Advertisement

Epidermal Dendritic Cells in Aged C57BL/6 J MICE

  • Eli Sprecher
  • Leonard D. Shultz
  • Yechiel Becker
Part of the Developments in Medical Virology book series (DIMV, volume 7)

Abstract

Aging is known to affect the immune function and structural integrity of the skin. The density and function of epidermal dendritic cell (DC) populations was investigated in old mice. Langerhans cell (LC) and Thy-1+ dendritic epidermal cell (Thy-1+DEC) density was found to decrease with age. This decrease correlated with an impairment in epidermal cell function as assessed in vitro by the skin-lymphocyte reaction assay and by measuring the ability of epidermal cell suspensions to stimulate the proliferation of sensitized T cells in the presence of the sensitizing antigen. However, the ability of LC to transport antigens from the skin to the draining lymph nodes was found in vivo to be normal in old mice as compared with young mice. Normal numbers of interdigitating dendritic cells (IDC) were recovered from the lymph nodes of old mice. Bone marrow from old and young donors was transplanted into young irradiated recipients. Results from these experiments indicated that low LC density in the skin of old mice probably results from impaired capacity of LC progenitor cells in the bone marrow of old mice to home into and repopulate the epidermis. Studies with herpes simplex virus type 1 (HSV-1) showed that old mice were markedly more susceptible to HSV-1 infection by the intraperitoneal or footpad routes of inoculation as compared with young mice. Systemic administration of thymosin α1 to old mice partially protected them from a lethal challenge with the virus. The higher suceptibility of old mice to HSV-1 infection was correlated with an impaired capacity to mobilize immune cells in the draining lymph nodes and with the absence, in old mice, of an increase in LC numbers following infection, which is usually seen in young mice, infected with HSV-1. In contrast, a normal increase in the numbers of IDC in the draining lymph nodes was observed in old mice. Systemic administration of thymosin α1 to old mice partially restores their ability to mobilize immune cells in the draining lymph nodes.

