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A New Trisaccharide Sugar Chain Linked to a Serine Residue in the First EGF-Like Domain of Clotting Factors VII and IX and Protein Z

  • Sadaaki Iwanaga
  • Hitoshi Nishimura
  • Shun-ichiro Kawabata
  • Walter Kisiel
  • Sumihiro Hase
  • Tokuji Ikenaka
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 281)

Abstract

Recently, we determined the complete amino acid sequence of bovine factor VII (Takeya, H. et al. (1988) J. Biol. Chem. 263, 14868–14877). In the course of the studies, we found an unknown serine derivative at position 52 in the first epidermal growth factor-like domain of factor VII. A pentapeptide isolated from the S-aminoethylated factor VII contained Ser-52, which could not be identified with a gas-phase sequencer. The same results were also obtained for a pentapeptide containing Ser-53 of factor IX and protein Z. Component sugar analysis revealed that the peptide contained 1 mol of glucose and 2 mol of xylose. This sugar component was also confirmed by high-resolution fast atom bombardment mass spectrometric analysis of the pentapeptide. The trisaccharide was released from the peptides by means of ß-elimination reaction and its reducing end was identified as pyridylamino-glucose. These results indicate the existence of a (Xy12)Glc-Ser structure in factors VII, IX and protein Z. Similar results were obtained for human factors VII, IX and protein Z. This is the first report of a (Xy12)-Glc-Ser structure in glycoproteins to our knowledge. The presence of the unique trisaccharide structure in factors VII, IX and protein Z leads us to anticipate its biological role in the tissue factor pathway.

Keywords

Sugar Chain Bovine Protein Mouse Epidermal Growth Factor Trisaccharide Chain Amino Amino 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1990

Authors and Affiliations

  • Sadaaki Iwanaga
    • 1
  • Hitoshi Nishimura
    • 1
  • Shun-ichiro Kawabata
    • 1
  • Walter Kisiel
    • 2
  • Sumihiro Hase
    • 3
  • Tokuji Ikenaka
    • 3
  1. 1.Department of BiologyFaculty of Science Kyushu University 33FukuokaJapan
  2. 2.Blood System Foundation Laboratory Department of PathologyUniversity of New Mexico School of MedicineAlbuquerqueUSA
  3. 3.Department of ChemistryOsaka University College of ScienceOsakaJapan

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