An Independent Haemostatic Mechanism: Shear Induced Platelet Aggregation

  • J. R. O’Brien
  • G. P. Salmon
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 281)

Abstract

We have published (1) evidence indicating that high shearing forces alone applied to platelets expose and activate a unique domain on glycoprotein IIb/IIIa (GPIIb/IIIa) at the platelet surface. In the presence of von Willebrand’s factor (vWf) and divalent cations the platelets will aggregate. This paper reviews the extensive literature on high shear effects. It describes a device in which high shear produced by forcing heparinised whole blood through a complex filter normally results in platelet activation; the platelets aggregate and then block the filter. This system is inhibited by antibodies to GPIIb/IIIa and to vWf: fibrinogen is apparently not involved. The same antibodies to GPIIb/IIIa and vWf prevent high shear induced thrombosis occurring in vivo in animal models. The filter blockage is not influenced by aspirin, heparin and ticlopidine and so involves a different mechanism from the aspirin sensitive mechanisms involved in clinical thrombosis prevention in vivo in man. While there are a number of unexplained phenomena in this global test nevertheless this filter model is a simple way of studying a recently recognised pathway which is almost certainly involved in thrombogenesis in man.

Keywords

Catheter Anisotropy Europe EDTA Aspirin 

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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • J. R. O’Brien
    • 1
  • G. P. Salmon
    • 1
  1. 1.Central LaboratorySt. Mary ’s HospitalPortsmouth, HantsUK

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