Current Techniques for Human Population Monitoring for Genetic Effects

  • A. T. Natarajan
  • A. D. Tates

Abstract

Exposure of human populations to genotoxic agents can lead to genetic effects which can be monitored in somatic cells. Lymphocytes and erythrocytes from human peripheral blood are the target cells which can be easily obtained and studied. The consequences of exposure of these cells during circulation as well as effects to their stem cells in the bone marrow can be estimated by either taking blood samples shortly or longer times after the event. The end points that are currently studied are (1) chromosomal aberrations and micronuclei in lymphocytes, (2) Sister chromatid exchanges in lymphocytes (3) aneuploidy in lymphocytes, (4) HPRT-mutations in lymphocytes, (5) glycophorin mutations in erythrocytes and (6) haemoglobin mutations in erythrocytes. The current techniques to estimate these end points will be described briefly and the relative merits of these techniques will be discussed.

Keywords

Codon Tate Sorting Purine Bleomycin 

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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • A. T. Natarajan
    • 1
  • A. D. Tates
    • 1
  1. 1.Dept. Radiation Genetics and Chemical Mutagenesis, Sylvius Laboratory, Medical FacultyState University of LeidenLeidenThe Netherlands

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