An Archaebacterial Antigen Used to Study Immunological Humoral Response to C-Myc Oncogene Product
We have previously shown that it is possible to detect by Western blot analysis antibodies against the hyman c-niyc oncogene product in the serum of some cancer patients using as antigen an 84 kD protein obtained from Halohacterium halobium . To study whether the presence of circulating anti-c-mvc antibodies might be more strongly associated with a specific type of cancer, sera of 28 colorectal cancer patients at the beginning of their treatment at the “Institut Curie” were tested. We observed a significantly higher level of positivity (18 out of 28) than in other cancer patient sera.
This conclusion obtained with an archaebacterial antigen was further consolidated by experiments in which we used the purified c-myc protein produced in E. coli by genetic engineering.
KeywordsColorectal Cancer Patient Positive Seron Cellular Oncogene Halobacterium Halobium Cancer Patient Seron
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