Mechanism of the Cytotoxic Interaction between ET-18-OMe and Heat
Ether lipid analogues of platelet-activating factor have been shown to kill cancer cells either alone or in combination with other cancer chemotherapeutic agents (1–3). Recently, we have shown that one of the ether lipid analogues, ET-18-OMe1, and 44°C hyperthermia interact supra-additively to kill BG-1 human ovarian carcinoma cells at concentration ≤0.5 μM of ET-18-OMe and additively at higher concentrations (0.5 to 2.0 μM) of ET-18-OMe (4). The mechanism of this cytotoxic interaction of ET-18-OMe and heat is presently unknown. In this study, we examine flow cytometrically two parameters, cell cycle distribution and nuclear protein content, that are altered after heat treatment to determine if they are involved in the mechanism governing the interaction of heat and ET-18-OMe.
KeywordsCell Cycle Distribution Ether Lipid Kill Cancer Cell Propidium Iodide Fluorescence Cancer Chemotherapeutic Agent
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