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Mechanisms of Environmental Mutagenesis-Carcinogenesis pp 117–128Cite as

The Adaptive Response to Alkylation Damage in Escherichia Coli

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Abstract

E. coli has both non-inducible and inducible DNA repair activities which remove deleterious lesions from methylated DNA. The non-inducible Tag and Ogt proteins repair toxic 3-methyladenine and mutagenic 06-methylguanine DNA lesions, repsectively. Induction of the adaptive response increases expression of four genes, ada, alkA, alkB, and aidB. The Ada and AlkA proteins are well characterised but the functions of the AlkB and AidB proteins are unknown. The AlkA protein in a DNA glycosylase which excises 3-methyladenine and several other minor lesions from DNA. The Ada protein is a DNA-methyltransfrerase which transfers the methyl groups from 06-methylguanine and methylphosphotriesters on to two of its own cysteine residues. The Ada protein self-methylated by repair of a methylphosphotriester is a strong transcriptional activator of the inducible genes of the adaptive response. The active sites of the multifunctional Ada protein have been identified and studies of the regulatory domains initiated.

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Authors and Affiliations

  1. Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Potters Bar, Herts, EN6 3LD, UK

    Barbara Sedgwick

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  1. Barbara Sedgwick
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  1. Institute of Biology, National Research Center “Democritus”, 153 10, Athens, Greece

    Andreas Kappas

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© 1990 Springer Science+Business Media New York

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Sedgwick, B. (1990). The Adaptive Response to Alkylation Damage in Escherichia Coli . In: Kappas, A. (eds) Mechanisms of Environmental Mutagenesis-Carcinogenesis. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3808-0_9

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  • DOI: https://doi.org/10.1007/978-1-4615-3808-0_9

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-6698-0

  • Online ISBN: 978-1-4615-3808-0

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