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Trans-acting factors involved in species- specificity and control of mouse ribosomal gene transcription

  • Andreas Schnapp
  • Horst Rosenbauer
  • Ingrid Grummt
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 7)

Abstract

Faithful and efficient transcription initiation at the mouse ribosomal gene promoter requires besides RNA polymerase I (pol I) four polypeptide toms-acting factors, termed TIF-IA, TIF-IB, TIF-IC, and mUBF. We have partially purified these proteins from cultured Ehrlich ascites cells and show that in the presence of TIF-IA and TIF-IB, pol I directs very low amounts of specific transcripts. Neither TIF-IC nor mUBF on their own significantly stimulate the efficiency of template utilization. However, both factors together strongly activate transcription. Interestingly, factor TIF-IB - the murine homologue of human SL1 - fails to program a human extract to transcribe the murine template, but requires its homologous RNA polymerase I. This finding implicates that not only some rDNA transcription factors but also pol I exhibits species-specific differences. The growth-related factor TIF-IA, on the other hand, stimulates both mouse and human rDNA transcription. This regulatory factor whose amount or activity fluctuates according to the proliferation rate of the cells, is functionally inactivated by antibodies against cdc2 protein kinase. This result together with the observation that transcription is stimulated by ATP-γS, an ATP analogue which is a substrate for protein kinases but not for protein phosphatases, strongly suggests that post-translational protein modification is involved in rDNA transcription regulation.

Key words

RNA polymerase I transcription factors signal transduction protein phosphorylation cdc2 protein kinase 

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Copyright information

© Springer Science+Business Media Dordrecht 1991

Authors and Affiliations

  • Andreas Schnapp
    • 1
  • Horst Rosenbauer
    • 1
  • Ingrid Grummt
    • 1
  1. 1.Institute of Cell and Tumor BiologyGerman Cancer Research CenterHeidelbergGermany

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