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Detection of Langerhans Cells with Monoclonal Antibodies

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Skin Langerhans (Dendritic) Cells in Virus Infections and AIDS

Part of the book series: Developments in Medical Virology ((DIMV,volume 7))

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Abstract

We prepared a mouse monoclonal antibody that reacts specifically to human Langerhans cells (LC). The protein recognized by this antibody was mainly in the membranes of Birbeck granules and related structures. Using this antibody (Lag), we could identify LC in normal various tissues; these cells were in the skin, stratified squamous mucosal epithelia, lymph nodes, and the thymus. Lag did not react with monocytes, tissue macrophages, lymphoid dendritic cells, follicular dendritic cells, or interdigitating cells. The antigen purified with this antibody was a heterogenously glycosylated protein of Mr 40, 000 without interchain disulfide bonds. In Letterer-Siwe disease, both lymph nodes and lesional skin contained abundant Lag-positive cells. By two-dimensional gel electrophoresis, antigenic subtances in the lymph nodes of patients with Letterer-Siwe disease were found to have the same molecular weight of 40, 000 dalton and isoelectric points extending from 4.7 to 6.5 as those in normal human skin and lymph nodes. Our results support the contention that Letterer-Siwe disease is a proliferative disorder of Langerhans cells. This antibody (Lag) may be useful for identifying normal or abnormal LC in various human tissues, and for studying the origin and fate of Birbeck granules of LC.

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Authors and Affiliations

  1. Department of Dermatology, Faculty of Medicine, Kyoto University, Kyoto, 606, Japan

    M. Kashihara-Sawami

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  1. M. Kashihara-Sawami
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  2. S. Imamura
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Editors and Affiliations

  1. Hebrew University, Jerusalem, Israel

    Yechiel Becker

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© 1991 Springer Science+Business Media New York

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Kashihara-Sawami, M., Imamura, S. (1991). Detection of Langerhans Cells with Monoclonal Antibodies. In: Becker, Y. (eds) Skin Langerhans (Dendritic) Cells in Virus Infections and AIDS. Developments in Medical Virology, vol 7. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3942-1_3

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  • DOI: https://doi.org/10.1007/978-1-4615-3942-1_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6759-8

  • Online ISBN: 978-1-4615-3942-1

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