Abstract
Although targeted UV-radiation mutagenesis appears to require both the recA and umuC genes in Escherichia coli, examples of recA-dependent but umuC-independent mutagenesis exist, e.g., gamma-radiation mutagenesis (Mutat. Res. 128, 1, 1984) and streptozotocin mutagenesis (Mutat. Res. 166, 229, 1986). Most of the information on umuC-independent mutagenesis comes from studies on ionizing radiation mutagenesis. These results will be reviewed here. Analyses of the various suppressor and back mutations that result in argE3 and hisG4 ochre reversion and an analysis of trpE9777(+1 frameshift) reversion were performed on umuC and wild-type cells gamma irradiated in the presence and absence of oxygen. In wild-type cells, the presence of oxygen enhances gamma-radiation mutagenesis. Although the umuC strain showed the gamma-radiation induction of base substitution and frameshifts when irradiated in the absence of oxygen, the umuC mutation blocked all oxygen-dependent base-substitution mutagenesis, but not all oxygen-dependent frameshift mutagenesis. For anoxically-irradiated cells, the yields of GC→AT and AT→GC transitions were largely umuC independent, while the yields of (AT or GC)→TA transversions were heavily umuC dependent. Therefore, the data for anoxically-irradiated cells support the hypothesis that gamma irradiation produces two kinds of DNA lesions that require recA-dependent misrepair to induce mutations. For base-substitution mutagenesis, one kind of lesion requires the umuC gene and produces transversion mutations, while a second kind of lesion produces transition mutations and does not require the umuC gene. For cells irradiated in the presence of oxygen, there seems to be an additional kind of lesion whose mutagenic potential for base substitutions (but not frameshifts) is completely dependent on the umuC gene.
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References
Ayaki, H., Yamamoto, O., and Sawada, S., 1987, Role of the main cytosine radiolytic product in ionizing radiation-induced mutagenesis. Journal of Radiation Research, 28, 254–261.
Breimer, L.H., and Lindahl, T., 1985, Enzymatic excision of DNA bases damaged by exposure to ionizing radiation or oxidizing agents. Mutation Research, 150, 85–89.
Burns, P.A., Gordon, A.J.E., and Glickman, B.W., 1987, Influence of neighbouring base sequence on N-methyl-N’-nitro-N-nitrosoguanidine mutagenesis in the lacI gene of Escherichia coli. Journal of Molecular Biology, 194, 385–390.
Elledge, S.J., and Walker, G.C., 1983, Proteins required for ultraviolet light and chemical mutagenesis. Identification of the products of the umuC locus of Escherichia coli. Journal of Molecular Biology, 164, 175–192.
Fram, R.J., Sullivan, J., and Marinus, M.G., 1986, Mutagenesis and repair of DNA damage caused by nitrogen mustard, N,N’-bis(2-chloroethyl)-N-nitrosourea (BCNU), streptozotocin, and mitomycin C inE. coli. Mutation Research166, 229–242.
Ishii, Y., and Kondo, S., 1975, Comparative analysis of deletion and base-change mutabilities of Escherichia coli B strains differing in DNA repair capacity (wild-type,uvrA, polA, recA)by various mutagens. Mutation Research, 27, 27–44.
Kato, T., and Nakano, E., 1981, Effects of the umuC36 mutation on ultraviolet-radiationinduced base-change and frameshift mutations in Escherichia coli. Mutation Research, 83, 307–319.
Kato, T., and Shinoura, Y., 1977, Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light. Molecular and General Genetics,156, 121–131.
Kato, T., Shinoura, Y., Templin, A., and Clark, A.J., 1980, Analysis of ultraviolet light-induced suppressor mutations in the strain of Escherichia coli K-12 AB1157: An implication for molecular mechanisms of UV mutagenesis. Molecular and General Genetics, 180, 283–291.
Kondo, S., 1968, Mutagenicity versus radiosensitivity in Escherichia coli. Proceedings of the 12th International Congress of Genetics, 2, 126–127.
