Advertisement

An Archaebacterial Antigen Used to Study Immunological Humoral Response to C-Myc Oncogene Product

  • Kamel Ben-Mahrez
  • Irène Sorokine
  • Dominique Thierry
  • Toshiyuki Kawasumi
  • Shunsuke Ishii
  • Remy Salmon
  • Masamichi Kohiyama
Part of the NATO ASI Series book series (NSSA, volume 201)

Abstract

We have previously shown that it is possible to detect by Western blot analysis antibodies against the hyman c-niyc oncogene product in the serum of some cancer patients using as antigen an 84 kD protein obtained from Halohacterium halobium [1]. To study whether the presence of circulating anti-c-mvc antibodies might be more strongly associated with a specific type of cancer, sera of 28 colorectal cancer patients at the beginning of their treatment at the “Institut Curie” were tested. We observed a significantly higher level of positivity (18 out of 28) than in other cancer patient sera.

This conclusion obtained with an archaebacterial antigen was further consolidated by experiments in which we used the purified c-myc protein produced in E. coli by genetic engineering.

Keywords

Colorectal Cancer Patient Positive Seron Cellular Oncogene Halobacterium Halobium Cancer Patient Seron 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. [1]
    K Ben-Mahrez, D. Thierry, I. Sorokine, A. Danna-Muller and M. Kohiyama, Detection of circulating antibodies against c-mycprotein in cancer patient sera, Br. J. Cancer57: 529 (1988)PubMedCrossRefGoogle Scholar
  2. [2]
    S. N. Sehgal and N. E. Gibbons, Effect of some metal ions on the growth of Halobacterium cutirubrum, Can. J. Microbiol.6: 165 (1960)PubMedCrossRefGoogle Scholar
  3. [3]
    F. W. Studier and B. A. Moffatt, Use of bacteriophase T7 RNA polymerase to direct selective high-level expression of cloned genes, J. Mol. Biol.189: 113 (1986)PubMedCrossRefGoogle Scholar
  4. [4]
    A. L. Schlechter, D. F. Stern, L. Vaidyamathan, S. J. Decker, J. A. Drebin, M. I. Greene and R. A. Weinberg, The neuoncogene: an erb-B-related gene encoding a 185000-Mr tumor antigen, Nature312: 513 (1984)CrossRefGoogle Scholar
  5. [5]
    J. A. Drebin, V. C. Link, R. A. Weinberg and M. I. Greene, Inhibition of tumor growth by a monoclonal antibody reactive with an oncogene-encoded tumor antigen, Proc. Natl. Acad. Sci. USA83: 9129 (1986)PubMedCrossRefGoogle Scholar
  6. [6]
    T. Graf and D. Stehelin, Avian Leukemia viruses. Oncogenes and genome structure, Biochim. Biophys. Acta.651: 245 (1982)PubMedGoogle Scholar
  7. [7]
    K. Bister and H. W. Jansen, Oncogenes in retroviruses and cells: biochemistry and molecular genetics, Adv. Cancer Res.47: 99 (1986)PubMedCrossRefGoogle Scholar
  8. [8]
    G. P. Studzinski, Z. S. Brelvi, S. C. Feldman and R. A. Watt, Participation of c-mycprotein in DNA synthesis of human cells, Science234: 467 (1986)PubMedCrossRefGoogle Scholar
  9. [9]
    K. Kelly, B. H. Cochrane, C. D. Stiles and P. Leder, Cell-specific regulation of the c-mycgene by lymphocyte mitogens and platelet-derived growth factor, Cell35: 603 (1983)PubMedCrossRefGoogle Scholar
  10. [10]
    J. M. Bishop, The molecular genetics of cancer, Science235: 305 (1987)PubMedCrossRefGoogle Scholar
  11. [11]
    H. Land, L. F. Parada and R. A. Weinberg, Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes, Nature304: 596 (1983)PubMedCrossRefGoogle Scholar
  12. [12]
    A. Leder, P. K. Pattengale, A. Kuo, T. A. Stewart and P. Leder, Consequences of widespread deregulation of the c-mycgene in transgenic mice: multiple neoplasms and normal development, Cell45: 485 (1986)PubMedCrossRefGoogle Scholar
  13. [13]
    M. D. Erisman, P. G. Rothberg, R. E. Diehl, C. C. Morce, J. M. Spandorfer and S. M. Astrin, Deregulation of c-mycgene expression in human colon carcinoma is not accompanied by amplification or rearrangement ofthe gene, Mol. Cell. Biol.5: 1969 (1985)PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Kamel Ben-Mahrez
    • 1
  • Irène Sorokine
    • 1
  • Dominique Thierry
    • 2
  • Toshiyuki Kawasumi
    • 1
  • Shunsuke Ishii
    • 3
  • Remy Salmon
    • 4
  • Masamichi Kohiyama
    • 1
  1. 1.Institut Jacques MonodUniversité Paris VIIParis Cedex 05France
  2. 2.CEA, IPSN, DPS, SHRCedexFrance
  3. 3.Riken Tsukuba Life ScienceTsukuba-shi, Ibaraki 305Japan
  4. 4.Institut CurieParis Cedex 05France

Personalised recommendations