Keywords

Drain Lymph Node Young Mouse Primary Immunodeficiency Disease Epidermal Sheet Tris Maleate Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Lavker R.M., Zheng P. and Dong G., J. Invest. Dermatol, 88:44s–51s, 1987.PubMedCrossRefGoogle Scholar
  2. 2.
    Sauder D.N., Stanulis-Praeger B.M. and Gilchrest B.A., Arch. Dermatol. Res., 280:71–76, 1988.PubMedCrossRefGoogle Scholar
  3. 3.
    Gilchrest B.A., Blog F.B., Szabo G., J. Invest. Dermatol., 73:141–143, 1979.PubMedCrossRefGoogle Scholar
  4. 4.
    Gilchrest B.A., Murphy G.F. and Soter N.A., J. Invest. Dermatol, 79:85–88, 1982.PubMedCrossRefGoogle Scholar
  5. 5.
    Makinodan T. and Kay M.M.B., Adv. Immunol, 29:287–331, 1980.PubMedCrossRefGoogle Scholar
  6. 6.
    Horan H.A. and Fox R.A., Mech. Ageing Dev., 26:165–181, 1984.PubMedCrossRefGoogle Scholar
  7. 7.
    Menon M., Jaroslov B.N. and Koesterer R., J. Gerontol, 29:499–505, 1974.PubMedCrossRefGoogle Scholar
  8. 8.
    Matour D., Melnicoff M., Kaye D. and Murasko D.M., Clin. Immunol Immunopathol., 50:82–99, 1989.PubMedCrossRefGoogle Scholar
  9. 9.
    Chopra R.K., Nagel J.E., Chrest F.J. and Adler W.H., Clin. Exp. Immunol, 70:456–462, 1987.PubMedGoogle Scholar
  10. 10.
    Ershler W.B., Robbins D.L., Moore A.L. and Hebert J.C., Exp. Gerontol, 21:45, 1986.PubMedCrossRefGoogle Scholar
  11. 11.
    Konen T.G., Smith G.S. and Walford R.L., J. Immunol, 110:1216–1221, 1973.PubMedGoogle Scholar
  12. 12.
    Chang M.P., Makinodan T., W.J. Peterson and B.L. Strehler, J. Immunol, 129:2426–2430, 1982.PubMedGoogle Scholar
  13. 13.
    Nagel J.E., Chopra R.K., Chrest F.J., McCoy M.T., Schneider E.L., Holbrock N.J. and Adler W.H., J. Clin. Invest, 81:1096–1102, 1988.PubMedCrossRefGoogle Scholar
  14. 14.
    Inamizu T., Chang M.P. and Makinodan T., Immunology, 55:447–455, 1985.PubMedGoogle Scholar
  15. 15.
    Krishnaraj R. and Blanford G., Clin. Immunol. Immunopathol, 45:268–285, 1987.PubMedCrossRefGoogle Scholar
  16. 16.
    Brouwer A. and Knook D.L., Mech. Ageing Dev., 21:205–228, 1983.PubMedCrossRefGoogle Scholar
  17. 17.
    Makinodan T., James S.J., Inamizu T. and Chang M., Gerontology, 30:279–289, 1984.PubMedCrossRefGoogle Scholar
  18. 18.
    Finger H., Heymer B., Wirsing von König C.-H. and Emmerling P., Gerontology, 28:223–232, 1982.PubMedCrossRefGoogle Scholar
  19. ai]19.
    Heidrick M.L., Gerontology, 12:28–34, 1972.Google Scholar
  20. 20.
    Komatsubara S., Cinader B. and Muramatsu S., Scand. J. Immunol, 24:517–525, 1986.PubMedCrossRefGoogle Scholar
  21. 21.
    Price G.B. and Makinodan T., J. Immunol, 108:403–412, 1972.PubMedGoogle Scholar
  22. 22.
    Hirokawa K., Sato K. and Makinodan T., Clin. Immunol. Immunopathol, 22:297–304, 1982.PubMedCrossRefGoogle Scholar
  23. 23.
    Butenko G.M. and Gubrii I.B., Exp. Gerontol, 15:605–610, 1980.PubMedCrossRefGoogle Scholar
  24. 24.
    Astle C.M. and Harrison D.E., J. Immunol, 132:673–677, 1984.PubMedGoogle Scholar
  25. 25.
    Tsuda T., Kim Y.T., Siskind G.W. and Weksler M.E., Proc. Natl. Acad. Sci. USA, 85: 1169–1173, 1988.PubMedCrossRefGoogle Scholar
  26. 26.
    Hirokawa K., Kubo S., Utsuyama M., Kuraschima C. and Sado T., Cell. Immunol., 100:443–451, 1986.PubMedCrossRefGoogle Scholar
  27. 27.
    Lipschitz D.A., Kodetthoor B.U. and Boxer L.A., Blood, 70:1131–1135, 1987.PubMedGoogle Scholar
  28. 28.
    Zatz M.M. and Goldstein A.L., Gerontology, 31:263–277, 1985.PubMedCrossRefGoogle Scholar
  29. 29.
    Gottesman S.R.S., Edington J.M. and Thorbecke G.J., J. Immunol., 140:1783–1790, 1988.PubMedGoogle Scholar
  30. 30.
    Doria G., Mancini C., Frasca D. and Adorini L., J. Immunol., 139:1419–1425, 1987.PubMedGoogle Scholar
  31. 31.
    Meredith P.J. and Walford R.L., Mech. Ageing Dev., 9:61–77, 1979.PubMedCrossRefGoogle Scholar
  32. 32.
    Breatnach S.M., Br. J. Dermatol., 119:463–469, 1988.CrossRefGoogle Scholar
  33. 33.
    Tschachler E., Shuler G., Hutterer J., Leibl H., Wolff K. and Stingl G., J. Invest. Dermatol., 81:282–285, 1983.PubMedCrossRefGoogle Scholar
  34. 34.
    Bergstresser P.R., Tigelaar R.E., Dees J.H. and Streilein J.W., J. Invest. Dermatol., 81:286–288, 1983.PubMedCrossRefGoogle Scholar
  35. 34a.
    Kraal S.I., Breel M., Janse M., and Bruin G., J. Exp. Med., 163:981–997, 1986.PubMedCrossRefGoogle Scholar
  36. 35.
    Macatonia S.E., Edwards A.J. and Knight S.E., Immunology, 59:509–514, 1986.PubMedGoogle Scholar
  37. 36.
    Baker K.W. and Habowsky J.E.J., J. Invest. Dermatol., 80:104–107, 1983.PubMedCrossRefGoogle Scholar
  38. 37.
