Model System Studies on Photosensitization in Light Scattering Media
Biological photosensitization is being investigated in tissue models consisting of a sensitizing dye, a biological target, and light scattering particles. Results are reported for a dihematoporphyrin ether-subtilisin Carlsberg-polystyrene microsphere system in which the rate of photodynamic enzyme inactivation was measured at different wavelengths and scatterer concentrations. The optical constants were calculated with the one-dimensional diffusion approximation, from flux profiles measured with a fiber optic probe. Preliminary results arereported on lipid peroxidation in a dihematoporphyrin ether-liposome-polystyrene microsphere system. A two-channel integrating sphere spectrophotometer with on-line computer data acquisition was used to determine the optical constants of several model systems.
KeywordsMethylene Blue Optical Constant Tissue Model Polystyrene Microsphere Incident Irradiance
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