Kondo, S., Ichikawa, H., Iwo, K., and Kato, T., 1970, Base-change mutagenesis and prophage induction in strains of Escherichia coli with different DNA repair capacities. Genetics, 66, 187–217.
Kunkel, T.A., 1984, Mutational specificity of depurination, Proceedings of the National Academy of Sciences (USA) 81, 1494–1498.
Laspia, M.F., and Wallace, S.S., 1988, Excision repair of thymine glycols, urea residues, and apurinic sites in Escherichia coli. Journal of Bacteriology, 170, 3359–3366.
Miller, J.H., 1983, Mutational specificity in bacteria. Annual Review of Genetics, 17, 215–238.
Rabkin, S.D., Moore, P.D., and Strauss, B.S., 1983 In vitro bypass of UV-induced lesions by Escherichia coli DNA polymerase I: Specificity of nucleotide incorporation. Proceedings of the National Academy of Science (USA), 80, 1541–1545.
Sargentini, N.J., and Smith, K.C., 1984, umuC-Dependent and umuC-independent gamma-and UV-radiation mutagenesis in Escherichia coli. Mutation Research 128, 1–9.
Sargentini, N.J., and Smith, K.C., 1987, Ionizing and ultraviolet radiation-induced reversion of sequenced frameshift mutations in Escherichia coli: a new role for umuDCsuggested by delayed photoreactivation. Mutation Research, 179, 55–63.
Sargentini, N.J., and Smith, K.C., 1989, Mutational spectrum analysis of umuC-independent and umuC-dependent gamma-radiation mutagenesis in Escherichia coli. Mutation Research 211, 193–203.
Schaaper, R.M., Glickman, B.W., and Loeb, L.A., 1982, Mutagenesis resulting from depurination is an SOS process. Mutation Research 106, 1–9.
Schendel, P.F., and Defais, M., 1980, The role of umuC gene product in mutagenesis by simple alkylating agents. Molecular and General Genetics, 177, 661–665.
Shinagawa, H., Kato, T., Ise, T., Makino, K., and Nakata, A., 1983, Cloning and characterization of the umu operon responsible for inducible mutagenesis inEscherichia coli. Gene,23, 167–174.
Shinoura, Y., Ise, T., Kato, T., and Glickman, B.W., 1983, umuC-mediated misrepair mutagenesis in Escherichia coli: extent and specificity of SOS mutagenesis. Mutation Research, 111, 51–59.
Shirname-More, L., Rossman, T.G., Troll, W., Teebor, G.W., and Frenkel, K., 1987, Genetic effects of 5-hydroxymethyl-2’-deoxyuridine, a product of ionizing radiation. Mutation Research 178, 177–186.
Steinborn, G., 1978, uvm Mutants of Escherichia coli K12 deficient in UV mutagenesis. I. Isolation of uvm mutants and their phenotypical characterization in DNA repair and mutagenesis. Molecular and General Genetics,165, 87–93.
Teoule, R., 1987, Radiation-induced DNA damage and its repair. International Journal of Radiation Biology 51, 573–589.
Ullrich, M., and Hagen, U., 1971, Base liberation and concomitant reactions in irradiated DNA solutions. International Journal of Radiaton Biology, 19, 507–517.
Walker, G.C., 1977, Plasmid (pKM101)-mediated enhancement of repair and mutagenesis: dependence on chromosomal genes in Escherichia coli K-12. Molecular and General Genetics, 152, 93–103.
Walker, G.C., 1984, Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli. Microbiological Reviews, 48, 60–93.
Walker, G.C., and Dobson, P.P., 1979, Mutagenesis and repair deficiences of Escherichia coli umuC mutants are suppressed by the plasmid pKM101. Molecular and General Genetics, 172, 17–24.
Witkin, E.M., 1976, Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriological Reviews, 40, 869–907.
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© 1991 Springer Science+Business Media New York
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Smith, K.C., Sargentini, N.J. (1991). umuC-Independent, recA-Dependent Mutagenesis. In: Riklis, E. (eds) Photobiology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-3732-8_21
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DOI: https://doi.org/10.1007/978-1-4615-3732-8_21
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