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Expression and Regional Induction of c-fos Gene in the Central Nervous System After Brain Injury

  • Sabera Ruzdijic
  • Selma Kanazir
  • Mirjana Stojiljkovic
  • Ljubisav Rakic

Abstract

The proto-oncogene c-fos encodes a nuclear phosphoprotein which is thought to act as transcriptional regulatory protein for a specific set of genes (1-4). The c-fos is able to form a complex with other proteins, one of which was recently identified as the c-jun protein. This complex (c-fos and c-jun heterodimer) has DNA binding properties, and its DNA target sequence was recently shown to be the activator protein-1 (AP-1) binding site (5,6). The strong induction of c-fos expression after growth factor stimulation suggests that the c-fos protein may be involved in the regulation of specific sets of genes controlling growth and differentiation (7,8). Moreover, the presence and induction of the c-fos gene in the adult nervous system suggest that its protein product may play a role in neuronal plasticity. In the brain, c-fos protein is present in basal amounts and is elevated by many sorts of stimuli. For example, it is rapidly and transiently induced in hippocampus and other regions by seizure activity (9-11). A specific pattern of c-fos immunoreactivity was observed after cortical stimulation in adult brain (12) and after sensory stimulation in spinal cord (13). However, there is also some evidence that c-fos induction could be a consequence of stress or physical damage to the brain (14).

Keywords

Transcriptional Regulatory Protein Cortical Injury Nucleus Caudatus Left Nucleus Growth Factor Induce Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    B.R. Franza Jr., F.J. Rauscher III, S.F. Josephs and T. Curran. The Fos-complex and Fos-related antigens recognize sequence elements that contain AP-1 site. Science 239, 1150–1153 (1988).PubMedCrossRefGoogle Scholar
  2. 2.
    F.J. Rauscher III, L.C. Sambucetti, T. Curran, R.J. Distel and B.M. Spiegelman. Common DNA binding site for Fos protein complexes and transcription factor AP-1. Cell 52, 471–480 (1988).PubMedCrossRefGoogle Scholar
  3. 3.
    J.D. Milbrandt. A nerve growth factor induced gene encodes a possible transcriptional regulatory factor. Science 238, 797–799 (1987).PubMedCrossRefGoogle Scholar
  4. 4.
    K. Ryder, L.F. Lau and D. Nathans. A gene activated by growth factors is related to the oncogene v-jun. Proc. Natl. Acad. Sci. USA 85, 1487–1491 (1988).CrossRefGoogle Scholar
  5. 5.
    T. Curran and B.R. Branza Jr. Fos and Jun: The AP-1 connection. Cell 55, 395–397 (1988).PubMedCrossRefGoogle Scholar
  6. 6.
    P. Vogt and R. Tijan. Jun: A transcriptional regulator turned oncogenic. Oncogene 3, 3–7 (1988).Google Scholar
  7. 7.
    R. Muller, R. Bravo, J. Burckardt and T. Curran. Induction of c-fos gene and protein by growth factors precedes activation of c-myc. Nature 312, 710–720 (1984).Google Scholar
  8. 8.
    M.E. Greenberg and E.B. Ziff. Stimulation of 3T3 cells induces transcription of the c-fox proto-oncogene. Nature 311, 433–438 (1989).CrossRefGoogle Scholar
  9. 9.
    J.I. Morgan, D.R. Cohen, J.L. Hempstead and T. Curran. Mapping patterns of c-fos expression in the central nervous system after seizure. Science 237, 192–197 (1987).PubMedCrossRefGoogle Scholar
  10. 10.
    M. Dragunow and H.A. Robertson. Kindling stimulation induced c-fos protein(s) in granule cells of the rate dentate gyrus. Nature 329, 441–442 (1987).PubMedCrossRefGoogle Scholar
  11. 11.
    M. Dragunow and H.A. Robertson. Generalized seizures induce c-fos protein(s) in mammalian neurons. Neurosci. Lett. 82, 157–161 (1987).Google Scholar
  12. 12.
    S.M. Sagar, F.R. Sharp and T. Curran. Expression of c-fos protein in brain: metabolic mapping at the cellular level. Science 240, 1328–1331 (1988).PubMedCrossRefGoogle Scholar
  13. 13.
    S.P. Hunt, A. Pini and G. Evan. Induction of c-fos protein in spinal cord neurons following sensory stimulation. Nature 328, 631–634 (1987).CrossRefGoogle Scholar
  14. 14.
    R.M. Gubits. C-fos gene expression is induced by heat shock. J. Cell Biol. 103, 166–171 (1986).Google Scholar
  15. 15.
    Lj. Rakic. Experimental models of restoration of functions after brain lesions. In: Recovery of Function in the Nervous System (F. Cohadon and J. Lobo Antunex, Eds.), Fidia Research Series, Vol. 13, pp. 67–82. Liviana Press, Padova, 1988.Google Scholar
  16. 16.
    V. Glisin, R. Crkvenjakov and C. Byrus. Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry 13, 2633–2636 (1974).PubMedCrossRefGoogle Scholar
  17. 17.
    J.D. White and C.M. Gall. Differential regulation of neuropeptides and proto-oncogene mRNA content in the hippocampus following recurrent seizures. Mol. BrainRes. 3, 21–29 (1987).Google Scholar
  18. 18.
    A.A.P. Leao. Spreading depression of activity in the cerebral cortex. J. Neurophysiol. 7, 359–390 (1944).Google Scholar
  19. 19.
    A.J. Hansen. Effect of anoxia on ion distribution in the brain. Physiol. Rev. 65, 101–148 (1985).Google Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Sabera Ruzdijic
    • 1
  • Selma Kanazir
    • 1
  • Mirjana Stojiljkovic
    • 1
  • Ljubisav Rakic
    • 2
  1. 1.Dept. of NeurobiologyInst. for Biological Res.Yugoslavia
  2. 2.Clinical Center of the School of MedicineBelgradeYugoslavia

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