Immunobiochemical and Molecular Biological Studies on Arachidonate 12-Lipoxygenase of Porcine Leukocytes
Arachidonate 12-lipoxygenase is a dioxygenase which incorporates one molecule of oxygen into arachidonic acid regiospecifically and stereospecifically, and produces 12S-hydroperoxy-5,8,10,14-eicosatetraenoic acid (Figure 1). Since the enzyme was found in platelets as the first mammalian lipoxygenase (1,2), the 12-lipoxygenase has been found in a variety of tissues of a number of animal species (3,4). In addition to 12-lipoxygenase, several more lipoxygenases have been found in mammalian tissues. It is well known that the leukotriene synthesis is initiated by 5-lipoxygenase and the biosynthesis of prostaglandin and thromboxane by cyclooxygenase. These compounds have specific biological activities which regulate the functions of various animal tissues, and their biological functions have been generalized for a variety of animal species and tissues. However, no bioactive compound with such a general function has so far been found in the 12-lipoxygenase pathway of arachidonate metabolism (5).
KeywordsArachidonic Acid Broad Substrate Specificity Immunoaffinity Chromatography Eicosatetraenoic Acid Specific Biological Activity
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