Analytical procedures

  • F. D. Gunstone
Chapter

Abstract

Tests for describing various properties of a sample of oil or fat are part of the assessment of its commercial value or authenticity. They are concerned with the ‘quality’ of the oil rather than with its ‘chemical nature’ though some tests provide pointers to composition. For example, iodine value is an indication of the average unsaturation of the acyl chains. In some cases the chemical reactions underlying the tests are not fully understood and empirical values are obtained. The results are only reliable and of value for comparison purposes, when the tests are carried out carefully by standardized methods which have been worked out and approved by groups of analysts.

Keywords

High Performance Liquid Chromatography Iodine Aldehyde MeOH Choline 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Bibliography

  1. Bligh, E.G. and W.J. Dyer (1959) A rapid method of total lipide extraction and purification, Can. J. Biochem. Physiol., 37, 911–917.CrossRefGoogle Scholar
  2. Blomberg, L.G. and M. Demibuker (1994) Analysis of triacylglycerols by argentation supercritical fluid chromatography, in Developments in the Analysis of Lipids (eds J.H.P. Tyman and M.H. Gordon), Royal Society of Chemistry, Cambridge.Google Scholar
  3. Brockerhoff, H. (1971) Stereospecific analysis of triglycerides, Lipids, 6, 942–956.CrossRefGoogle Scholar
  4. Christie, W.W. (1987) High-performance Liquid Chromatography and Lipids, Pergamon, Oxford.Google Scholar
  5. Christie, W.W. (1987) A stable silver-loaded column for the separation of lipids by highperformance liquid chromatography, J. High Res. Chromatogr. Commun., 10, 148–150.CrossRefGoogle Scholar
  6. Christie, W.W. (1989) Gas Chromatography and Lipids, The Oily Press, Ayr.Google Scholar
  7. Christie, W.W. (1989) Silver ion chromatography using solid-phase extraction columns packed with a bonded-sulphonic acid phase, J. Lipid Res., 30, 1471–1473.Google Scholar
  8. Christie, W.W. (ed.) (1992) Advances in Lipid Methodology — One, The Oily Press, Ayr.Google Scholar
  9. Christie, W.W. (ed.) (1993) Advances in Lipid Methodology — Two, The Oily Press, Dundee.Google Scholar
  10. Christie, W.W. (1994) High performance liquid chromatography of chiral lipids, in Developments in the Analysis of Lipids (eds J.H.P. Tyman and M.H. Gordon) Royal Society of Chemistry, Cambridge.Google Scholar
  11. Folch, J., M. Lees and G.H.S. Stanley (1975) A simple method for the isolation and purification of total lipides from animal tissue, J. Biol. Chem., 226, 497–509.Google Scholar
  12. Gunstone, F.D. et al. (eds) (1994) The Lipid Handbook, 2nd edn, Chapman and Hall, London.Google Scholar
  13. Hamilton, RJ. and J.B. Rosseil (eds) (1986) Analysis of Oils and Fats, Elsevier Applied Science, London.Google Scholar
  14. Hammond, E. (1993) Chromatography for the Analysis of Lipids, CRC, Boca Raton.Google Scholar
  15. Itabashi, Y. and T. Takagi (1986) High performance liquid Chromatographic separation of monoacylglycerol enantiomers of a chiral stationary phase, Lipids, 21, 413–416.CrossRefGoogle Scholar
  16. Laakso, P. and W.W. Christie (1990) Chromatographic resolution of ehiral diacylglycerol derivatives: potential in the stereospecific analysis of triacyl-sn-glycerols, Lipids, 25, 349–353.CrossRefGoogle Scholar
  17. Laakso, P. and W.W. Christie (1991) Combination of silver ion and reversed-phase high-performance liquid chromatography in the fractionation of herring oil triacylglycerols, J. Amer. Oil Chem. Soc., 68, 213–223.CrossRefGoogle Scholar
  18. Myher, J.J. and A. Kuksis (1979) Stereospecific analysis of triacylglycerols via racemic phosphatidylcholines and phospholipase C, Can. J. Biochem., 57, 117–124.CrossRefGoogle Scholar
  19. Robbelen, G. et al. (eds) (1989) Oil Crops of the World — Their Breeding and Utilisation, McGraw-Hill, New York.Google Scholar
  20. Rossell, J.B. and J.L.R. Pritchard (eds) (1991) Analysis of Oilseeds, Fats, and Fatty Foods, Elsevier Applied Science, London.Google Scholar
  21. Sassano, G.J. and Jeffrey, B.S.J. (1993) J. Amer. Oil Chem Soc., 70, 1111–1114.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1996

Authors and Affiliations

  • F. D. Gunstone
    • 1
  1. 1.Lipid Chemistry Unit School of ChemistryUniversity of St AndrewsFifeUK

Personalised recommendations