Partitioning of Gramicidin A’ Between Coexisting Phases within Phospholipid Bilayers
We investigated the partitioning behavior of gramicidin A′ in various binary phospholipid mixtures in which gel and liquid crystalline phase coexistence had been induced. The quenching of the gramicidin A′ tryptophanyl fluorescence by a spin-labeled phosphatidylcholine was used to determine the equilibrium ratio of gramicidin A′ concentration in the liquid crystalline phase to that in the gel phase (i.e. the partition coefficient, KP). Three multilamellar vesicular systems, differing in the order and rigidity of the gel phase, were compared. The phospholipid content of the gel phase was rich in dipalmitoylphosphatidylcholine or distearoylphosphatidylcholine, or was entirely Ca(dioleoylphosphatidylserine)2. In all cases the gel phase was depleted of gramicidin A′, with KP = 10 ± 2, 25 ± 5, and ≥30, respectively. These data suggest that the more ordered the gel phase, the less it can accommodate a transmembrane polypeptide.
KeywordsElectron Paramagnetic Resonance Mole Fraction Coat Protein Fluorescence Quenching Liquid Crystalline Phase
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