High-Level Expression of Foreign Genes in Mammalian Cells

  • Susan E. Kane
Part of the Genetic Engineering book series (GEPM, volume 13)

Abstract

Gene expression systems which function in eukaryotic cells are finding an increasing number of applications in modern-day somatic cell genetics and cell biology. For example, as new genes are cloned from a variety of species and cell types, their corresponding gene products can be studied in defined cell lines. For biochemical analysis, it is often necessary to overproduce and purify large amounts of a protein from cells which support appropriate post-translational modification, such as glycosylation or phosphorylation. In addition, specialized cell lines are now being used for production of custom-made proteins, as in the case of myelomas and genetically engineered immunoglobulins (1). Structure-function studies ultimately rely on the analysis of mutated forms of a protein in their appropriate cellular environment.

Keywords

Adenosine Methotrexate Folate Glucocorticoid Phenylalanine 

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Susan E. Kane
    • 1
  1. 1.City of Hope National Medical CenterDuarteUSA

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