Estimation of the Contribution of Archaebacteria and Eubacteria to the Bacterial Biomass and Activity in Hypersaline Ecosystems: Novel Approaches
In order to evaluate the contribution of archaebacteria (the Halobacterium group) and halophilic eubacteria to the bacterial biomass and activity in hypersaline environments I used bile salts, which in very low concentrations cause lysis of halobacteria, while eubacteria and halococci remain intact. The decrease in total bacterial numbers upon bile salt addition is a measure for the community size of bacteria of the Halobacterium group. Bile salts can be used as specific inhibitors for halobacterial activity in measurements of heterotrophic activity of the community. In addition, protein synthesis inhibitors specific for archaebacteria (anisomycin) and eubacteria (chloramphenicol) were used to differentiate between the groups in natural communities.
KeywordsBile Salt Bacterial Biomass Hypersaline Environment Heterotrophic Activity Bile Salt Concentration
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