Abstract
It has been known for about 30 years that the inactivation of biologically active macromolecules irradiated in vitro with X or gamma rays exhibit the dose-response phenomenon illustrated in Fig. 1. As shown in the figure, in pure buffer (control) the inactivation proceeds more or less at the same rate in the absence or in the presence of oxygen, as is very frequently observed with biological macromolecules such as DNA and enzymes (1). When a thiol is added to the solution, a marked oxygen effect becomes apparent.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
M. Quintiliani, The oxygen effect in radiation inactivation of DNA and enzymes. Int. J. Radiat. Biol. 50:573–594 (1986).
P. Howard-Flanders, Effect of oxygen on the radio-sensitivity of bacteriophage in the presence of sulphydryl compounds. Nature 186: 485–487 (1960).
M. Tamba, M. Quintaliani, and G. Simone, Interactions of thiyl free radicals with oxygen: a pulse radiolysis study. Int. J. Radiat. Biol. 50:595–600 (1986).
M. Tamba, G. Simone, and M. Quintaliani, Radiation induced reactions of glutathione with oxygen and their possible role in biological systems. In: Anticarcinogenesis and Radiation Protection, 1987 (P.A. Cerutti, 0.F. Nygaard and M.G. Simic, Eds.), pp. 25–32. Plenum, New York.
G.W. Schwert and Y. Takenaka, A spectrophotometric determination of trypsin and chymotrypsin. Biochem. Biophys. Acta 16:570–575 (1985).
L.G. Augenstine, The effects of ionizing radiation on enzymes. Adv. Enzymol. 24:359–413 (1962).
M. Tamba, G. Simone, and M. Quintaliani (manuscript in preparation).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media New York
About this chapter
Cite this chapter
Simone, G., Tamba, M., Quintiliani, M. (1991). Oxygen and Radical Scavengers in Radiation-Inactivation of Trypsin in Vitro . In: Nygaard, O.F., Upton, A.C. (eds) Anticarcinogenesis and Radiation Protection 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3850-9_35
Download citation
DOI: https://doi.org/10.1007/978-1-4615-3850-9_35
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6718-5
Online ISBN: 978-1-4615-3850-9
eBook Packages: Springer Book Archive