Formation of 8-Hydroxydeoxyguanosine in DNA and Carcinogenesis

  • Hiroshi Kasai
  • Myung-Hee Chung
  • Haruo Kiyosawa
  • Eiko Ohtsuka
  • Susumu Nishimura


Oxygen radicals are produced by ionizing radiation and many other environmental carcinogens. They are also produced endogenously in cells by oxygen metabolism. It seems important therefore to study DNA damage by oxygen radicals and its relation to carcinogenesis.


Cigarette Smoke Condensate Potassium Bromate Produce Oxygen Radical Human Peripheral Blood Cell Thymine Glycol 
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  1. 1.
    H. Kasai, H. Hayami, Z. Yamaizumi, H. Saito, and S. Nishimura, Detection and identification of mutagens and carcinogens as their adducts with guanosine derivatives. Nucleic Acids Res. 12:2127–2136 (1984).PubMedCrossRefGoogle Scholar
  2. 2.
    H. Kasai and S. Nishimura, Hydroxylation of deoxyguanosine at the C-8 position by ascorbic acid and other reducing agents. Nucleic Acids Res. 12:2137–2145 (1984).PubMedCrossRefGoogle Scholar
  3. 3.
    H. Kasai, H. Tanooka, and S. Nishimura, Formation of 8-hydroxyguanine residues in DNA by X-irradiation. Gann 75:1037–1039 (1984).PubMedGoogle Scholar
  4. 4.
    H. Kasai and S. Nishimura, Hydroxylation of deoxyguanosine at the C-8 position by polyphenols and aminophenols in the presence of hydrogen peroxide and ferric ion. Gann 75:565–566 (1984).PubMedGoogle Scholar
  5. 5.
    H. Kasai and S. Nishimura, DNA damage induced by asbestos in the presence of hydrogen peroxide. Gann 75:841–844 (1984).PubMedGoogle Scholar
  6. 6.
    H. Kasai and S. Nishimura, Formation of 8-hydroxydeoxyguanosine in DNA by auto-oxidized unsaturated fatty acid. In: Medical, Biochemical and Chemical Aspects of Free Radicals 1988 (O. Hayaishi, E. Niki, M. Rondo, and T. Yoshikawa, Eds.), pp. 1021–1023 Elsevier, Amsterdam.Google Scholar
  7. 7.
    Y. Kuchino, F. Mori, H. Kasai, H. Inoue, S. Iwai, K. Miura, E. Ohtsuka, and S. Nishimura, Misreading of DNA templates containing 8-hydroxydeoxyguanosine at the modified base and at adjacent residues. Nature 327:77–79 (1987).PubMedCrossRefGoogle Scholar
  8. 8.
    H. Kasai, P.F. Crain, Y. Kuchino, S. Nishimura, A. Ootsuyama, and H. Tanooka, Formation of 8-hydroxydeoxyguanosine moiety in cellular DNAby agents producing oxygen radicals and evidence for its repair. Carcinogenesis 7:1849–1851 (1986).PubMedCrossRefGoogle Scholar
  9. 9.
    R.A. Floyd, J.J. Watson, P.K. Wong, D.H. Altimiller, and R.C. Rickard, Hydroxyl free radical adduct of deoxyguanosine: Sensitive detection and methods of formation. Free Radical Res. Commun. 1:163–172 (1986).CrossRefGoogle Scholar
  10. 10.
    H. Kasai, S. Nishimura, Y. Kurokawa, and Y. Hayashi, Oral administration of the renal carcinogen, potassium bromate, specifically produces 8-hydoxydeoxyguanosine in rat target organ DNA. Carcinogenesis 8:1959–1961 (1987).PubMedCrossRefGoogle Scholar
  11. 11.
    H. Kasai, Y. Okada, S. Nishimura, M.S. Rao, and J.K. Reddy, Formation of 8-hydroxydeoxyguanosine in liver DNA of rats following long-term exposure to a peroxisome proliferator. Cancer Res. 49:2603–2605 (1989).PubMedGoogle Scholar
  12. 12.
    H. Kiyosawa, M. Suko, H. Okudaira, K. Murata, T. Miyamoto, M.-H. Chung, H. Kasai, and S. Nishimura, Cigarette smoking induces formation of 8hydroxydoexyguanosine, one of the oxidative DNA damages in human peripheral leucocyte. Free Radical Res. Commun. (in press).Google Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Hiroshi Kasai
    • 1
  • Myung-Hee Chung
    • 1
  • Haruo Kiyosawa
    • 1
  • Eiko Ohtsuka
    • 2
  • Susumu Nishimura
    • 1
  1. 1.Biology DivisionNational Cancer Center Research InstituteTokyo 104Japan
  2. 2.Faculty of Pharmaceutical SciencesHokkaido UniversitySapporo 060Japan

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