Estimation of the Contribution of Archaebacteria and Eubacteria to the Bacterial Biomass and Activity in Hypersaline Ecosystems: Novel Approaches

  • Aharon Oren
Part of the NATO ASI Series book series (NSSA, volume 201)


In order to evaluate the contribution of archaebacteria (the Halobacterium group) and halophilic eubacteria to the bacterial biomass and activity in hypersaline environments I used bile salts, which in very low concentrations cause lysis of halobacteria, while eubacteria and halococci remain intact. The decrease in total bacterial numbers upon bile salt addition is a measure for the community size of bacteria of the Halobacterium group. Bile salts can be used as specific inhibitors for halobacterial activity in measurements of heterotrophic activity of the community. In addition, protein synthesis inhibitors specific for archaebacteria (anisomycin) and eubacteria (chloramphenicol) were used to differentiate between the groups in natural communities.


Bile Salt Bacterial Biomass Hypersaline Environment Heterotrophic Activity Bile Salt Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    F. Rodriguez-Valera, F. Ruiz-Berraquero and A. Ramos-Cormenzana, Characteristics of the heterotrophic bacterial populations in hypersaline environments of different salt concentrations, Microb. Ecol. 7:235 (1981).CrossRefGoogle Scholar
  2. [2]
    F. Rodriguez-Valera, A. Ventosa, G. Juez and J. F. Imhoff, Variation of environmental features and microbial populations with salt concentration in a multi-pond saltern, Microb. Ecol. 11:107 (1985).CrossRefGoogle Scholar
  3. [3]
    B. J. Javor, Planktonic standing crop and nutrients in a saltern ecosystem, Limnol. Oceanogr. 28:153 (1983).CrossRefGoogle Scholar
  4. [4]
    H. P. Dussault, Study of red halophilic bacteria in solar salt and salted fish: I. Effect of Bacto-oxgall, J. Fish. Res. Bd. Canada, 13:183 (1956).CrossRefGoogle Scholar
  5. [5]
    H. P. Dussault, Study of red halophilic bacteria in solar salt and salted fish: II. Bacto-oxgall as a selective agent for differentiation, J. Fish. Res. Bd. Canada, 13:195 (1956).CrossRefGoogle Scholar
  6. [6]
    M. Kamekura, D. Oesterhelt, R. Wallace, P. Anderson and D. J. Kushner, Lysis of halobacteria in Bacto-Peptone by bile acids, Appl. Environ. Microbiol. 54:990 (1988).PubMedGoogle Scholar
  7. [7]
    A. Oren, Estimation of the contribution of halobacteria to the bacterial biomass and activity in solar salterns by the use of bile salts, FEMS Microbiol. Ecol., 73:41 (1990).CrossRefGoogle Scholar
  8. [8]
    A. Oren, The microbial ecology of the Dead Sea, in “Advances in microbial ecology”, Vol. 10, K. C. Marshall, ed., Plenum Publishing Company, New York (1988).Google Scholar
  9. [9]
    T. Pecher and A. Böck, In vivosusceptibility of halophilic and methanogenic organisms to protein synthesis inhibitors, FEMS Microbiol. Lett. 10:295 (1981).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Aharon Oren
    • 1
  1. 1.The Division of Microbial and Molecular Ecology The Institute of Life SciencesThe Hebrew University of JerusalemJerusalemIsrael

Personalised recommendations