Model System Studies on Photosensitization in Light Scattering Media
Biological photosensitization is being investigated in tissue models consisting of a sensitizing dye, a biological target, and light scattering particles. Results are reported for a dihematoporphyrin ether-subtilisin Carlsberg-polystyrene microsphere system in which the rate of photodynamic enzyme inactivation was measured at different wavelengths and scatterer concentrations. The optical constants were calculated with the one-dimensional diffusion approximation, from flux profiles measured with a fiber optic probe. Preliminary results arereported on lipid peroxidation in a dihematoporphyrin ether-liposome-polystyrene microsphere system. A two-channel integrating sphere spectrophotometer with on-line computer data acquisition was used to determine the optical constants of several model systems.
KeywordsEthyl Mercury Attenuation Bromide Polystyrene
Unable to display preview. Download preview PDF.
- Blan, Q. A. and Grossweiner, L. I., 1987, Photocnem. Photobiol., 45, 77.Google Scholar
- Bohren, C. F. and Huffman, D. R., 1983, Absorption and Scattering of Light by Small Particles, (New York: John Wiley & Sons).Google Scholar
- Chandrasekhar, S., 1950, Radiative Transfer (Oxford: Oxford University Press).Google Scholar
- Ishimaru, A., 1978 Wave Propagation and Scattering in Random Media, Vol.1 (New York: Academic Press).Google Scholar
- Jacques, S. L., Alter, C. A., and Prahl, S. A., 1987, Lasers Life. Scis., 1, 309.Google Scholar
- Kerker, M., 1969, The Scattering of Light, (New York: Academic Press).Google Scholar
- Schifano, M. J. and Grossweiner, L. I., 1988, Photochem. Photobiol., in press.Google Scholar
- Star, W. M., Marihnissen, J. P. A., and van Gemert, J. P. A., 1987, J. Photochem. Photobiol., 1B, 149.Google Scholar