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Physical Mapping and Gene Transfer Methods for Halobacterium (Haloferax) Volcanii

  • W. Ford Doolittle
  • Robert L. Charlebois
  • Leo C. Schalkwyk
  • Wan. L. Lam
  • R. Keith Conover
  • Dina Tsouluhas
  • Steven W. Cline
  • Jason D. Hofman
  • Annalee Cohen
Part of the NATO ASI Series book series (NSSA, volume 201)

Abstract

Molecular archaebacteriology is now about to enter a manipulative phase. Many laboratories have contributed to the development of methods for proper genetic analysis. Here we describe our efforts with Halobacteriurn volcanii. We have nearly completed a physical map of the genome of this species, have optimized procedures for its transformation by exogenous DNA, and developed shuttle vectors alternately selectable and maintainable in this species and Escherichia coli.

Keywords

Shuttle Vector Anthranilic Acid Gene Transfer Method MluI Site HMGCoA Reductase Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • W. Ford Doolittle
    • 1
  • Robert L. Charlebois
    • 1
  • Leo C. Schalkwyk
    • 1
  • Wan. L. Lam
    • 1
  • R. Keith Conover
    • 1
  • Dina Tsouluhas
    • 1
  • Steven W. Cline
    • 1
  • Jason D. Hofman
    • 1
  • Annalee Cohen
    • 1
  1. 1.Department of BiochemistryDalhousie UniversityHalifaxCanada

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