Abstract
Photosynthesis is a complex process, the study of which requires the converging use of many experimental methods (see the Introduction in Chapter 10). As a directly detectable molecular property, fluorescence is largely used in physical chemistry. In photosynthesis, however, fluorescence is mainly (but not only) utilized as an indirect method, based on the early discovery that, in plant chloroplasts and algae, the fluorescence yield of chlorophyll a is mainly controlled by the redox state of the Photosystem-II (PS-Π) reaction center.
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References
Allen, J.F., J. Bennett, K.E. Steinback and C.J. Arntzen (1981) Chloroplast protein phosphorylation couples plastoquinone redox state to distribution of excitation energy between photosystems. Nature 291, 25–29.
Bradbury, Μ and N.R. Baker (1981) Analysis of the slow phases of the in vivo chlorophyll fluorescence induction curve. Changes in the redox state of Photosystem II electron acceptors and fluorescence emission from Photosystem I and II. Biochim. Biophys. Acta 63, 542–551.
Briantais, J. Μ., C. Vernotte, G.H. Krause and E. Weis (1985) Chlorophyll fluorescence of higher plants: chloroplasts and leaves. In Light Emission by Plants and Bacteria (Edited by A.J. Govindjee and D.C. Fork ), Academic Press, NY.
Cadahia, E., J.M. Ducruet and P. Gaillardon (1982) Whole leaf fluorescence as a quantitative probe of detoxification of the herbicide chlortoluron in wheat. Chemosphere 11, 445–450.
Ducruet, J.M., P. Gaillardon and J. Vienot (1984) Use of chlorophyll fluorescence induction kinetics to study translocation and detoxication of DCMU-type herbicides in plant leaves. Z. Naturforsch. 39c, 354– 358.
Ducruet, J.M. and D.R. Ort (1988) Enhanced susceptibility of photosynthesis to high leaf temperature in triazine-resistant Solanum nigrum L. Evidence for Photosystem II D1 protein site of action. Plant Science, 56, 39–48.
Duysens, L.N.M. and H.E. Sweers (1963) Mechanism of two photochemical reactions in algae as studied by means of fluorescence. In Studies on Microalgae and Photosynthetic bacteria, pp. 353–372, University of Tokyo Press, Tokyo.
Etienne, A.L. and J. Lavergne (1972) Action du m-dinitrobenzene sur 1a phase thermique d’induction de fluorescence en photosynthèse. Biochim. Biophys. Acta 283, 268–278.
Genty, Β., J.M. Briantais and N.R. Baker (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim. Biophys. Acta 990, 87–92.
Govindjee, A.J. and D.C. Fork (1986) Light emission by plants and bacteria. Academic Press, NY.
Krause, G.H. and E. Weis (1984) Chlorophyll fluorescence as a tool in plant physiology. Interpretation of fluorescence signals. Photosynth. Res. 5, 139–157.
Lavergne, J. (1974) Fluorescence induction in algae and chloplasts. Photochem. Photobiol. 20, 377–386.
LavorelJ. and A.L. Etienne (1972) in vivo chlorophyll fluorescence. In Primary Processes of Photosynthesis. (Edited by J. Barber), pp. 203–268. Elsevier (North Holland Biomedical Press).
Lavorel, J. and P. Joliot (1972) A connected model of the photosynthetic unit. Biophys. J. 12, 815–831.
Lichtenthaler, H.K. (1988). Applications of Chlorophyll Fluorescence in Photosynthesis Research, Stress Physiology, Hydrobiology and Remote Sensing. Kluwer Academic Publ., Dordrecht.
Mathis, P. (1990) Optical techniques in the study of photosynthesis. In Methods in Plant Biochemistry:, Vol. 4: Lipids, Membranes and Light Receptors. (Edited by Mathis, P ) pp. 231–258, Academic Press.
Melis, A. and L.N.M. Duysens (1978) Biphasic energy conversion kinetics and absorbance difference spectra of Photosystem II of chloroplasts. Evidence for two different Photosystem II reaction centers. Photochem. Photobiol. 29, 373–382.
Melis, A. and P.H. Homann (1976) Heterogeneity of the photochemical centers in system II of chloroplasts. Photochem. Photobiol. 23, 343–350.
Mohanty, N., I. Vass and S. Demeter (1989) Copper toxicity affects Photosystem II electron transport at the secondary quinone acceptor QB. Plant Physiol. 90, 175–179.
Neubauer, C. and U. Schreiber (1987) The polyphasic rise of chlorophyll fluorescence upon onset of strong illumination: I. Saturation characteristics and partial control by the Photosystem II acceptor side. Z. Naturforsch. 42c, 1246–1254.
Quick, W.P. and P. Horton (1984) Studies on the induction of chlorophyll fluorescence quenching by redox state and transthylakoid pH gradient. Proc. Roy. Soc. (Lond.) Β 220, 371–382.
Renganathan, M. and S. Bose (1990) Inhibition of Photosystem II activity by Cu++ ion. Choice of buffer and reagent is critical. Photosynth. Res. 23, 95–99.
Schmidt, W., U. Schreiber and W. Urbach (1988) SO2 injury in intact leaves, as detected by chlorophyll fluorescence. Z. Naturforsch. 43c, 269–274.
Schreiber, U. (1986) Detection of rapid induction kinetics with a new type of high-frequency modulated chlorophyll fluorometer. Photosynth. Res. 9, 261–272.
Schreiber, U. and C. Neubauer (1987) The polyphasic rise of chlorophyll fluorescence upon onset of strong continuous illumination: Π. Partial control by the Photosystem II donor side and possible ways of interpretation. Z. Naturforsch. 42c, 1255–1264.
Schreiber, U., U. Schliwa and W. Bilger (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth. Res. 10, 51–62.
Somersalo, S. and G.H. Krause (1990) Reversible photoinhibition of unhardened and cold-acclimated spinach leaves at chilling temperatures. Planta 180, 181–187.
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Ducruet, JM., Mathis, P. (1991). Chlorophyll Fluorescence Transients in Chloroplasts and Leaves. In: Valenzeno, D.P., Pottier, R.H., Mathis, P., Douglas, R.H. (eds) Photobiological Techniques. NATO ASI Series, vol 216. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3840-0_11
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DOI: https://doi.org/10.1007/978-1-4615-3840-0_11
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