An Archaebacterial Antigen Used to Study Immunological Humoral Response to C-Myc Oncogene Product

  • Kamel Ben-Mahrez
  • Irène Sorokine
  • Dominique Thierry
  • Toshiyuki Kawasumi
  • Shunsuke Ishii
  • Remy Salmon
  • Masamichi Kohiyama
Part of the NATO ASI Series book series (NSSA, volume 201)


We have previously shown that it is possible to detect by Western blot analysis antibodies against the hyman c-niyc oncogene product in the serum of some cancer patients using as antigen an 84 kD protein obtained from Halohacterium halobium [1]. To study whether the presence of circulating anti-c-mvc antibodies might be more strongly associated with a specific type of cancer, sera of 28 colorectal cancer patients at the beginning of their treatment at the “Institut Curie” were tested. We observed a significantly higher level of positivity (18 out of 28) than in other cancer patient sera.

This conclusion obtained with an archaebacterial antigen was further consolidated by experiments in which we used the purified c-myc protein produced in E. coli by genetic engineering.


Colorectal Cancer Patient Positive Seron Cellular Oncogene Halobacterium Halobium Cancer Patient Seron 
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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Kamel Ben-Mahrez
    • 1
  • Irène Sorokine
    • 1
  • Dominique Thierry
    • 2
  • Toshiyuki Kawasumi
    • 1
  • Shunsuke Ishii
    • 3
  • Remy Salmon
    • 4
  • Masamichi Kohiyama
    • 1
  1. 1.Institut Jacques MonodUniversité Paris VIIParis Cedex 05France
  2. 2.CEA, IPSN, DPS, SHRCedexFrance
  3. 3.Riken Tsukuba Life ScienceTsukuba-shi, Ibaraki 305Japan
  4. 4.Institut CurieParis Cedex 05France

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