Abstract
A vector, termed pZipGptNeo, has been constructed by the introduction of a DNA fragment containing the E. coli gpt gene into a retroviral shuttle vector. The pZipGptNeo vector was then introduced into mouse L cells to construct the A912 cell line. This cell line stably expresses the gpt gene and contains a single copy of the vector integrated into its chromosomal DNA. Studies utilizing the A912 cell line to determine the specificity of spontaneous and 5-bromodeoxyuridine-induced mutations will be summarized. The role of the surrounding DNA sequence in the production of these mutations will be discussed. The construction of a new retroviral shuttle vector and its introduction into the CHO-Kl cell line will be described. Preliminary experiments suggest that spontaneous gpt gene mutations arising in CHO cells are similar to those seen in mouse L cells. A small region of the gene which was shown to be a hotspot for spontaneous mutation in the mouse cells is apparently also a hotspot in Chinese hamster cells.
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© 1991 Springer Science+Business Media New York
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Ashman, C.R. (1991). Mutational Hotspots in Mammalian Cells. In: Riklis, E. (eds) Photobiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3732-8_24
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DOI: https://doi.org/10.1007/978-1-4615-3732-8_24
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