.D., 1993, T lymphocytes in host defense against bacterial trans-location from the gastrointestinal tract, Infect. Immun. 62:2874–2884.

    Google Scholar 

  • Krause, W., Matheis, H., and Wulf, K., 1969, Fungaemia and funguria after oral administration of Candida albicans, Lancet. 1:598–600.

    PubMed  CrossRef  CAS  Google Scholar 

  • Ma, L., Specian, R.D., Berg, R.D., and Deitch, E.A., 1989, Effects of protein malnutrition and endotoxin on the intestinal mucosal barrier to the translocation of indigenous flora in mice, J. Parenteral Enterai Nutr. 13:572–578.

    CrossRef  Google Scholar 

  • Maejima, K., Deitch, E.A., and Berg, R.D., 1984a, Bacterial translocation from the gastrointestinal tract of rats receiving thermal injury, Infect. Immun. 43:6–10.

    PubMed  CAS  Google Scholar 

  • Maejima, K., Deitch, E.A., and Berg, R.D., 1984b, Promotion by burn stress of the translocation of bacteria from the gastrointestinal tracts of mice, Arch. Surg. 119:166–172.

    PubMed  CrossRef  CAS  Google Scholar 

  • Maejima, K., Shimoda, K., and Berg, R.D., 1984, Assessment of mouse strain on bacterial translocation from the gastrointestinal tract, Exp. Animals. 33:345–349.

    CAS  Google Scholar 

  • Michetti, P., Mahan, M., Slauch, J., Mekalanos, J., and Neutra, M., 1992, Monoclonal secretory immunoglobulin A protects mice against oral challenge with the invasive pathogen Salmonella typhimurium, Infect. Immun. 60:1786–1792.

    PubMed  CAS  Google Scholar 

  • Moore, F., Moore, E., Poggetti, R., and Read, R., 1992, Postinjury shock and early bacteremia, Arch. Surg. 127:893–898.

    PubMed  CrossRef  CAS  Google Scholar 

  • Moore, F.A., Moore, E.E., Poggetti, R., McAnena, O.J., Peterson, V.M., Abernathy, CM., and Parsons, P.E., 1991, Gut bacterial translocation via the portal vein: A clinical perspective with major torso trauma, J. Trauma. 31:629–638.

    PubMed  CrossRef  CAS  Google Scholar 

  • Morehouse, J., Specian, R., Stewart, J., and Berg, R.D., 1986, Promotion of the translocation of indigenous bacteria of mice from the GI tract by oral ricinoleic acid, Gastroenterology. 91:673–682.

    PubMed  CAS  Google Scholar 

  • Nolan, J., 1981, Endotoxin, reticuloendothelial function, and liver injury, Hepatology. 1:458–465.

    PubMed  CrossRef  CAS  Google Scholar 

  • Owens, W.E. and Berg, R.D., 1980, Bacterial translocation from the gastrointestinal tract of athymic (nu/nu) mice, Infect. Immun. 27:461–467.

    PubMed  CAS  Google Scholar 

  • Owens, W.E. and Berg, R.D., 1982, Bacterial translocation from the gastrointestinal tracts of thymectomized mice, Curr. Microbiol. 7:169–174.

    CrossRef  Google Scholar 

  • Parks, D.A., Bulkley, G.B., Granger, D.N., Hamilton, S.R., and McCord, J.M., 1982, Ischémic injury in the cat small intestine: Role of Superoxide radicals, Gastroenterology. 82:9–15.

    PubMed  CAS  Google Scholar 

  • Peitzman, A.B., Udekwu, A.O., Ochoa, J., and Smith, S., 1991, Bacterial translocation in trauma patients, J. Trauma. 31:1083–1087.

    PubMed  CAS  Google Scholar 

  • Penn, R.L., Maca, R.D., and Berg, R.D., 1986, Leukemia promotes the translocation of indigenous bacteria from the gastrointestinal tract to the mesenteric lymph node and other organs, Microecol. Ther. 15:85–91.

    Google Scholar 

  • Ravin, H.A. and Fine, J., 1962, Biological implications of intestinal endotoxaemia, Fed. Proc. 21:65–68.

    PubMed  CAS  Google Scholar 

  • Reed, L., Martin, M., Manglano, R., Newson, B., Kocka, F., and Barrett, J., 1994, Bacterial translocation following abdominal trauma in humans, Cir. Shock. 42:1–6.

    CAS  Google Scholar 

  • Rush, B.F., Sori, A.J., Murphy, T.F., Smith, S., Flanagan, J.J., and Machiedo, G.W., 1988, Endotoxemia and bacteremia during hemorrhagic shock: the link between trauma and sepsis?, Ann. Surg. 207:549–554.

    PubMed  CrossRef  Google Scholar 

  • Sedman, P., Macfie, J., Sagar, P., Mitchell, C., May, J., Mancey-Jones, B., and Johnstone, D., 1994, The prevalence of gut translocation in humans, Gastroenterology. 107:643–649.

    PubMed  CrossRef  CAS  Google Scholar 

  • Steffen, E.K. and Berg, R.D., 1983, Relationship between cecal population levels of indigenous bacteria and translocation to the mesenteric lymph nodes., Infect. Immun. 39:1252–1259.

    PubMed  CAS  Google Scholar 

  • Steffen, E.K., Berg, R.D., and Deitch, E.A., 1988, Comparison of the translocation rates of various indigenous bacteria from the gastrointestinal tract to the mesenteric lymph nodes, J. Infect. Dis. 157:-1032–1037.

    PubMed  CrossRef  CAS  Google Scholar 

  • Tancrede, C.H. and Andremont, A.O., 1985, Bacterial translocation and gram-negative bacteremia in patients with hematological malignancies, J. Infect. Dis. 152:99–103.

    PubMed  CrossRef  CAS  Google Scholar 

  • Vincent, P., Colombel, XE, Lescut, D., Fournier, L., Savage, C., Cortot, A., Quandalle, P., Vankemmel, M., and Leclerc, H., 1988, Bacterial translocation in patients with colorectal cancer, J. Infect. Dis. 158:-1395–1396.

    PubMed  CrossRef  CAS  Google Scholar 

  • Wells, C., Ferrieri, P., Weisdorf, D., and Rhame, F., 1987, (I do not have) The importance of surveillance stool cultures during episodes of severe neutropenia, Infect. Control. 8:317–319.

    PubMed  CAS  Google Scholar 

  • Wells, C.L., Maddus, M.A., and Simmons, R.L., 1987, The role of the macrophage in the translocation of intestinal bacteria, Arch. Surg. 122:48–53.

    PubMed  CrossRef  CAS  Google Scholar 

  • Winner, L., III, Mack, J., Weltzin, R., Mekalanos, J., Kraehenbuhl, X-P, and Neutra, M., 1991, New model for analysis of mucosal immunity: Intestinal secretion of specific monoclonal immunoglobulin A from hybridoma tumors protects against Vibrio cholerae infection, Infect. Immun. 59:977–982.

    PubMed  CAS  Google Scholar 

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Berg, R.D. (1999). Bacterial Translocation from the Gastrointestinal Tract. In: Paul, P.S., Francis, D.H. (eds) Mechanisms in the Pathogenesis of Enteric Diseases 2. Advances in Experimental Medicine and Biology, vol 473. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4143-1_2

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  • DOI: https://doi.org/10.1007/978-1-4615-4143-1_2

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