    Mackenzie I.C. and Squier C.A., Br. J. Dermatol., 92:523–533, 1975.PubMedCrossRefGoogle Scholar
  39. 38.
    Nordlund J.J. and Ackles A., Tissue Antigens, 17:217–225, 1981.PubMedCrossRefGoogle Scholar
  40. 39.
    Knight S.C., Mertin J., Stackpole A and Clark J., Proc. Natl. Acad. Sci., 80:6032–6035, 1983.PubMedCrossRefGoogle Scholar
  41. 40.
    Walford R.L., J. Immunol., 117:352–353, 1976.PubMedGoogle Scholar
  42. 41.
    Sprecher E., Becker Y., Kraal G., Hall E., Harrison D. and Shultz L.D., J. Invest. Dermatol., 94:247–253, 1990.PubMedCrossRefGoogle Scholar
  43. 42.
    Macatonia S.E., Knight S.E., Edwards A.J. Griffiths S. and Fryer P., J. Exp. Med., 166:1654–1667, 1987.PubMedCrossRefGoogle Scholar
  44. 43.
    Thomas W.R., Edwards A.J., Watkins M.C. and Asherson G.L., Immunology, 39:21–27, 1980.PubMedGoogle Scholar
  45. 44.
    McDermott M.R., Smiley J.R., Leslie P., Brais J., Rudzroga H.E. and Bienenstock J., J. Virol., 51:747–753, 1984.PubMedGoogle Scholar
  46. 45.
    Sprecher E. and Becker Y., Arch. Virol., 91:341–349, 1986.PubMedCrossRefGoogle Scholar
  47. 46.
    Goldstein A.L., Shulof R.S., Naylor P.H. and Hall N.R., Med. Oncol. Tumor Pharmacother., 3:211–221, 1986.PubMedGoogle Scholar
  48. 47.
    Zatz M.M. and Goldstein A.L., Gerontology, 31:263–277, 1985.PubMedCrossRefGoogle Scholar
  49. 48.
    Frasca D., Garavini M. and Doria G., Cell. Immunol., 72:384–391, 1982.PubMedCrossRefGoogle Scholar
  50. 49.
    Ishitsuka H., Umeda Y., Nakamura J. and Yagi Y., Cancer Immunol. Immunother., 14:145–150, 1983.PubMedCrossRefGoogle Scholar
  51. 50.
    Thiers B.H., Maize J.C., Spicer S.S. and Cantor A.B., J. Invest. Dermatol., 82:223–226, 1984.PubMedCrossRefGoogle Scholar
  52. 51.
    Belsito D.V., Baer R.L. and Thorbecke G.J., in:Primary Immunodeficiency Diseases, pp. 253–268, Eibl M.M. and Rosen F.S. eds., Elsevier Science Publishers, Amsterdam, 1986.Google Scholar
  53. 52.
    Belsito D.V., Dersarkissian R.M., Thorbecke G.J., Baer R.L., Arch Dermatol. Res., 279:76s, 1987.CrossRefGoogle Scholar
  54. 53.
    Gu S.-Q, Sakuma M., Naito S., Baba T. and Uyeno K.-I., Acta Derm. Venereol., 69:1–5, 1989.PubMedGoogle Scholar
  55. 54.
    Phair J.P., Hsu C.S. and Hsu Y.L., Ciba Found. Sym., 134:143–155, 1988.Google Scholar
  56. 55.
    Mor V., Ciba Found. Sym., 134:160–176, 1988.Google Scholar
  57. 56.
    Dalakas M.C. and Trapp B.D., Ann. Neurol., 19:349–355, 1986.PubMedCrossRefGoogle Scholar
  58. 57.
    Shiohara T., Moriya N., Saizawa K.M. T. and Nagashima M., Arch. Dermatol. Res., 280:33–38, 1988.PubMedCrossRefGoogle Scholar
  59. 58.
    Berman B., Duncan M., Smith B., Ziboh V.A. and Palladino M., J. Invest. Dermatol, 84:54–58, 1985.PubMedCrossRefGoogle Scholar
  60. 59.
    Rheins L.A. and Nordlund J.J., J. Immunol, 136:867–876, 1986.PubMedGoogle Scholar
  61. 60.
    Shiohara T., Koyobashi M., Narimatsu H. and Nagashima M., Arch. Dermatol Res., 279:198–203, 1987.PubMedCrossRefGoogle Scholar
  62. 61.
    Tsujitani S., Okamura T., Baba H., Korenaga D., Haraguchi M. and Sugimachi K., Cancer, 61:1749–1753, 1988.PubMedCrossRefGoogle Scholar
  63. 62.
    Larsen C.G., Ternowitz T., Larsen F.G. and Thestrup-Pedersen K., Arch. Dermatol Res., 280:83–89, 1988.PubMedCrossRefGoogle Scholar
  64. 63.
    Gervais F., Patel P. and Skamene E., J. Gerontol, 43:B152–B156, 1988.PubMedCrossRefGoogle Scholar
  65. 64.
    Szewczuk M.R., Campbell R.J. and Jung L.K., J. Immunol, 126:2200–2204, 1981.PubMedGoogle Scholar
  66. 65.
    Ernst D.N., Weigle W.O. and Thoman M.L., J. Immunol, 138:26–31, 1987.PubMedGoogle Scholar
  67. 66.
    Romani N., Koide S., Crowley M., Witmer-Pack M., Livingstone A.M., Fathman C.G., Inaba K. and Steinman R., J. Exp. Med., 169:1169–1174, 1989.PubMedCrossRefGoogle Scholar
  68. 67.
    Schuler G. and Steinman R.M., J. Exp. Med., 161:526–546, 1985.PubMedCrossRefGoogle Scholar
  69. 68.
    Kay M.M.B., Aging, 11:33–77, 1980.Google Scholar
  70. 69.
    Harrison D.E., Astle C.M. and Stone M., J. Immunol, 142:3833–3840, 1989.PubMedGoogle Scholar
  71. 70.
    Williams L.H., Udupa K.B. and Lipschitz D.A., Exp. Hematol, 14:827–832, 1986.PubMedGoogle Scholar
  72. 71.
    Siskind G.W., Aging, 31:235–241, 1988.Google Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Eli Sprecher
    • 1
  • Leonard D. Shultz
    • 1
    • 2
  • Yechiel Becker
    • 1
  1. 1.Department of Molecular Virology, Faculty of MedicineHebrew UniversityJerusalemIsrael
  2. 2.The Jackson LaboratoryBar HarborUSA

Personalised recommendations